71 results about "Flavobacterium sp." patented technology

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.

The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg / L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.

The invention relates to a bacterial strain used for short-cut denitrification for nitrogen removal and its application. The bacterial strain used for short-cut denitrification for nitrogen removal is flavobacterium mizutaii FDN-2, which is preserved in China general microbiological culture collection center on March 11, 2012 and has a preservation number of CGMCC NO.3659. The bacterial strain can directly utilize nitrite nitrogen as a substrate to complete a short-cut denitrification process. When treating ammonia-containing wastewater with the flavobacterium mizutaii FDN-2 of the invention,the process is simple, and the flavobacterium mizutaii FDN-2 can tolerate a high concentration organic carbon source. When the flavobacterium mizutaii FDN-2 in the invention is put into use, a systemcan be rapidly initiated, so that the flavobacterium mizutaii FDN-2 boasts very broad application prospects in various nitrogen removal processes of wastewater.

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

The invention discloses a vitamin K2 and preparation process thereof. According to the process, 1-hydroxyl-2-naphthoic acid resistant flavobacterium strain HNA12-D is fermented and cultured, and vitamin K2 efficiently metabolizes in the strain through the batch feeding of glycerin serving as the carbon source, peptone serving as the nitrogen source and precursor substances, and is extracted by using organic solvents and separated by macroporous resin, and the yield and purity of the product are improved. The total output of the vitamin K2 crude product is 200-600 mg / L after the flavobacterium strain HNA12-D of the invention is fermented and cultured for 120-144 hours, and the product purity reaches 80-99 percent after absorption chromatography; compared with the prior art, the yield output and the product purity are greatly improved, and the product can be used in industrial production.

The invention discloses a gene sequence of Incision alginate lyase Alg2B from Flavobacterium strain (Flavobacterium sp. S20). The invention also provides a method for preparing novel alginate lyase, which is characterized in that by using a gene engineering technical method, the gene of the alginate lyase is cloned to an escherichia coli expression vector, the escherichia coli recombinant strain capable of realizing heterogenous expression of enzyme can be obtained, the recombinant strain is used for heterogenous expression of the alginate lyase Alg2B, and thereby monosaccharide and oligosaccharides produced by sodium alginate can be degraded. The provided alginate lyase Alg2B can be widely used in the fields of agriculture, food, feed addictives, medicine and alga genetic engineering.

The invention discloses a novel flavobacterium strain, a cultivation method for the strain and application of the strain in the aspect of producing agarase. The provided strain which can be used for producing the agarase is flavobacterium sp. CGMCC NO.2342, and is specially characterized in that the strain can generate the agarase without agar, and 0.05 to 0.25 percent of agar powder or a certain carbon source can be added in a liquid culture medium. The provided method for producing the agarase cultivates the flavobacterium sp. in a fermentation culture medium; and the crude enzyme liquid of the obtained agarase can be continuously concentrated and purified, and also can be directly used for degrading the agar to produce agar oligosaccharide. The agarase produced by the strain has high activity, produces various agarase, and can be expected to form various series of agar oligosaccharide.

The invention relates to a method for biologically purifying industrial circulating water. The method is characterized in that a biodegradation reactor is arranged in a circulating water system; nitrobacteria and denitrifiers are taken as inocula in the biodegradation reactor; at least part of the circulating water enters the biodegradation reactor to be biologically treated; the water from the biodegradation reactor enters the circulating water system; and the denitrifiers contain one or two of kocuriapalustris FSDN-A, arthrobactercreatinolyticus FDN-1 and flavobacteriummizutaii FDN-2. Compared with the prior art, the method has the advantages that the biological treatment method is adopted to treat the nutrient sources needed by microorganisms before the circulating water enters the device, so that the microorganisms brought in by the circulating water in the circulating process are difficult to survive, and then growth and reproduction of the microorganisms in the circulating water system are controlled.

The invention discloses Flavobacterium sp.B29 CGMCC No. 3699 capable of producing blue pigment and a fermentation and extraction method thereof for preparing the crude preparation of the obtained blue pigment. The method comprises the following steps: inoculating proliferated strains in liquid PDA culture medium, culturing the strains at 25 DEG C and 200rpm for 12-24 hours, inoculating the strains in PDA culture medium by inoculation percent of 2-10 percent, and fermenting the strains for 48 hours; and centrifugally collecting thalli in fermented culture, using ethanol solution with organic reagent content of 50 percent as extracting agent, and concentrating and drying the obtained extract to obtain. The blue pigment has certain anti-oxidization function and good thermostability and photostability. The invention provides novel strains capable of producing the blue pigment and also provides a novel means for the industrialized production of natural blue pigment and the application scope is wide.

Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

The present invention is directed to a DNA sequence comprising one or more DNA sequences selected from the group consisting of a DNA sequence which encodes the GGPP synthase of Flavobacterium sp. R1534 (crtE), a DNA sequence which encodes the prephytoene synthase of Flavobacterium sp. R1534 (crtB), a DNA sequence which encodes the phytoene desaturase of Flavobacterium sp. R1534 (crtI), a DNA sequence which encodes the lycopene cyclase of Flavobacterium sp. R1534 (crtY) or a DNA sequence which encodes the beta -carotene hydroxylase of Flavobacterium sp. R1534 (crtZ) or DNA sequences which are substantially homolgous, vectors comprising such DNA sequences and / or a DNA sequence which encodes the beta -carotene beta 4-oxygenase of Alcaligenes strain PC-1 (crt W) or a DNA sequence which is substantially homologous, cells which are transformed by such DNA sequences and / or vectors, a process for the preparation of a desired carotenoid or a mixture of carotenoids by cultering such transformed cells and a process for the preparation of a food or feed composition. <IMAGE>

The invention relates to application of alpha-L-rhamnosidase derived from bacteria in efficient production of hesperetin-7-O-glucoside. The nucleotide sequence of the alpha-L-rhamnosidase FjRha is shown as SEQ ID NO.1, and the gene is obtained by cloning from a genome of flavobacterium johnsonii. The alpha-L-rhamnosidase FjRha can specifically hydrolyze an artificial substrate and a natural substrate containing alpha -1, 6 rhamnoside bonds, and can efficiently and specifically catalyze hesperidin to prepare hesperetin-7-O-glucoside, and the conversion rate of catalyzing 3g / L hesperidin to generate the hesperetin-7-O-glucoside under the conditions that the enzyme dosage is 8U / mL, the pH value is 7.0 and the temperature is 37 DEG C is up to 95% or above. The method has the advantages of simple steps, low cost, mild conditions, environmental friendliness and the like, and has a wide application prospect.

The invention relates to the field of genetic engineering, in particular to low-temperature alpha-galactosidase GalA17, a gene thereof and application thereof, and provides an alpha-galactosidase GalA17 derived from flavobacterium sp. TN17 in gastrointestinal tracts of long-horned beetles, and the amino acid sequence of the alpha-galactosidase GalA17 is shown as SEQ ID No.1. The invention provides an encoding gene galA17 for encoding the alpha-galactosidase. The alpha-galactosidase has the following properties that: the optimum pH is 5.5, the optimum temperature is at 45 DEG C, and enzyme activity is over 55 percent at the temperature of between 30 and 50 DEG C; the pH stability is excellent; the protease resistibility and capability of hydrolyzing various substrates are excellent; and the alpha-galactosidase can be used as feed or food additives applied in feed and food industry.

The invention relates to an organophosphorus hydrolase genome expressed in escherichia coli and application thereof. The organophosphorus hydrolase genome comprises an OpdS gene derived from flavobacterium and obtained after optimization according to codon preference of escherichia coli, and PnpAS, PnpBS, PnpCS, PnpDS and PnpES genes derived from pseudomonas and obtained after optimization according to codon preference of escherichia coli; the nucleotide sequences are shown as SEQ ID NO.1-6; the PnpAS, PnpBS, PnpCS, PnpDS and PnpES genes obtained after optimization are fused with a T7 promoterand a terminator respectively; a corresponding gene expression cassette is constructed, then connected into an escherichia coli expression vector, transformed into escherichia coli and successfully expressed in the escherichia coli; and an obtained positive strain has a good degradation effect on organophosphorus pesticides including parathion-methyl and paraoxon-methyl.

The invention relates to a straw rapid degradation microbial inoculum composition. The composition comprises 10%-20% of a degradation bacterium complex microbial inoculum, 40%-50% of peanut straw, 10%-15% of peanut shells, 20%-25% of fruit and vegetable residues and 20%-30% of earthworm cast. The degrading bacterium complex microbial inoculant is prepared from ZL-2 brevibacterium friariorum, lactobacillus acidophilus, Beijerinckia indica, paenibacillus mucilaginosus N6, Rhodopseudomonas and flavobacterium johnsonii; and the straw rapid degradation bacterial agent composition is combined with a film mulching composting technology for use, so that a fermentation heap body can be rapidly heated, the composting fermentation efficiency can be greatly improved, the composting decomposition degree is remarkably improved after fermentation is finished, the composting fermentation effect is improved, the content of organic matters in the produced fertilizer is higher (55.8%), the nutrients are more comprehensive, the absorption of plants is more facilitated, the yield of the rape can be increased by 57.5%, and the quality of the rape is effectively improved.

The invention discloses a bifunctional strain capable of degrading cellulose and starch as well as a separating and screening method and application of the bifunctional strain and relates to a bifunctional strain as well as a separating and screening method and application thereof, for solving the problem of low degradation speed of household garbage caused by the low temperature limitation. The strain is flavobacterium sp. FL-1, and is collected in China General Microbiological Culture Collection Center (CGMCC) on January 4, 2015 with a collection number of CGMCC No. 10272. The separating method comprises the following steps of firstly, preparing a doubling dilution liquid with different concentrations; secondly, enabling colonies to appear in the plate; thirdly, carrying out low-temperature constant-temperature incubation; and fourthly, separating and purifying until a pure strain is obtained. The bifunctional strain can simultaneously degrade cellulose and amylase and has the characteristic of high degradation efficiency of cellulose and starch. A culture medium used in screening is strong in pertinency. The method has the advantages of short process time, resource conservation, low cost and simple device.

The invention discloses a salt-tolerant microbial bacterial agent and a preparation method thereof. The microbial bacterial agent contains Staphylococcus coli FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrogeni DN-3 and Methylobacterium sdn-3. The microbial bacterial agent can realize the removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has good wastewater treatment effect, and can realize short-range nitrification and denitrification or simultaneous nitrification and denitrification denitrification while removing COD. Staphylococcus koshii FSDN-C can utilize a variety of carbon sources and has a certain salt tolerance, which can be used in the treatment of high-salt wastewater. At the same time, the strain can secrete a substance to enhance sludge flocculation under environmental stimulation sex. This further broadens the scope of application of the microbial agent.

The invention relates to a flavobacterium columnare transgenic engineering oral vaccine. Preparation steps are as follows: (1) analysis of a mature peptide fragment sequence of a protective antigenicgene, namely lip, of flavobacterium columnare; (2) construction of shuttle plasmids; (3) screening of recombinant strains; (4) culture of the recombinant strains; and (5) preparation of the oral vaccine. The vaccine is an oral vaccine and is inoculated in an oral immunization mode, the oral vaccine is convenient to use compared with injection immunization and immersion immunization vaccines, morelabor force is saved, and the flavobacterium columnare transgenic engineering oral vaccine has the advantage of being not limited by the size of fish bodies.

The invention discloses pueraria lobata feed as well as a preparation method and an application thereof. The pueraria lobata feed is prepared from 55%-70% by weight of pueraria lobata, 20%-30% by weight of fermentation additives and 10%-15% by weight of mixed microorganisms through anaerobic fermentation, wherein the fermentation additives comprise spirulina extract, ginkgo biloba extract, fructuslycii extract, bran, 5%-10% calcium chloride, manganese sulfate and ferrous chloride; the mixed microorganisms comprise cellulose decomposition bacteria, flavobacterium, bacillus subtilis and lactobacillus plantarum. The pueraria lobata feed has the advantages as follows: the obtained pueraria lobata feed not only has acid fragrance, but also has low requirement for storage, feed palatability isimproved, appetite of animals is stimulated, and feed intake is increased.

The invention provides a microbial agent including ensifer adhaerens, acinetobacter calcoaceticus and flavobacterium chungangense. The invention also provides a compound fertilizer including the microbial agent, humic acid and an inorganic fertilizer, wherein the number of the microbial agent is 1-500000000 / g, and the mass ratio of humic acid to the inorganic fertilizer is 7:3. The inorganic fertilizer contains three elements of nitrogen, phosphorus and potassium, and the ratio of N, P2O5 and K2O is 8:30:4. The microbial compound fertilizer is rich in nutrition and comprehensive in functions, and can significantly improve the yield and quality of cucumber. Compared with a common chemical fertilizer, under a condition of the same amount of input, the microbial compound fertilizer can allows the yield to be increased by 10-60% and the soluble sugar to be increased by 10-20%.

Provided is a gene sequence for endo-alginate lyase Alg2A derived from Flavobacterium sp. strain S20 freshly isolated from soil samples, and a preparation method and application therefor. Also provided is a method of preparing the alginate lyase Alg2A, i.e. a technical method utilizing genetic engineering, in which the Alg2A gene is cloned to an Escherichia coli expression vector to obtain an Escherichia coli recombinant strain that can heterologously express said lyase. Using said strain to heterologously express the prepared alginate lyase Alg2A has the function of decomposing sodium alginate and preparing sodium alginate oligosaccharide. The present invention applies to the chemical, agricultural, and food industries, as well as to livestock feed processing, to the field of medicine, and to the field of genetic engineering of marine algae.

The invention discloses a strain of Flavobacter johniii producing alginate lyase, which belongs to the field of biotechnology. The present invention isolates Flavobacterium johnsoniae WX-11 from soil and river water samples, that is, Flavobacterium johnsoniae CGMCC No.14444. The strain has simple nutritional requirements and short fermentation time, and is used in the production of alginate lyase The production cycle can be significantly shortened and the production cost can be reduced. The invention also discloses the use of the strain to prepare alginate lyase and reduce the viscosity of high-viscosity raw materials containing alginate. The alginate lyase produced by the Flavobacterium johniiii WX-11 provided by the present invention can degrade sodium alginate to generate biologically active fucoidan oligosaccharides, which can be widely used in the fields of agriculture, food, feed addition, medicine and seaweed processing, etc. .

The invention discloses a preparation method of flavobacterium columnare genetic recombination vaccines. The preparation method of flavobacterium columnare genetic recombination vaccines comprises the steps of (1) constructing expression carriers; (2) expressing and purifying target protein; (3) adjusting the concentration of the purified protein so as to obtain the flavobacterium columnare genetic recombination subunit vaccines which can be used directly. The vaccines prepared by the invention have the advantages of high safety, stable immune effect, high production efficiency and the like.

The invention discloses Flavobacterium sp.B29 CGMCC No. 3699 capable of producing blue pigment and a fermentation and extraction method thereof for preparing the crude preparation of the obtained blue pigment. The method comprises the following steps: inoculating proliferated strains in liquid PDA culture medium, culturing the strains at 25 DEG C and 200rpm for 12-24 hours, inoculating the strains in PDA culture medium by inoculation percent of 2-10 percent, and fermenting the strains for 48 hours; and centrifugally collecting thalli in fermented culture, using ethanol solution with organic reagent content of 50 percent as extracting agent, and concentrating and drying the obtained extract to obtain. The blue pigment has certain anti-oxidization function and good thermostability and photostability. The invention provides novel strains capable of producing the blue pigment and also provides a novel means for the industrialized production of natural blue pigment and the application scope is wide.

The invention discloses a biodegradation method for aflatoxin in gunited corn bran, and relates to the technical field of feed processing. The biodegradation method for the aflatoxin in the gunited corn bran is characterized in that the aflatoxin in the gunited corn bran is degraded by flavobacterium aurantiacum and culture medium extracellular crude extract liquid and is subjected to ultraviolettreatment, wherein the flavobacterium aurantiacum is lactoperoxidase, and the ratio of flavobacterium aurantiacum crude protein extract in an aqueous solution is 10-40%; and the culture medium extracellular crude extract liquid is stenotrophomonas maltophilia culture medium extracellular crude extract liquid. According to the biodegradation method for the aflatoxin in the gunited corn bran, afterthe flavobacterium citrinum protein extract is mixed with the aqueous solution, the aflatoxin B1 in the corn bran is degraded, and the ratio of the aflatoxin subjected to primary degradation in the corn bran is reduced to 76%; and biological enzyme degradation is performed on the aflatoxin by using the extracellular crude extract liquid.

The invention belongs to the field of molecular biology, and discloses an application of a flavobacterium columnar virulence protein in pathogen detection and low virulent strain preparation, and the amino acid sequence of the flavobacterium columnar virulence protein is shown as SEQ ID NO.1 or SEQ ID NO.3. The invention further discloses a preparation method of the flavobacterium columnar virulence protein. An antibody is designed by utilizing the virulence protein and can be used for detecting pathogenic bacteria of flavobacterium columnar. A virulence gene for coding the protein is deleted from flavobacterium cylindricum, so that a flavobacterium cylindricum attenuated strain can be obtained, and the capability of the flavobacterium cylindricum for infecting a fish cell line and a fish body can be reduced by utilizing the attenuated strain; further, the strain is used as a candidate vaccine strain to realize immune prevention and control of columnar diseases caused by flavobacterium cylindricum.

The invention discloses a fish gill-rot disease pathogenic flavobacterium johnsoniae attenuated live vaccine and a construction method thereof. Wild-type flavobacterium johnsoniae is subjected to continuous passage induction mutation to make the toxicity weakened by streptomycin, and a flavobacterium johnsoniae attenuated mutant strain M170 is obtained; the live vaccine prepared by using the attenuated strain immunizes grass carp, mandarin fish and the like through injection and soaking, the immune protective rates all can reach 73.1% or more, and the immune protection period reaches 8 months or more; the live vaccine has the advantages of small immunizing dose, high titer, high protection rate, long protection period and the like, is used for immunization by the soaking way, can prevent occurrence of bacterial gill-rot disease of grass carp, mandarin fish and the like, and has good application value and broad market prospect.

The invention discloses alginate lyase Alg2A and a preparation method and application thereof. According to the preference of pichia pastoris codon, an alginate lyase coding gene in Flavobacterium sp.S20 is optimized, and the optimized nucleic acid sequence is shown in SEQ ID NO. 2. Furthermore, the optimized alginate lyase coding gene is efficiently secreted and expressed by means of a pichia pastoris expression system, the alginate lyase is obtained, and the amino acid sequence of the alginate lyase is shown in SEQ ID NO.1. The obtained alginate lyase has high hydrolytic activity to a sodium alginate substrate, and crude enzyme liquid produced by shaking flask for fermentation has the capacity of completely degrading 1 g of sodium alginate by 0.1 ml (protein content is about 0.042 mg),and the alginate lyase Alg2A has the good industrial application prospect.

The invention provides a preparation method and application of flavobacterium and flavobacterium secreta and relates to the technical field of microorganisms. The flavobacterium has a preservation number of M2017262. The flavobacterium secreta have alga-lysing activity, can inhibit and kill algae in red tide and can well treat red tide.