66 results about "Phytenic acid" patented technology

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and / or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

The invention relates to a chromium-free passivating solution for plated metal and a plating method thereof. The passivating solution comprises 0.001 to 30 g / L of vanadate and 0.01 to 30 g / L of molybdate, and can further comprise an aqueous solution of one or more selected from the group consisting of 1 to 300 g / L of phytic acid, 0.01 to 30 g / L of silane and 1 to 500 g / L of a resin. The passivating solution can be used for plated metal through immersion, spraying, roller coating or brush painting and forms a replacing layer and / or a covering layer on the surface of treated metal, which enables surface anti-corrosion performance of treated metal to be effectively enhanced; in addition, the passivating solution provided in the invention does not contain environment-polluting chromium of any valence state, which is beneficial for environmental protection.

The invention relates to the genetic engineering field, especially to a land bacillus Pedobacter sp. MJ11 with a preservation number of CGMCC No. 2503 and a neutral phytase PHYMJ11, a gene thereof, a recombinant vector containing the same and its application. The invention provides the neutral phytase PHYMJ11 with an amino acid sequence shown as SEQ ID NO. 1 and a gene phyMJ11 for coding said neutral phytase. The neutral phytase obtained in the invention has the optimum pH value 7. 0 and simultaneously has a high enzymatic activity in a range of pH5-9. The ability for hydrolyzing a phytate phosphorus in a bean pulp of the phytase in a neutral condition is better than the present used phytase, which enables the phytase in the invention to apply to a fresh water fish feed stuff.

The invention discloses a method for preparing an electrochemical biosensor based on a horse radish peroxidase-porous zirconium phytate nanoparticle modified glassy carbon electrode. The method comprises the following steps of: dripping a ZrOCl2 acidic solution into a sodium phytate solution and reacting for 1h; cooling and then centrifuging, and dispersing by using ultrapure water to prepare nano porous zirconium phytate colloid; dripping an appropriate amount of zirconium phytate micelle dispersing solution on the surface of a polished glassy carbon electrode and airing; dripping a horse radish peroxidase solution on a nano zirconium phytate modified glassy carbon electrode, and airing; and dripping an appropriate amount of perfluorinated sulfonic acid solution, and airing to obtain theglassy carbon electrode. The invention has the advantages that: the zirconium phytate colloid dispersing solution with the porous structure is prepared and to be used for fixing biological enzymes, keeps the bioactivity of the enzymes and realizes the direct electron transfer between the enzymes and the electrode; the preparation method is simple; and the response time to the hydrogen peroxide detection is shorter, the detection limit is low, and the repeatability and the stability are good.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and / or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate.

The invention belongs to the technical field of preparation of flexible self-supporting supercapacitors, and discloses a preparation method and application of a self-supporting flexible polyaniline supercapacitor material. The preparation method comprises the following steps: preparing a polyaniline conductive polymer from PVA, phytic acid and triaminobenzeneboronic acid serving as dopants and cross-linking agents through an oxidative polymerization reaction; preparing an electrode; dissolving sulfuric acid in water to prepare a sulfuric acid electrolyte with a concentration of 1 mol / L; and preparing the self-supporting flexible conductive polymer supercapacitor material. According to the invention, a three-dimensional cross-linked conductive polymer micro-nano structure is obtained by utilizing supramolecular doping, and the three-dimensional cross-linked conductive polymer micro-nano structure has good conductivity and specific surface area and has relatively good specific capacity;since PVA and triaminobenzeneboronic acid are added, the flexibility of polyaniline is greatly improved through covalent bonds, and flexibility and self-supporting are achieved; and by adjusting the conductivity and flexible performance, a current collector is not needed in design and preparation, weight is reduced, and the energy density of the whole device is improved.

The invention belongs to the electrochemistry and nano material technical fields, and especially relates to a graphene nano material-modified glassy carbon electrode based on phytic acid dispersion, a preparation method and an application of the electrode in detection of acetaminophen. The preparation method is as follows: graphene is dispersed in a phytic acid solution, and is subjected to ultrasonic treatment for 4-8 h to obtain a phytic acid dispersion liquid of graphene; a clean glassy carbon electrode is soaked in the phytic acid dispersion liquid of graphene, subjected to self-assembly for 1-4 h, washed and dried; the mass concentration of the phytic acid solution is 30-60%. The glassy carbon has advantages of electrode low preparation cost, simple preparation method and environment protection. The glassy carbon electrode can be applied to detection of acetaminophen, and has good electrocatalytic oxidation activity to detection of acetaminophen. The glassy carbon electrode is advantaged by fast response time, wild linearity range, good reproducibility high stability and freedom from interference of coexisting electroactive substances.

The invention, which relates to the technical field of food processing, provides flavored hot pickled mustard tubers and a preparation method thereof. The flavored hot pickled mustard tubers is prepared by using the following raw materials: green vegetable heads, a soaking solution, phytic acid, sodium erythorbate, disodium stannous citrate, citric acid, lactic acid bacteria, sodium alginate, protease and table salt. According to the invention, the flavored hot pickled mustard tubers having crisp and tasty taste, moderate acidity, and unique flavor has extremely low nitrite content and is a nutritional and safe preserved hot pickled mustard tuber product; the sensory quality is improved, the nutritional value is increased, the shelf life is prolonged, so that the healthy consumption concept of modern consumers is met; the lactic acid bacteria are used for pickling the mustard tuber, so that the cost is saved, the food safety of the hot pickled mustard tuber is improved, the flavor of the hot pickled mustard tuber is improved, the quality and functionality of the hot pickled mustard tuber are enhanced. The preparation method is a novel safe and reliable process; production of harmful microorganisms in the preparation process of the hot pickled mustard tuber is reduced, the flavor of the hot pickled mustard tuber is ensured and the shelf life is prolonged.

The invention relates to a height ratio active phytase and the coding gene. The total length of the gene phyQ is 1299bp, and has 432 amino acids comparing to the reporting phytase the most homology is phytase APPA from bacteria coli. The invention also supplies the rebuilding yeast cell containing the phytase gene of the invention. It could be used as the feed additive.