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Fluorescent nanoparticles Ru(bpy)3/SiO2, preparation method and application thereof

A fluorescent nanometer and particle technology, used in chemical instruments and methods, luminescent materials, material inspection products, etc.

Inactive Publication Date: 2010-10-20
SHANGHAI UNIV
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Problems solved by technology

However, the use of nanoparticles as markers in the detection of HIV on protein microarray chips has not been reported in the literature.

Method used

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  • Fluorescent nanoparticles Ru(bpy)3/SiO2, preparation method and application thereof
  • Fluorescent nanoparticles Ru(bpy)3/SiO2, preparation method and application thereof
  • Fluorescent nanoparticles Ru(bpy)3/SiO2, preparation method and application thereof

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Embodiment Construction

[0021] 1. Instruments and reagents: Hitachi F-7000 fluorescence spectrophotometer (Hitachi, Japan); Hitachi U-3010 ultraviolet-visible spectrophotometer (Hitachi, Japan); Hitachi CR22G / II high-speed centrifuge (Hitachi, Japan); JEOL 200CX Transmission Electron Microscope (JEOL Ltd.). Genepix 4000B chip scanner (Axon Instruments, USA); OminiGrid 300 chip pointing instrument (Genomics Solution, USA).

[0022] Ruthenium terpyridine (Rubpy) 3 , 3-aminopropyltrimethoxysilane (APTMS), tetraethyl orthosilicate (TEOS), TritonX-100, etc. are all analytically pure, purchased from SIGMA Company; n-hexanol, acetone, ammonia water, etc. are all analytically pure, purchased from From Shanghai Haoshen Chemical Reagent Co., Ltd.; bovine serum albumin (BSA), streptavidin (SA), etc. were purchased from Beijing Xinjingke Biotechnology Co., Ltd. HIV p24 antigen, biotin-goat anti-HIV p24 polyclonal antibody was purchased from Fitzgerald Company in the United States, goat anti-HIV p24 monoclonal ...

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Abstract

The invention relates to fluorescent nanoparticles Ru(bpy)3 / SiO2, a preparation method and application thereof. The fluorescent nanoparticles have nuclear shell structures; the nuclear shell structure is formed by taking tris(2,2'-bipyridyl)ruthenium as a core, covering silicon dioxide with netlike structure on the surface of the tris(2,2'-bipyridyl)ruthenium and carrying active amino groups on the surface of the silicon dioxide, wherein the mass ratio of the tris(2,2'-bipyridyl)ruthenium to the silicon dioxide is 1:5 to 1:8; and every milligram of nanoparticles comprises 385nmol of amino group. The silicon fluorescent nanoparticles Ru(bpy)3 / SiO2 have the advantages of uniform size, high monodispersity, mean diameter of 70+ / -6nm, high light stability and difficult dye leakage in aqueous solution. The fluorescent probe is applied to a protein microarray chip to detect HIV p24 antigen after marking streptavidin; the analysis method is a sandwich fluorescence immunoassay method; and the result shows that the fluorescence intensity is in good positive relationship with p24 concentration and the analytical sensitivity is 3.1ng / mL. The result shows that the nanoparticles, serving as a novel fluorescent probe, can be applied to the systems of the protein microarray chip and fluorescence immunoassay and the like for high flexibility detection.

Description

technical field [0001] The invention relates to a fluorescent nanoparticle Ru(bpy) 3 / SiO 2 , its preparation method and application. Background technique [0002] AIDS is a serious infectious disease caused by human immunodeficiency virus infection. As there is currently no vaccine for the prevention of AIDS, timely and correct diagnosis of HIV infection is an important means to treat and reduce the spread of AIDS. At present, the commonly used routine diagnosis method for AIDS is the ELISA method, but the ELISA method is generally carried out on a 96-well plate, which has the disadvantages of large reagent consumption, high detection cost, long detection time, insufficient detection sensitivity and high-throughput quantification. It is difficult to achieve high-throughput, high-sensitivity, and rapid detection of a large number of groups, such as HIV census and monitoring of people in areas with severe epidemics, and screening of blood donations. [0003] Protein micro...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/02G01N33/52
Inventor 尹东光刘斌虎张礼谢春娟张乐
Owner SHANGHAI UNIV
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