43 results about "Achromobacter sp" patented technology

The invention provides novel achromobacter sp. D-12 for efficiently degrading acetochlor of amide herbicides and application of the novel achromobacter sp. D-12. The strain was preserved in the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China on September, 27th, 2012, and the preservation number is CCTCC No: M2012386. The achromobacter sp. D-12 can be applied to the degradation of the acetochlor in a water body in a direct adding mode, and can be used for safely, efficiently and quickly degrading the acetochlor remained in the water; and a microbial inoculum containing the strain is easy to prepare, low in cost and convenient to use, and has a good application prospect.

The invention aims at developing a microbial method for processing gasoline petroleum hydrocarbons, and provides a microbial agent for degrading petroleum hydrocarbons and a using method of the microbial agent. The microbial agent is composed of Micrococcus sp., Pandoraea sp. and Achromobacter sp., preferably, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. in the microbial agent is (8-16): (5-12): (10-16), or, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. is 4:4:5; or, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. is 2:1:2. According to the using method of the microbial agent for degrading the petroleum hydrocarbons, the microbial agent is used for degrading gasoline petroleum hydrocarbons, wastewater containing the gasoline petroleum hydrocarbons, or soil containing the gasoline petroleum hydrocarbons. After being treated by the microbial agent, the gasoline petroleum hydrocarbons can be rapidly and completely degraded, and thus a new idea is provided for solving the environmental pollution problem caused by gasoline or other petroleum hydrocarbons.

The invention discloses a microbial leaching and chemical fixing joint repairing method for heavily polluted soil of a chromium slag yard. The method comprises the following steps: spraying soil or muck of a chromium slag yard with a composite bacteria liquid which is obtained by cultivating compound strains Pannonibacter phragmitetus BB and Achromobacter sp.Ch<-1>; restoring Cr(VI) in the obtained leacheate into Cr(III), forming sediments in an alkaline condition, precipitating and recycling chromium; and carrying out chemical fixing on the Cr(VI) which is not leached from the soil or muck through hydrazine hydrate. By virtue of the method, the soil and muck with three different Cr(VI) pollution levels are repaired; the total removal rates on chromium are respectively 11.4%, 30.2% and 14.0%; the removal rates on water-soluble Cr(VI) are respectively 99.6%, 99.5% and 98.3%; and the leaching toxicity concentration of the Cr(VI) in the soil or muck is lower than 0.5mg/L.

The invention discloses achromobacter sp. and an application thereof to improvement of iron nutrition of peanuts. The achromobacter sp. is named as 1604IPR-02, and the preservation number is CGMCC NO.16955. The achromobacter sp. 1604IPR-02 is a gram-negative bacterium, does not have spores, has high siderophore production capacity, can convert ferrum which is slightly soluble in soil into effective ferrum, can increase soil nutrients, can promote absorption of the ferrum in the soil by plants, particularly can be successfully colonized in peanut rhizosphere soil, can promote growth of the peanuts, can increase yield, and has excellent application potential.

The invention relates to the technical field of biodegradation treatment, and aims at providing a preparation method and an application of a grease degrading bacterium community. The preparation method of the grease degrading bacterium community comprises the following steps: adding a deposit sample and grease collected from a food waste hogwash grease wastewater zone to an inorganic salt medium, and culturing the deposit sample and grease at 30DEG C under a rotating speed of 150r/min for 5d; repeatedly culturing the obtained material through the same technology 2 times to obtain a final liquid which is a bacterial liquid containing the grease degrading bacterium community; and carrying out DNA sequencing confirmation, wherein the grease degrading bacterium community comprises Stenotrophomonas maltophilia, Chryseobacterium spp, Achromobacter sp and enterobacteria. Onsite sampling (for achieving low cost) is realized, the grease degrading bacterium community is enriched and screened through a simple and convenient process, and grease wastewater in the food waste hogwash grease wastewater zone is effectively degraded; and the degradation efficiency in the treatment of the grease wastewater in the food waste hogwash grease wastewater zone under the action of a surfactant is greatly increased.

The invention discloses a strain capable of degradation of benzo[a]pyrene, and belongs to the technical field of biodegradation treatment. The benzo[a]pyrene degradation strain is identified as Achromobacter sp., and the strain preservation number is CGMCC3684. The strain is prepared into the bacterium suspension with the bacterium content of 1.25*10<8> CFU/ml. With the strain of the present invention, the degradation removal rates of the materials with the initial benzo[a]pyrene concentrations of 0.11 mg/L, 0.34 mg/L, 0.71 mg/L, and 1.19 mg/L are respectively 74.35%, 84.66%, 78.91% and 68.33%.

The invention belongs to the technical field of biochemistry and particularly relates to achromobacter sp. JA81 with the collection number of CCTCC M 2011369 and a method for obtaining an electron-deficient alkane compound by converting (Z)-3-aryl-3-cyan-crylic acid, annular imide and unsaturated nitro compounds by using the achromobacter as a biocatalyst. In the method, the maximum enantiomeric excess reaches over 99 percent and the highest conversion ratio reaches 100 percent. The catalyst achromobacter is easy to prepare and mild in reaction condition, and is one of the effective methods for preparing a chiral electron-deficient alkane compound in an environment-friendly manner.

The invention discloses an achromobacter. The achromobacter is classified and named as Achromobacter sp, has a strain name of MBZ-2, is preserved in the China General Microbiological Culture Collection Center on August 13, 2020, and has a preservation number of CGMCC No. 20529. The achromobacter can effectively degrade p-toluenesulfonic acid and a downstream product herbicide quizalofop-p-ethyl.

The invention discloses an achromobacter sp. H380 for heavy metal cadmium pollution treatment. The achromobacter sp. H380 is preserved in the China center for type culture collection, and the preservation number is CCTCC NO: M2015291. According to the archromobacter, the heavy metal resistance spectrum is broad, the endurance capacity is strong, and the multi-antibiotic resistance is achieved, so that the environmental viability is strong, and the archromobacter is an excellent microbial material for heavy metal in-situ remediation. By means of the archromobacter, free cadmium ions in soil can be effectively removed, the acceptable daily intake of the cadmium ions for plants is obviously reduced, conversion from the free cadmium ions to combined cadmium ions is facilitated, therefore, the biological toxicity of the archromobacter is reduced, and the treatment of soil cadmium pollution is achieved.

The invention specifically discloses application of Achromobacter sp. MT-H in degradation of DEHP, which belongs to the technical field of biological treatment of environmental pollutants. The Achromobacter sp. MT-H is preserved in China Center for Type Culture Collection (CCTCC) on January 22, 2015, with an accession number of CCTCC M2015058. The Achromobacter sp. MT-H has high DEHP degradation efficiency and can perfectly degrade DEHP in wide pH and temperature ranges; thus, it is proved that MT-H can be used as an excellent DEHP degrading bacterium in biological treatment of DEHP pollution.

The invention relates to an Achromobacter sanius strain JD417 and application thereof, the strain is preserved in the China Center for Type Culture Collection on July 2, 2021, and the preservation number is CCTCC NO: M 2021813. The invention also relates to an application of the Achromobacter sanius strain JD417. The strain JD417 can tolerate sulfonamide compounds (antibiotics) with high concentration (50mg/L), and not only can degrade the sulfonamide compounds, but also can degrade nitrogen substances. The strain can maintain survival by taking nitrate nitrogen as a sole nitrogen source and sulfamethazine as a sole carbon source, effectively reduces total nitrogen, nitrate nitrogen and sulfonamide compounds in a water body, and has good ecological efficiency and application prospects in the aspect of treatment of environmental pollution such as sewage and the like.

The present invention relates to mutants of penicillin G acylase from Achromobacter sp. CCM 4824 and uses thereof, and more specifically relates to Mutants of penicillin G acylase in a wild type penicillin G acylase protein sequence including an Alpha subunit shown in SEQ ID NO.1 and a Beta subunit shown in SEQ ID NO.2 and a method of preparing (synthesizing) Beta-Lactam antibiotics by using theMutants of penicillin G acylase, wherein the Mutants of penicillin G acylase select at least one mutation from a population consisting of A65AlphaV, A102AlphaE, A106AlphaT, D74BetaQ, R120BetaC, T176BetaS, Y180BetaS, T193BetaI, Y248BetaF, F254BetaY, T292BetaS, F343BetaY, E366BetaV and T485BetaS as the feature. The Mutants of penicillin G acylase from Achromobacter sp. CCM 4824, having a specific mutation form, are characterized by multiple syntheses with high efficiency for multiple semi-synthesized Beta-Lactam antibiotics.

The present invention relates to a novel epoxide hydrolase enzyme which aims to achieve a high degree of resolution towards a broader range of substrates with high enantioselectivity and yields with minimal product inhibition. The invention further relates to a new bacterial strain Achromobacter sp. MTCC 5605 isolated from a petroleum-contaminated sludge sample, capable of producing the said enzyme. It is notable that the enzyme can be used as whole bacterial cell preparation, which allows continuous hydrolysis of substrates at even higher concentration and have an advantage of being recycled. The invention further relates to a process for the hydrolysis of different aryl epoxides which are potential synthons of intermediates for the synthesis of chiral amino alcohols and bioactive compounds like β-blockers.

The present invention relates to mutants of penicillin G acylase from Achromobacter sp. CCM 4824 and uses thereof, and more specifically relates to Mutants of penicillin G acylase in a wild type penicillin G acylase protein sequence including an Alpha subunit shown in SEQ ID NO.1 and a Beta subunit shown in SEQ ID NO.2 and a method of preparing (synthesizing) Beta-Lactam antibiotics by using the Mutants of penicillin G acylase, wherein the Mutants of penicillin G acylase select at least one mutation from a population consisting of A65AlphaV, A102AlphaE, A106AlphaT, D74BetaQ, R120BetaC, T176BetaS, Y180BetaS, T193BetaI, Y248BetaF, F254BetaY, T292BetaS, F343BetaY, E366BetaV and T485BetaS as the feature. The Mutants of penicillin G acylase from Achromobacter sp. CCM 4824, having a specific mutation form, are characterized by multiple syntheses with high efficiency for multiple semi-synthesized Beta-Lactam antibiotics.