Monoclonal antibody hybridoma cell of HIV P24 and application

Abstract

The invention discloses a monoclonal antibody hybridoma cell expressing an HIV P24 and an application, wherein, through separating HIV virus in an HIV positive infector, the P24 protein gene of the separated virus is enlarged; P24 protein can be obtained through external expression of carriers and purification; after using the purified P24 protein to immunize the mice, the spleen B cells of the immunized mice are separated out, and an hybridoma cell efficiently expressing a P24 monoclonal antibody (whiov-P24C-MoAb, CCTCC NO:C200818) can be obtained by adopting a hybridoma technology; and the P24 monoclonal antibody can be in specific binding with P24 protein of HIV virus for detecting the expression of P24 antigen. The P24 monoclonal antibody can be in specific binding with P24 protein ofHIV virus, interferences of other bad reactions can be reduced when being used for specific detection of P24 protein.

Description

A kind of HIV P24 monoclonal antibody hybridoma cell and application technical field The present invention relates to the technical field of HIV (HIV) detection, more specifically to a hybridoma cell expressing HIV P24 protein monoclonal antibody (abbreviated as monoclonal antibody), and also relates to a kind of HIV-1P24 monoclonal antibody in the detection Application in the ELISA detection method of P24 protein (for detecting the method of enzyme-linked immunosorbent assay (Enzyme-LinkedImmunoSorbentAssay, ELISA) of P24 protein antigen) Background technique Acquired immunodeficiency syndrome, referred to as AIDS (AIDS), is a serious infectious disease caused by human immunodeficiency virus (humanimmunodeficiencyvirus, HIV) infection. Since the first AIDS patient was discovered in China in 1985, the epidemic has been accelerating, and the epidemic is spreading from high-risk groups to the general population. Since there is no effective preventive vaccine or cure for AID...

AI Technical Summary

Problems solved by technology

Due to the high cost of imported reagents, the widespread use of more domestic laboratories is limited, which is not conducive to the development of relevant scientific research in China

In addition, the P24 monoclonal antibodies prepared by foreign manufacturers all use the P24 antigen of the European and American strains as the binding target, which may be difficult to meet the needs of domestic AIDS research in scientific research

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