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Acid treating agent for HIV detection, sample pretreatment method, kit and detection method

A technology for detection kits and treatment agents, which is applied in the field of acid treatment agents for HIV detection, can solve problems such as missed or misdiagnosed, low antigen content, false positives, etc., and achieve the effect of improving detection sensitivity

Active Publication Date: 2015-06-10
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But even when the virus is actively replicating, the amount of antigen in the serum rarely reaches this level
Moreover, when routinely using chemiluminescent immunoassay to detect HIV, false negatives and false positives often occur, resulting in inaccurate HIV diagnosis results, missed diagnosis or misdiagnosis, and the consequences are very serious

Method used

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  • Acid treating agent for HIV detection, sample pretreatment method, kit and detection method
  • Acid treating agent for HIV detection, sample pretreatment method, kit and detection method
  • Acid treating agent for HIV detection, sample pretreatment method, kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] A HIV detection kit, comprising the following components:

[0079] 1) Acid treatment agent:

[0080] Acetic acid solution with a concentration of 0.5mol / L and hydrochloric acid solution with a concentration of 0.05mol / L are mixed according to the molar ratio of acetic acid and hydrochloric acid of 10:1; and 0.05mol / L acetate is added at a volume ratio of 1:1 buffer solution to obtain an acidic treatment agent, the pH of which is 3.5.

[0081] 2) Magnetic microsphere system: magnetic microsphere solution coated with HIV 1+2 recombinant antigen 1, wherein: the working concentration of HIV 1+2 recombinant antigen 1 is 10 μg / mL, and the working concentration of magnetic microspheres is 0.5 mg / mL.

[0082] A magnetic microsphere solution coated with anti-HIV p24 monoclonal antibody A, wherein: the concentration of anti-HIV p24 monoclonal antibody A is 10 μg / mL, and the working concentration of the magnetic microspheres is 0.5 mg / mL.

[0083] 3) Marker system: ABEI-labele...

Embodiment 2

[0105] A HIV detection kit, which is basically the same as the detection kit in Example 1, except that:

[0106] 1) Acidic treatment agent: mainly composed of 0.1mol / L acetic acid solution and 0.01mol / L hydrochloric acid solution, according to the molar ratio of acetic acid and hydrochloric acid is 20:1, the pH of the acidic treatment agent The value is 3.5.

[0107] A HIV detection method is basically the same as the detection method in Example 1, except that the detection kit of this embodiment is used for detection.

Embodiment 3

[0109] A HIV detection kit, which is basically the same as the detection kit in Example 1, except that:

[0110] 1) Acidic treatment agent: mainly composed of 0.5mol / L acetic acid solution and 0.05mol / L hydrochloric acid solution, according to the molar ratio of acetic acid and hydrochloric acid is 1:1, the pH of the acidic treatment agent The value is 3.5.

[0111] A HIV detection method is basically the same as the detection method in Example 1, except that the detection kit of this embodiment is used for detection.

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Abstract

The invention discloses an acid treating agent for HIV detection, a sample pretreatment method, a kit and a detection method, and belongs to the technical field of in vitro diagnosis and detection. The acid treating agent is mainly formed by mixing an organic acid solution with concentration of 0.1-1.0mol / L and an inorganic acid solution with concentration of 0.01-0.1mol / L, or an organic acid solution which is with concentration of 0.1-1.2mol / L, wherein the pH value of the acid treating agent is 2.5-4.5. The acid treating agent can well separate out p24 antigen and p24 antibody in a sample. The invention also discloses the HIV detection kit adopting the acid treating agent, the sample pretreatment method and the detection method, and the HIV detection kit, the sample pretreatment method and the detection method have the advantage of high sensitivity.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis and detection, in particular to an acid treatment agent for HIV (human immunodeficiency virus) detection, a sample pretreatment method, a kit and a detection method. Background technique [0002] After the HIV virus (human immunodeficiency virus) invades the human body, its surface glycoprotein gp120 binds to the cell surface receptor protein CD4 with high affinity and adsorbs to the host cell; gp120 interacts with the host cell surface co-receptor to make the virus and The host cell membrane is closer; gp41 produces a series of conformational changes, and its N-terminal fusion peptide fragment is inserted into the host cell membrane, resulting in the final fusion of the viral envelope and the cell membrane, and viral RNA enters the cell. After HIV infection, viral RNA can be detected first, then the viral p24 antigen, and finally antibodies. According to the characteristics of HIV gen...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/571
Inventor 饶微袁锦云方中刚刘晓森罗凯李婷华
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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