32 results about "P24 antigen" patented technology

The invention discloses a monoclonal antibody hybridoma cell expressing an HIV P24 and an application, wherein, through separating HIV virus in an HIV positive infector, the P24 protein gene of the separated virus is enlarged; P24 protein can be obtained through external expression of carriers and purification; after using the purified P24 protein to immunize the mice, the spleen B cells of the immunized mice are separated out, and an hybridoma cell efficiently expressing a P24 monoclonal antibody (whiov-P24C-MoAb, CCTCC NO:C200818) can be obtained by adopting a hybridoma technology; and the P24 monoclonal antibody can be in specific binding with P24 protein of HIV virus for detecting the expression of P24 antigen. The P24 monoclonal antibody can be in specific binding with P24 protein ofHIV virus, interferences of other bad reactions can be reduced when being used for specific detection of P24 protein.

The invention provides a fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, a preparation method and an application thereof. The test paper comprises a water-absorbing filter paper, a nitrocellulose membrane, a colloidal gold pad, a sample pad and a reaction supporting substance, wherein the sample pad, the colloidal gold pad, the nitrocellulose membrane and the water-absorbing filter paper are overlapped and spliced on the outer surface of the reaction supporting substance in turn. Thus, the fourth-generation HIV antibody antigen test paper provided by the invention has the advantages of convenience in use, simpleness in operation, convenience in popularization and capability of simultaneously identifying P24 antigen positive result and HIV antibody positive result on the same test paper.

The invention relates to a test paper strip for detecting a P24 antigen and particularly relates to a preparation method of a carbon quantum dot test paper strip for detecting a P24 antigen. The preparation method comprises the following steps of: bonding a sample pad and a water absorption pad respectively at two ends of a bottom plate, preparing a P24 carbon quantum dot pad and a P24 nitrocellulose membrane, bonding the sample pad, the P24 carbon quantum dot pad, the P24 nitrocellulose membrane and the water absorption pad in a lapping way in sequence on the bottom plate to obtain a test paper plate, cutting the test paper plate into a plurality of test paper strips conforming to defined widths, and hermetically storing the test paper strips. According to the invention, a known specific antibody or antigen is fixed in a zone of the cellulose membrane at first, a sample to be detected is added on the sample pad, a carbon quantum dot marking reagent on the carbon quantum dot pad is dissolved, and a large amount of carbon quantum dots are gathered on a detection band, so that bright light is emitted and specific immunologic diagnosis is achieved. The method is high in sensitivity and low in toxicity or free from toxicity in a detection process.

The invention discloses an acid treatment agent for HIV detection, a sample pretreatment method, a kit and a detection method, and belongs to the technical field of in vitro diagnosis and detection. The acid treatment agent is mainly composed of an organic acid solution with a concentration of 0.1-1.0mol / L and an inorganic acid solution with a concentration of 0.01-0.1mol / L, or an organic acid solution with a concentration of 0.1-1.2mol / L. The pH value of the acid treatment agent is 2.5-4.5. Using the above-mentioned acidic treatment agent, the p24 antigen and p24 antibody in the sample can be well separated. The HIV detection kit, the sample pretreatment method and the detection method using the above acidic treatment agent have the advantage of high sensitivity.

The invention relates to biology applied technology field, especially relates to a immune chromatography assay for quickly detecting the human immunologic deficiency disease (HIV) antibody and antigen. The assay comprises two independent test papers A and B and plastic case C for storing the test paper, wherein the test paper A is used for detecting the HIV antibody and the test paper B is used for detecting the HIV p24 antigen. The test paper A and test paper B are parallely encased in the plastic case to constitute the assay. On detecting, the detected sample is added on the sample pad of the test paler and the immunoreaction result is directly observed to perform detection. The assay is used for screening or clinical diagnosis of the HIV infection and at the same time the HIV antigen and antibody are detected, the simpler antibody detection can effectively reduce the window phase for detecting the HIV, with features of quick reaction, easy operation, economy and practicality, suitable for insitu detecting.

The invention relates to application of Dalbavancin in preparation of a medicine for curing AIDS, and provides application of Dalbavancin and pharmaceutically acceptable salt thereof in preparation of the medicine for curing AIDS. The experimental result of the anti-HIV activity of Dalbavancin specific to an HIV-1NL4-3 virus strain shows the inhibiting effect of different concentrations of Dalbavancin on the HIV-1NL4-3 virus strain. The EC50 of Dalbavancin that has an inhibiting effect on a syncytium formed through HIV-1 inducing a C8166 cell is 8.694 microns, and the therapeutic index TI is greater than 23.0. A quantitative result in HIV-1p24 antigen shows that the EC50 of Dalbavancin for inhibiting a p24 antigen effect is 1.296 microns, and the therapeutic index TI is greater than 135.1. The experimental result indicates that Dalbavancin has relatively low or no cytotoxicity, possesses remarkable anti-HIV activity and relatively high therapeutic index, and thus can be used for preparing the medicine for curing AIDS.