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Three-strain balloon slime bacteria and sifting motion purification process

A technology of myxobacteria and cysts, which is applied in the field of three strains of Myxobacteria and their screening and purification, and can solve the problems of time-consuming cultivation and restricting Myxobacteria.

Inactive Publication Date: 2008-09-24
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the time-consuming isolation, purification and cultivation of myxobacteria restricts the research on myxobacteria, resulting in only a few laboratories in the world studying myxobacteria

Method used

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  • Three-strain balloon slime bacteria and sifting motion purification process

Examples

Experimental program
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example 1

[0018] Example 1: 1. Plate screening: get an appropriate amount of rabbit feces, soak it for 6 hours with an antifungal drug (carbendazim 30mg / l), then inoculate it in an Angel yeast water medium containing an antifungal drug (carbendazim 30mg / l) (Angelica yeast 0.2%, agar powder 1.5%, pH 7.2) plates, placed in a 30°C incubator for 4 days of constant temperature and humidity culture, picked the colonies suspected of myxobacteria in time, and transferred them to VY / 4 medium (vitamin yeast medium / 4: Angel Yeast 0.25%, CaCl 2 2H 2 O 0.1%, Vitamin B 12 0.5 μg / mL, agar powder 1.5%, pH 7.2) on the plate, and continue to culture at 30 ° C for 4 days.

[0019] 2. Streak dilution and purification: Pick the bacteria on the edge of the suspected colony from the VY / 4 plate, streak on the VY / 4 medium plate, and culture at 30°C for 4 days.

[0020] 3. Low-temperature freeze-thaw treatment: Inoculate the single colony obtained in step 2 on the VY / 4 slant medium, freeze in a -80°C refrige...

example 2

[0024] Example 2: Screening method is identical with example 1, and antifungal drug is griseofulvin 60mg / l, and constant temperature and moisture are cultivated for 4 days, and during further screening, live Escherichia coli is cross-marked on calcium chloride water medium. The pure strain is obtained by screening, and the preservation number is: CGMCC1960.

example 3

[0025] Example 3: Screening method is identical with example 1, and antifungal drug is clotrimazole 60mg / l, and constant temperature and moisture are cultivated for 3 days, cross-mark on calcium chloride water medium with live yeast during further screening. The pure strain is obtained by screening, and the preservation number is: CGMCC1961.

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Abstract

The invention relates to three bacteria Angiococcus and a method for screening and purifying Angiococcus. Rabbit excrement is used as raw material for screening, and an antifungal is used to inhibit the growth of fungi such as mold and so on. Flat screening, streaking, dilution and purification, low-temperature freezing and thawing, heat treatment and so on are adopted in turn for screening and purification. A calcium chloride water culture medium which is crossways streaked by microzyme and colibacillus (living or dead fungi) is further screened to get three bacteria through separation. The three bacteria belong to Angiococcus according to the characteristics of mycology. According to Bergey Manual of Determinative Bacteriology (the ninth edition), the three bacteria belong to three new bacterial strains.

Description

technical field [0001] The invention relates to three strains of Angiococcus sp. and a screening and purifying method thereof. Background technique [0002] Myxobacteria, the highest group of prokaryotes, are gram-negative bacteria with complex multicellular behavior that move in a sliding manner. Myxobacteria not only play an important role in the study of cell differentiation, development and biological evolution, but also can produce abundant secondary metabolites, and the rate of positive bacteria producing antibacterial activity is even higher than that of the famous secondary metabolite producing bacteria Actin Myxobacteria have so far been found to produce hundreds of structurally different compounds, many of which had never been discovered before. Therefore, myxobacteria are a microbial group with great research and development value. However, at present, the time-consuming isolation, purification and culture of myxobacteria restricts the research on myxobacteria, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 余蓉李晓红刘冰花熊世娟卞国宁杨继虞
Owner SICHUAN UNIV
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