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Method of treating oil contaminated soil and its special bacterin group

A technology of oil pollution and soil, applied in the restoration of contaminated soil, biochemical equipment and methods, bacteria, etc., to achieve the effect of easy large-scale cultivation, simple cultivation method, simple and applicable process

Inactive Publication Date: 2008-02-06
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, targeted screening of hydrocarbon-degrading bacteria suitable for lower temperature conditions (10°C to 20°C) for the treatment of oily soil in low-temperature areas or low-temperature seasons has not been reported yet.
[0009] Although there are many technical researches on bioremediation of oil-contaminated soil, these studies are either limited to the influence of a single factor of bacteria or the synergistic influence of bacteria, or only focus on the influence of a certain environmental factor such as nutrients, but not on the influence of bacteria. There is no report on the research on synergistic optimization affected by various factors such as microorganisms and nutrients

Method used

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  • Method of treating oil contaminated soil and its special bacterin group
  • Method of treating oil contaminated soil and its special bacterin group
  • Method of treating oil contaminated soil and its special bacterin group

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, the screening of degrading flora

[0040] The oil-contaminated soils of Chunlei Pumping Station Well Site and Nangang Pao Well Site of Daqing Oilfield were analyzed for analysis. The physical and chemical properties of the soil are shown in Table 1.

[0041] Within 15m around the wellhead of the abandoned well site near the Chunlei Pumping Station of Daqing Oilfield and the wellhead of the abandoned well site near Nangangpao, take the same amount of 5-20cm surface soil in the four directions of east, west, south and north, remove roots, leaves and stones and mix well , sealed in a sterile sample bag. The soil samples were stored at a low temperature of 0-4°C, and the microorganisms in the soil were detected and screened according to Table 2 (Du Lianxiang. Experimental Technology of Industrial Microbiology. Tianjin Science and Technology Press. 1992, 93-100).

[0042] Table 1. Analysis of physical and chemical properties of the tested soil

[0043] ...

Embodiment 2

[0055] Embodiment 2, biological treatment of oily soil in Daqing Oilfield

[0056] 1. Experimental facilities and materials

[0057] 1. Experimental materials:

[0058] Xanthomonas sp. dn1, Flavobacterium sp. dn2, Pseudomonas sp. dc2, Pseudomonas sp. dy1, and colorless Achromobacter sp. dy3 five strains of crude oil degrading bacteria.

[0059] Cultivate the above five strains in the culture medium at 35-38°C for 2 days to obtain the bacterial liquid, the number of bacterial cells in the bacterial liquid is 9.8×10 10 bacteria / mL bacterial liquid, 9.7×10 10 bacteria / mL bacterial liquid, 9.5×10 10 bacteria / mL bacterial liquid, 9.5×10 10 bacteria / mL bacterial liquid, 9.7×10 10 Bacteria / mL bacterial liquid, the medium used is: beef extract 3g / L, peptone 10g / L, NaCl 5g / L, water 1000mL, pH7.0~7.2.

[0060] Carrier: columnar activated carbon carrier.

[0061] Nutrients: The nutrients used are urea, (NH 4 ) 2 HPO 4 , and vitamin solution; the formula of vitamin solution is ...

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Abstract

The microbial pool provided by present invention is formed from Xanthomonas sp. dn 1 CGMCC No.1299, Flavobacterium sp.dn 2 CGMCC No. 1298, pseudomonas sp. dc 2 CGMCC No. 1297, Pseudomonas sp. dy1 CGMCC No. 1296 and Achromobacter sp. dy 3 CGMCC No. 1295. The above-mentioned microbial pool can be added into the soil polluted by oil, and can be used for degrading oil pollutant.

Description

technical field [0001] The invention relates to a method for treating soil pollution and its special flora, in particular to a method for treating low-temperature, weathered crude oil polluted soil and its special flora. Background technique [0002] Soil is an important part of the ecological environment and a repository of biogeochemical cycles of matter. However, in recent years, the phenomenon of soil pollution has become more and more serious, and the quality of the soil environment has been deteriorating. At the same time, the polluted soil directly endangers human health by forming secondary pollution to surface water and groundwater and entering the human body through the soil-plant system through the food chain. Therefore, the protection and management of the soil ecological environment has attracted widespread attention. The research and development of soil pollution control technology has become a hot spot in the current domestic and foreign environmental protect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20B09C1/10
Inventor 李广贺金文标张旭钟毅
Owner TSINGHUA UNIV
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