Diagnostic kit for jointly detecting HIV antigen and HIV antibody and preparation method of diagnostic kit

Abstract

The invention provides a diagnostic kit for jointly detecting an HIV antigen and an HIV antibody. The HIV-1type p24 antigen and the HIV-1 / 2 type antibody are qualitatively detected by adopting the immunoadsorption test principle of a double antibody sandwich method and a double antigen sandwich method and combining a time-resolved fluorescence immunoassay technique; the window period of existing HIV detection can be shortened to be 2-3 weeks, and the diagnostic kit is beneficial for early diagnosis of an HIV and also can serve as a basis for prognostically judging and evaluating the antiviral therapy effect; meanwhile, the kit further has the advantages of being high in specificity and sensitivity, good in repeatability, excellent in stability, wide in measurable range, high in detection automation degree, free of environmental pollution and the like.

Description

technical field [0001] The invention relates to the technical field of virus protein immune analysis, in particular to a diagnostic kit for combined detection of HIV antigen and antibody and a preparation method thereof. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a lentivirus that infects cells of the human immune system. This virus destroys the immune ability of human body, causes immune system to lose resistance, develops to the last, causes AIDS (acquired immunodeficiency syndrome). [0003] So far, there are no effective anti-HIV drugs and HIV vaccines for prevention. Therefore, screening and controlling the source of infection and cutting off the HIV transmission route are important means to prevent HIV transmission and infection. In the early stage of HIV infection, the HIV core antigen p24 first appears in the blood of infected persons, and the level of p24 develops with the development of viral RNA level, and ca...

AI Technical Summary

Problems solved by technology

The advantage of the nucleic acid PCR method is that it has high sensitivity and specificity, but the disadvantages are also relatively obvious, that is, the detection needs to be equipped with more complex instruments, and the operators need to be trained, resulting in high detection costs and complicated operations.

The advantage of ELISA method is that it is easy to operate, does not require complicated instruments, and only needs simple training to operate. However, due to the limitation of the platform, its sensitivity is relatively low, the measurement range is relatively narrow, and the general detection window period is relatively low. longer

Chemiluminescent CLIA method has the advantages of high sensitivity, specificity and wide detection range; the disadvantage is that most plate-type CLIA kits still use the same enzymatic reaction as EIA, using horseradish enzyme or alkaline phosphatase to cross-link antibodies. Luminol or adamantane, etc. are used as substrates to detect dynamic luminescence, resulting in rapid attenuation of the detection signal value and inconsistent signal attenuation speeds between high and low concentrations, which limits its low-end detection sensitivity and stability in immunoquantitative analysis. sex and repeatability

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