70 results about "Flavobacterium sp." patented technology

The invention relates to a method for biologically purifying industrial circulating water. The method is characterized in that a biodegradation reactor is arranged in a circulating water system; nitrobacteria and denitrifiers are taken as inocula in the biodegradation reactor; at least part of the circulating water enters the biodegradation reactor to be biologically treated; the water from the biodegradation reactor enters the circulating water system; and the denitrifiers contain one or two of kocuriapalustris FSDN-A, arthrobactercreatinolyticus FDN-1 and flavobacteriummizutaii FDN-2. Compared with the prior art, the method has the advantages that the biological treatment method is adopted to treat the nutrient sources needed by microorganisms before the circulating water enters the device, so that the microorganisms brought in by the circulating water in the circulating process are difficult to survive, and then growth and reproduction of the microorganisms in the circulating water system are controlled.

The invention discloses Flavobacterium sp.B29 CGMCC No. 3699 capable of producing blue pigment and a fermentation and extraction method thereof for preparing the crude preparation of the obtained blue pigment. The method comprises the following steps: inoculating proliferated strains in liquid PDA culture medium, culturing the strains at 25 DEG C and 200rpm for 12-24 hours, inoculating the strains in PDA culture medium by inoculation percent of 2-10 percent, and fermenting the strains for 48 hours; and centrifugally collecting thalli in fermented culture, using ethanol solution with organic reagent content of 50 percent as extracting agent, and concentrating and drying the obtained extract to obtain. The blue pigment has certain anti-oxidization function and good thermostability and photostability. The invention provides novel strains capable of producing the blue pigment and also provides a novel means for the industrialized production of natural blue pigment and the application scope is wide.

Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

The present invention is directed to a DNA sequence comprising one or more DNA sequences selected from the group consisting of a DNA sequence which encodes the GGPP synthase of Flavobacterium sp. R1534 (crtE), a DNA sequence which encodes the prephytoene synthase of Flavobacterium sp. R1534 (crtB), a DNA sequence which encodes the phytoene desaturase of Flavobacterium sp. R1534 (crtI), a DNA sequence which encodes the lycopene cyclase of Flavobacterium sp. R1534 (crtY) or a DNA sequence which encodes the beta -carotene hydroxylase of Flavobacterium sp. R1534 (crtZ) or DNA sequences which are substantially homolgous, vectors comprising such DNA sequences and / or a DNA sequence which encodes the beta -carotene beta 4-oxygenase of Alcaligenes strain PC-1 (crt W) or a DNA sequence which is substantially homologous, cells which are transformed by such DNA sequences and / or vectors, a process for the preparation of a desired carotenoid or a mixture of carotenoids by cultering such transformed cells and a process for the preparation of a food or feed composition. <IMAGE>

The invention relates to a straw rapid degradation microbial inoculum composition. The composition comprises 10%-20% of a degradation bacterium complex microbial inoculum, 40%-50% of peanut straw, 10%-15% of peanut shells, 20%-25% of fruit and vegetable residues and 20%-30% of earthworm cast. The degrading bacterium complex microbial inoculant is prepared from ZL-2 brevibacterium friariorum, lactobacillus acidophilus, Beijerinckia indica, paenibacillus mucilaginosus N6, Rhodopseudomonas and flavobacterium johnsonii; and the straw rapid degradation bacterial agent composition is combined with a film mulching composting technology for use, so that a fermentation heap body can be rapidly heated, the composting fermentation efficiency can be greatly improved, the composting decomposition degree is remarkably improved after fermentation is finished, the composting fermentation effect is improved, the content of organic matters in the produced fertilizer is higher (55.8%), the nutrients are more comprehensive, the absorption of plants is more facilitated, the yield of the rape can be increased by 57.5%, and the quality of the rape is effectively improved.

The invention discloses a bifunctional strain capable of degrading cellulose and starch as well as a separating and screening method and application of the bifunctional strain and relates to a bifunctional strain as well as a separating and screening method and application thereof, for solving the problem of low degradation speed of household garbage caused by the low temperature limitation. The strain is flavobacterium sp. FL-1, and is collected in China General Microbiological Culture Collection Center (CGMCC) on January 4, 2015 with a collection number of CGMCC No. 10272. The separating method comprises the following steps of firstly, preparing a doubling dilution liquid with different concentrations; secondly, enabling colonies to appear in the plate; thirdly, carrying out low-temperature constant-temperature incubation; and fourthly, separating and purifying until a pure strain is obtained. The bifunctional strain can simultaneously degrade cellulose and amylase and has the characteristic of high degradation efficiency of cellulose and starch. A culture medium used in screening is strong in pertinency. The method has the advantages of short process time, resource conservation, low cost and simple device.

The invention discloses a salt-tolerant microbial bacterial agent and a preparation method thereof. The microbial bacterial agent contains Staphylococcus coli FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrogeni DN-3 and Methylobacterium sdn-3. The microbial bacterial agent can realize the removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has good wastewater treatment effect, and can realize short-range nitrification and denitrification or simultaneous nitrification and denitrification denitrification while removing COD. Staphylococcus koshii FSDN-C can utilize a variety of carbon sources and has a certain salt tolerance, which can be used in the treatment of high-salt wastewater. At the same time, the strain can secrete a substance to enhance sludge flocculation under environmental stimulation sex. This further broadens the scope of application of the microbial agent.

The invention relates to a flavobacterium columnare transgenic engineering oral vaccine. Preparation steps are as follows: (1) analysis of a mature peptide fragment sequence of a protective antigenicgene, namely lip, of flavobacterium columnare; (2) construction of shuttle plasmids; (3) screening of recombinant strains; (4) culture of the recombinant strains; and (5) preparation of the oral vaccine. The vaccine is an oral vaccine and is inoculated in an oral immunization mode, the oral vaccine is convenient to use compared with injection immunization and immersion immunization vaccines, morelabor force is saved, and the flavobacterium columnare transgenic engineering oral vaccine has the advantage of being not limited by the size of fish bodies.

Provided is a gene sequence for endo-alginate lyase Alg2A derived from Flavobacterium sp. strain S20 freshly isolated from soil samples, and a preparation method and application therefor. Also provided is a method of preparing the alginate lyase Alg2A, i.e. a technical method utilizing genetic engineering, in which the Alg2A gene is cloned to an Escherichia coli expression vector to obtain an Escherichia coli recombinant strain that can heterologously express said lyase. Using said strain to heterologously express the prepared alginate lyase Alg2A has the function of decomposing sodium alginate and preparing sodium alginate oligosaccharide. The present invention applies to the chemical, agricultural, and food industries, as well as to livestock feed processing, to the field of medicine, and to the field of genetic engineering of marine algae.

The invention discloses a strain of Flavobacter johniii producing alginate lyase, which belongs to the field of biotechnology. The present invention isolates Flavobacterium johnsoniae WX-11 from soil and river water samples, that is, Flavobacterium johnsoniae CGMCC No.14444. The strain has simple nutritional requirements and short fermentation time, and is used in the production of alginate lyase The production cycle can be significantly shortened and the production cost can be reduced. The invention also discloses the use of the strain to prepare alginate lyase and reduce the viscosity of high-viscosity raw materials containing alginate. The alginate lyase produced by the Flavobacterium johniiii WX-11 provided by the present invention can degrade sodium alginate to generate biologically active fucoidan oligosaccharides, which can be widely used in the fields of agriculture, food, feed addition, medicine and seaweed processing, etc. .

The invention discloses Flavobacterium sp.B29 CGMCC No. 3699 capable of producing blue pigment and a fermentation and extraction method thereof for preparing the crude preparation of the obtained blue pigment. The method comprises the following steps: inoculating proliferated strains in liquid PDA culture medium, culturing the strains at 25 DEG C and 200rpm for 12-24 hours, inoculating the strains in PDA culture medium by inoculation percent of 2-10 percent, and fermenting the strains for 48 hours; and centrifugally collecting thalli in fermented culture, using ethanol solution with organic reagent content of 50 percent as extracting agent, and concentrating and drying the obtained extract to obtain. The blue pigment has certain anti-oxidization function and good thermostability and photostability. The invention provides novel strains capable of producing the blue pigment and also provides a novel means for the industrialized production of natural blue pigment and the application scope is wide.