113 results about "Bacteriome" patented technology

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

Disclosed herein are fire-fighting compositions, and methods thereof, comprising a surfactant and a fraction of a fermentation mixture comprised of yeast exoproteins, where the proteins enhance ability of water to extinguish Class A, Class B and Class K fires, as defined by the National Fire Protection Association. In one embodiment the surfactant is optimized to wet and penetrate into the substrate, for example wood, as in a Class A fire. In a second embodiment, the surfactant is optimized for foaming to provide fire fighting protection in a Class B fire. Further embodiments include methods for using the same protein-surfactant fire fighting composition for both polar (e.g., alcohol) and non-polar combustible liquids. In yet another embodiment, methods are described where the proteins comprise stress shock proteins and where the residual protein-surfactant combination that is not degraded from the fire acts to stimulate resident bacterial populations to accelerate the biodegradation of residual hydrocarbons and surfactants from the composition. In another embodiment, the compositions are free of solvents, fluorine-free as in fluoro surfactants and fluoro-polymers.

The invention discloses an automatic method for analyzing constitution and diversity of a bacterial community of a 16SrRNA gene. The automatic method comprises the following steps: in 16SrRNA sequencing data analysis process, by taking original sequencing sequence data as input, calling a standard analysis tool (such as Mothur and QIIME) of the industry, and performing visualization on data finally, thereby obtaining analysis results which are easy to analyze. The automatic method includes popular main analysis items at present, meanwhile analysis contents can be modularized, relatively rich and deep data mining analysis methods can be available, different analysis module contents can be combined according to different demands, and process procedures can be relatively reasonably arranged; and in addition, analysis errors caused by different sequencing depths can be eliminated, and the analysis results can be relatively comprehensive, accurate and reliable.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The present invention relates to nucleic acid molecules which allow the identification of bacteria or groups of bacteria. For detection, the region of the bacterial genome containing the 23 S / 5 S rRNA is used as the target sequence for the bacterial detection.

The invention discloses a cell-based biosensor used for detection of bacterial-colony signal molecules and a preparation method of the cell-based biosensor. Graphene oxide is added to a sodium alginate solution to form a sodium alginate / graphene oxide mixture, a cell suspension solution is inoculated to the surface of a gold electrode, cells are covered and wrapped with the sodium alginate / graphene oxide mixture and are cultivated and inoculated after soaked in a CaCl2 insulation to form hydrogel to obtain the cell-based biosensor; the constructed cell-based biosensor is applied to detection of 3OC12-HSL of N-acyl-homoserine lactone (AHLs) standard substances in the bacterial-colony signal molecules, good linear relation of the concentration at 0.01mu M to 1um M is achieved, and detection limit is 0.01um M. Identification, conduction and detection on the standard substances of the colony signal molecules are realized accurately, efficiently and rapidly, shortcomings in traditional detection methods are overcome, and the cell-based biosensor is simple in manufacture, low in cost and good in application prospect.

Compounds which modulate quorum sensing in quorum sensing bacteria. Compounds of the invention inhibit quorum sensing and / or activate quorum sensing in various bacteria. Compounds that inhibit quorum sensing are particularly useful for inhibition of detrimental bacterial biofilm formation. Compounds that activate quorum sensing are particularly useful for promoting growth and biofilm formation of beneficial bacterial.

The invention relates to antibacterial application of small molecular bacterial quorum sensing inhibitor Falcarindiol in the condition of no inhibition on bacteria growth concentration (subinhibitory concentration). The active compound has a broad-spectrum bacterial quorum sensing resistant activity, can inhibit bacterial virulence in subinhibitory concentration, reduces the harm of bacteria to the host, and can prevent and treat quorum sensing bacterial infections. The compound can play the role of inhibition of bacterial virulence in subinhibitory concentration, so that pressure for survival of pathogenic bacteria is not brought, drug resistance is not easy to produce, the antibacterial application of the small molecular bacterial quorum sensing inhibitor Falcarindiol in the condition of no inhibition on bacteria growth concentration (subinhibitory concentration) is different from application of killing bacteria and bacteria growth inhibition of other antibacterial agent, is also is different from the antibacterial application of bacteria growth inhibition of Falcarindiol with the bacteria concentration (the use final concentration is greater than the minimum bacteriostasis concentration), and is a new antibacterial application. The antibacterial application of the small molecular bacterial quorum sensing inhibitor Falcarindiol in the condition of no inhibition on bacteria growth concentration (subinhibitory concentration) has the advantages that: raw materials are easy to get, compound separation and purification are simple and easy to control, security is high, use concentration is low, and drug resistance is easy to produce, and the like, so that the Falcarindiol can be used in preparation of a new antibacterial agent.

The present invention is directed to microbiological processes for the oxidative treatment of refractory gold and base metal ores and concentrates, and particularly, an apparatus for testing refractory gold and sulfidic base metal ores for their suitability to heap biooxidation or bioleaching processes. The invention can be used to simulate heap biooxidation. This apparatus incorporates features of heap biooxidation and may be used to generate engineering data for creating detailed process and engineering designs. The invention can be used to develop a select population of bacteria geared to optimize biooxidation of a specific refractory ore.

The invention provides a method for rapidly screening a bacterial quorum sensing inhibitor (QSI) by utilizing a suicide gene. The method is characterized in that under natural light, bacteria not infected with a phage do not turn blue and bacteria infected with the phage turn blue; induced by an added lethal condition inducer, a specific polypeptide with the function of inhibiting QS does not start expression of the suicide gene because of inhibiting the activities of QS molecules, so the bacteria survive; a non-specific polypeptide can not inhibit the QS molecules, so the suicide gene starts lethal; then survival candidate clones are picked out, functional verification is further carried out and finally a DNA (deoxyribonucleic acid) sequence corresponding to a specific polypeptide sequence is obtained by a method of DNA sequencing, thus obtaining an efficient and specific QSI polypeptide. The method is simple in steps, and multiple QSI candidate polypeptides can be obtained only within three days to about one weak, so that the method has very obvious advantages.

Disclosed is a food or a drug whereby the composition ratio of intestinal bacterial flora can be safely regulated with little side effects. An agent for regulating the composition ratio of intestinal bacterial flora characterized by containing at least one kind of sugar absorption inhibitor, and a food or a drug containing the same.

The invention discloses a use of hyperoside in preparation of a drug for inhibiting a bacterial quorum sensing system. Hyperoside can inhibit a quorum sensing signal molecule thereby reducing bacterial biomembrane formation and virulence factor pathogenicity and reducing bacterial swarming motility so that bacterial resistance to drugs and bacterial pathogenicity are reduced. The hyperoside has low toxicity and use safety.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention provides a closed-loop type garbage digesting and saline-alkali soil sandy land repairing and garbage dump in-site repairing method. The method comprises the steps that garbage is classified firstly; micropopulation in the bovine rumen is added, fiber in garbage is rapidly decayed, and starch type garbage is rapidly decomposed; after rapid decomposition and decaying, the mixed thermophile colony for high-temperature degrading of starch and proteins and decomposing of wood fiber is added for continuous fermentation, and the lactic acid bacteria colony is added for metal removal. After desiccation, a soil repairing agent made of plant ash is added; the salt and alkali sandy soil is treated; and the prepared soil repairing agent is sufficiently mixed with soil, and soil is repaired. According to the method, a pollution source can be eradicated from the source, and the garbage is turned into wealth and is beneficial to returning to the nature.

A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.

The invention discloses application of pyrimidine derivative in preparing medicine for inhibiting a bacterial quorum sensing system. According to the application disclosed by the invention, LasR protein is utilized as target protein to perform virtual screening to obtain the pyrimidine derivative with a higher score, and the pyrimidine derivative is utilized to perform biological activity evaluation on pseudomonas aeruginosa. Experiments prove that the pyrimidine derivative shows down-regulation on three kinds of genes of LasR, LasB and LasI related to the LasR in culture. Effects of the pyrimidine derivative with the higher score in screening results and the target protein are shown to have an inhibition effect on expression of downstream related genes. Namely, the pyrimidine derivative can inhibit quorum sensing signal molecules to finally reduce bacterial biofilm formation and effectively inhibit pathogenicity of virulence factors and quorum sensing of bacteria. Computer virtual screening is a compound screening method with high efficiency and low cost, so that feasibility for application of the technology in studying quorum sensing medicine is achieved.

The invention provides a wilt disease prediction model based on high-throughput sequencing data and application. The method comprises the following steps: integrating microorganism high-throughput sequencing data of wilt disease soil and healthy soil of different regions and different crops; analyzing the characteristics of bacterial communities and fungal communities in the diseased soil and thehealthy soil by using a machine learning method; obtaining relative abundance characteristics of classification operation units of 45 bacteria and 40 fungi and using the characteristics for distinguishing the healthy soil from wilt disease soil, and establishing a bacterium model and a fungus model respectively. The disease condition of soil wilt is accurately distinguished, and microflora characteristics of wilt disease soil are revealed.

The present invention provides methods of preventing or inhibiting biofilm formation by a population of bacteria, said method comprising the administration to the population of an antibody to a lactone or lactone-derived signal molecule secreted by bacteria. The invention therefore also provides methods for the treatment of bacterial infection in biofilm formation is prevented or inhibited.

The invention provides a pure natural plant acne-removing and mark-fading composition, essence containing the composition and a preparation method and application of the essence. The pure natural plant acne removing and mark fading composition is prepared from the following components in parts by weight: 0.1 to 5 parts of white willow bark extract, 0.1 to 10 parts of guava fruit extract, 0.1 to 10parts of pinus densiflora leaf extract, 0.1 to 10 parts of lactobacillus fermentation product and 1 to 5 parts of madecassoside. The invention aims to solve the problems of acne and the like easily caused by large oil yield of skin of men. According to the invention, the concept of micro-ecological acne removal is combined; the white willow bark extract, the guava fruit extract, the pinus densiflora leaf extract, the lactobacillus fermentation product and the madecassoside play a synergistic effect according to a reasonable proportion, the effect of an anti-bacterial colony effect is achieved, the number of propionibacterium acnes is balanced, meanwhile, the effects of inhibiting bacteria, controlling oil, relieving inflammation, promoting crevasse healing, repairing acne marks and promoting cuticle metabolism can be achieved, and the purposes of scientific acne removal and long-term acne prevention are achieved.

The invention discloses a domestication method of an ammonia nitrogen and propionic acid resisting methane production strain. The ammonia nitrogen and propionic acid resisting methane production strain comprises a propionic acid oxidization flora, a syntrophaceae propionic acid oxidization flora, an acetic acid cracking methane production flora, a hydrogen nutrition type methane production flora and a denitrification flora. The method takes marsh gas fermentation liquid as an inoculum, takes propionic acid as a carbon source and takes urea or NH4Cl as an ammonia nitrogen source; the marsh gas fermentation liquid is domesticated through a method for alternately improving the concentration of the propionic acid and the concentration of ammonia nitrogen, so as to obtain the ammonia nitrogen and propionic acid resisting methane production strain; the strain can stably produce gas for a long period under the conditions that the concentration of the ammonia nitrogen is more than 3.0g / L and the concentration of the propionic acid is more than 2.0g / L; the gas yield is 60 percent more than a theoretical yield; the utilization rate of the propionic acid and acetic acid is more than 90 percent; the ammonia nitrogen and propionic acid resisting methane production strain has the advantages of high impact resistance, high stability of biological enhancing effect and the like, and can be used for removing comprehensive inhibition of the ammonia nitrogen and the propionic acid in a marsh gas fermentation process and improving the fermentation performance of marsh gas.

The disclosed invention is directed to an implantable surgical prosthetic mesh having a nanofiber comprising one or more antibiotic and a polysaccharide, and a non-polysaccharide polymer deposited on the mesh. The mesh of the invention is shown to be effective in eliminating or minimizing the bacterial population in the mesh and surrounding tissue for at least 14 days from surgical implantation of the mesh.

The invention relates to three bacteria Angiococcus and a method for screening and purifying Angiococcus. Rabbit excrement is used as raw material for screening, and an antifungal is used to inhibit the growth of fungi such as mold and so on. Flat screening, streaking, dilution and purification, low-temperature freezing and thawing, heat treatment and so on are adopted in turn for screening and purification. A calcium chloride water culture medium which is crossways streaked by microzyme and colibacillus (living or dead fungi) is further screened to get three bacteria through separation. The three bacteria belong to Angiococcus according to the characteristics of mycology. According to Bergey Manual of Determinative Bacteriology (the ninth edition), the three bacteria belong to three new bacterial strains.

The invention relates to a cellulose degradation composite flora which is composed of bacterial species and fungal species. The composite flora horizontal bacterial community is mainly composed of fontibacillus, stenotrophomonas, paenibacillus and pantoea; and the fungal community is mainly composed of petromyces and nectria. The composite flora is obtained by adopting a subculture continuous culture method; wheat straw is added into a Mandels culture medium to serve as a unique carbon source and is obtained by screening litters under a perennial pinus sylvestris forest in a Maowusu sandy land; and the composite flora can be used for innocent treatment and resource utilization of agricultural wastes such as phellinusribis bran. The method overcomes the defects of low efficiency, poor constant value, weak competitive capacity with indigenous microorganisms, non-ideal practical application effect and the like of cellulose degrading bacteria in practical application in the prior art, is suitable for decomposing and composting phellinusribis bran which is difficult to degrade, and can quickly and efficiently realize harmless treatment and resource utilization of the agricultural wastesdifficult to decompose.

The present invention provides a method which can enhance the precision of identification of bacterial species using a T-RFLP method and achieve, by itself, the identification of bacteria constituting a bacterial flora and the tracing of distribution changes thereof. The present invention provides a method for analyzing a bacterial community including: amplifying DNAs extracted from a bacterial community by PCR using 16S rRNA genes as templates and fluorescently labeled primers; cleaving the amplification products with a restriction enzyme to thereby obtain sample PCR fragments; electrophoresing the sample PCR fragments together with size standard PCR fragments; and comparing the mobilities thereof to thereby determine the sizes of the sample PCR fragments, wherein PCR fragments amplified by using, as a template, a 16S rRNA gene derived from a bacterium contained in the bacterial community to be analyzed are used as the size standards.

The invention discloses a biological enzyme water purifying agent. The biological enzyme water purifying agent is characterized by comprising, by weight, 10%-15% of protease, 10%-15% of lipase, 10%-15% of cellulose, 10%-15% of pectinase, 5%-15% of bacillus brevis, 5%-10% of nitration coccus, 0.1%-2% of rosa multiflora extracting solution, and the balance being water being completed to 100%. The biological enzyme water purifying agent is a completely natural biological product. Use of the biological enzyme water purifying agent is free of influence of creature types (such as fishes, shellfishes, mollusks, and plankton) existing in water. The biological enzyme water purifying agent not only cannot cause damage to the aquatic organisms, but also strengthens activity of aquatic organism groups due to the improvement of water environment, can purify the water environment, increases transparency and dissolved oxygen of a water body, largely decreases ammonia nitrogen, biochemical oxygen demand (BOD) 5, and phosphorus, balances saprophytic bacteria community, decreases pathogenic bacteria and native parasites, can remove nutriment such as nitrogen and phosphorus in water, enable algae to lose a growing base so as to retrain growth of the algae, and the function of natural self-purification is recovered.

The invention provides a sludge soil restoration agent, a preparation method, and application of the sludge soil restoration agent in sludge digestion. The invention adopts the technical scheme that municipal sewage sludge is treated at an early stage, an organic matter is decomposed by thermophilic bacteria, and homonymous bacteria colonies are inoculated. Through an experiment, after the sludge soil restoration agent is used for 20 days, the proportional effective removal rate of various pollutants of soil achieves 95 percent or above so as to meet the national standard. Meanwhile, a sulfuric acid matter can acidize a hardened salt and alkali land, is good organic fertilizer, is beneficial for reducing the use of a chemical reagent and chemical fertilizer in agricultural production, and completely eradicates a pollution source and secondary pollution from the source.

The present invention relates to a vector, preferably included in a delivery vehicle, comprising no more than (100), preferably no more than (10), restriction sites recognized by the restriction enzymes encoded by each bacterium of a group of bacteria of interest. The invention also relates to the use of said vector, preferably included in a delivery vehicle, as a drug, especially in the treatment of a disease in a patient in need thereof.

A person's intestinal (gut), oral or skin microbiota is modified using specific combinations of pre-biotics, pro-biotics and / or anti-biotics to establish a defined microbiota that can treat and / or reduce the likelihood that individuals will experience various diseases, including cancer. The employment of various bacteria, whether in particular combinations or after being modified using CRISPR-type systems, leads to improved outcomes when checkpoint inhibitors are used to treat various forms of cancer. One embodiment is directed to a method for reducing the likelihood of developing cancer by providing in the gut of an individual a population of beneficial bacteria selected from the group consisting of Lactobacillus species. The level of Roseburia and Faecalibacterium prausnitzii, and / or Akkermansia muciniphila bacteria are increased in the individual's gut microbiome such that when an individual is administered an immune checkpoint inhibitor, its function is enhanced due to the presence of the bacterial population.

The invention belongs to the technical field of medicines, and particularly relates to application of a compound in preparation of a medicine for inhibiting a bacterial quorum sensing system. The invention provides application of a compound in preparation of a medicine for inhibiting a bacterial quorum sensing system. Experiment results show that the 2-(3', 4'-dihydroxyphenyl)-1, 3-piperidine-5-aldehyde can inhibit three signal paths of a Pseudomonas aeruginosa quorum sensing system pqsA, lasB and rhlA and inhibit the activity of the Pseudomonas aeruginosa quorum sensing system without inhibiting the growth of the Pseudomonas aeruginosa. Therefore, the pathogenicity and toxicity of the bacteria are reduced without inhibiting the growth of the bacteria, and the generation of multiple drug resistance of the bacteria is slowed down.