Blue pigment producing bacteria and method for preparing crude preparation by using the same

A blue pigment and preparation technology, applied in the field of bioengineering, can solve the problem that the type and quantity cannot meet the needs of the research and development of natural blue pigment

Inactive Publication Date: 2012-04-18
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The types and quantities of these strains are far from meeting the needs of various industries for the research and development of natural blue pigments

Method used

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  • Blue pigment producing bacteria and method for preparing crude preparation by using the same
  • Blue pigment producing bacteria and method for preparing crude preparation by using the same
  • Blue pigment producing bacteria and method for preparing crude preparation by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the cultivation of Flavobacterium sp.B29 CGMCC No.3699

[0022] The preserved Flavobacterium sp.B29 (Flavobacterium sp.B29) CGMCC No.3699 is subcultured and activated on the PDA medium, and whether there is contamination by bacteria while detecting the color change of the colony; the activated bacterial strain is inoculated in the liquid TGY medium ( No agar), the liquid culture medium filling volume is 100mL / bottle (500mL Erlenmeyer flask), 25 ℃ of constant temperature, culture 72 hours under 200rpm, observe thalline growth and color change.

[0023] Flavobacterium sp.B29 CGMCC No.3699 culture experiments showed that white, moist colonies were first formed on the surface of TGY medium, and then gradually formed wrinkled, dry blue to blue-purple colonies; in liquid TGY medium, cultured Obvious white cells appeared in 24 hours, and the cells appeared blue after 72 hours.

Embodiment 2

[0024] Example 2: Preparation and light-absorbing properties of blue pigment crude preparation

[0025] Flavobacterium sp.B29 (Flavobacterium sp.B29) CGMCC No.3699 fermentation broth obtained as in Example 1, with 8000rpm, 3min centrifugation process to obtain the thalline, add 50mL 50% ethanol solution with the thalline obtained by per 100mL fermentation culture , 100rpm vibration extraction treatment for 1 hour, and then 8000rpm, 5min centrifugation, the obtained supernatant is the ethanol extract of blue pigment; the ethanol extract is placed at a constant temperature of 60°C for vacuum concentration and straight drying, and the obtained is blue pigment Pigment crude extract. The obtained blue pigment crude extract was dissolved in 50% ethanol solution, and a scanning analysis of 200-800nm ​​was carried out using a UV spectrophotometer. For the analysis results, see the attached figure 1 shown.

[0026] When analyzing the result table, the blue pigment produced by Flavoba...

Embodiment 3

[0027] Embodiment 3: Quantitative detection of blue pigment crude preparation

[0028] According to the method described in Example 2, the blue pigment crude preparation of Flavobacterium sp.B29 (Flavobacterium sp.B29) CGMCCNo.3699 was obtained and carried out vacuum freeze-drying to remove moisture; Accurately weigh the preparation of 1.000g dry treatment, use 50% The ethanol solution was fully dissolved, and the volume was adjusted to 100mL to prepare a blue pigment mother solution of 250mg / L; further dilution treatment was prepared to prepare a series of blue pigment solutions of 50, 100, 150, 200, and 250mg / L, and the blue pigment solution was prepared at a wavelength of 579nm Measure the absorbance value; draw the concentration-absorbance value standard curve with the blue pigment concentration as the horizontal axis and the absorbance value at 579nm wavelength as the vertical axis.

[0029] For the measurement results of the corresponding relationship between the concent...

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Abstract

The invention discloses Flavobacterium sp.B29 CGMCC No. 3699 capable of producing blue pigment and a fermentation and extraction method thereof for preparing the crude preparation of the obtained blue pigment. The method comprises the following steps: inoculating proliferated strains in liquid PDA culture medium, culturing the strains at 25 DEG C and 200rpm for 12-24 hours, inoculating the strains in PDA culture medium by inoculation percent of 2-10 percent, and fermenting the strains for 48 hours; and centrifugally collecting thalli in fermented culture, using ethanol solution with organic reagent content of 50 percent as extracting agent, and concentrating and drying the obtained extract to obtain. The blue pigment has certain anti-oxidization function and good thermostability and photostability. The invention provides novel strains capable of producing the blue pigment and also provides a novel means for the industrialized production of natural blue pigment and the application scope is wide.

Description

technical field [0001] The invention relates to the technical field of bioengineering, specifically, the invention relates to the technical field of bacterial strains for producing natural cyanine, crude preparations of natural cyanine and methods thereof. Background technique [0002] Pigment is a coloring agent, because it can significantly improve people's sensory stimulation to the properties of objects, it has become an important part of the printing and dyeing industry, food additives and cosmetics industries, and it is also one of the key factors that determine product quality. Commonly used pigments can be roughly divided into two categories: synthetic pigments and natural pigments. Synthetic pigments are organic pigments prepared by chemical synthesis methods, mainly made of aniline dyes separated from coal tar. They have the advantages of bright color, strong tinting power, stable properties and low price, and are widely used. . However, studies have shown that g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C09B61/00C12R1/20
Inventor 唐琦勇宋素琴朱静王浩
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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