A pyrene-degrading bacterium and its application

A technology of pyrene-degrading bacteria and strains, applied in the field of biodegradation treatment of contaminated soil and water

Inactive Publication Date: 2011-12-07
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]However, due to the lack of efficient PAH-degrading bacteria, the further development and application of PAH-contaminated soil bioremediation technology is restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Adding petroleum-contaminated soil to the open glass bottle with pyrene and basal medium for enrichment and domestication of pyrene-degrading microorganisms. Insert the enriched acclimatized soil into the shaped bottle. After culturing in an oscillator at a temperature of 25-30°C and a rotation speed of 100r / min for 5-7 days, the cycle transfer is carried out, and after the number of cycles is more than 8 times, a degrading bacterial group that can efficiently remove pyrene can be obtained. Make the bottom plate of agar solid medium with pyrene as the only carbon source and energy source in the ultra-clean workbench, make the top plate of agar solid medium containing pyrene-degrading bacteria on the bottom plate, and place the prepared medium plate in the incubator Cultured in medium for 10-15 days. In the ultra-clean workbench, insert the colony on the medium plate into the conical flask with pyrene, basal medium, trace metal liquid, vitamin C solution and adsorbent, ...

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PUM

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Abstract

The invention discloses a pyrene-degrading bacterium and its application, which belong to the technical field of biodegradation treatment. The pyrene-degrading bacterium was identified as Achromobacter sp., and the strain preservation number was CGMCC3637. The Genbank accession number of the 16S rDNA of this strain is EU220009.1. The bacterial strain is made into a bacterial suspension with a bacterial content of 1.25×108CFU / ml, and the initial concentration is respectively 0.11mg / l, 0.26mg / l, 0.52mg / l, and 1.14mg / l. The degradation of pyrene after 7 days The removal rates were 92.65%, 91.46%, 88.29%, and 89.67%, respectively.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, in particular to a pyrene-degrading bacterium and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of condensed ring compounds containing two or more benzene rings arranged in linear, angular or clustered shapes, with hydrophobicity, low solubility, low vapor pressure, High melting point and boiling point, high octanol-water partition coefficient and other characteristics. Polycyclic aromatic hydrocarbons have strong carcinogenic, teratogenic and mutagenic effects. Generally speaking, low-molecular-weight PAHs with 2-3 rings have significant acute toxicity, while some high-molecular-weight PAHs have potential carcinogenicity. Due to the presence of conjugated large π-bond electron systems in PAHs molecules, they have high stability, their photolysis and biodegradation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C02F3/02A62D3/02C12R1/025C02F101/32A62D101/20
CPCY02W10/10
Inventor 豆俊峰杜勇超丁爱中程莉蓉范福强陈海英
Owner BEIJING NORMAL UNIVERSITY
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