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Preparation method and application of grease degrading bacterium community

A technology for degrading bacteria and oil, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and oil/fat production. It can solve the problems of low efficiency of decomposing surfactants and the impact of microbial toxicity, and achieve low cost and high degradation efficiency. Improved effect

Inactive Publication Date: 2016-08-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of microorganisms to decompose surfactants by themselves is low, so it is a feasible method to promote its degradation by adding surfactants, and there are many types of surfactants
[0005] Although surfactants can increase the solubility of hydrophobic organic compounds in water systems, most surfactants have toxic effects on microorganisms

Method used

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  • Preparation method and application of grease degrading bacterium community
  • Preparation method and application of grease degrading bacterium community
  • Preparation method and application of grease degrading bacterium community

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] The preparation method of the grease-degrading flora of the present invention comprises the following specific steps:

[0035] (1) 65mg K 2 HPO 4 , 25.5mg KH 2 PO 4 , 133.8mg Na 2 HPO 4 12H 2 O, 500mg NH 4 Cl, 82.5mg CaCl 2, 67.5mg MgSO 4 ·7H 2 O, 0.75mg FeCl 3 ·6H 2 O was mixed with 1000mL deionized water; after adjusting the pH value to 7.0, sterilized at 121°C for 20min to obtain a liquid inorganic salt medium;

[0036] (2) Get 5g of the sediment sample collected from the kitchen swill waste grease wastewater area, add it to the aforementioned liquid inorganic salt culture medium, and then add the grease in the kitchen swill waste grease wastewater area accounting for 0.5% of the total volume; Then the mixture was placed in a constant temperature shaking box, and cultivated for 5 days at 30°C under stirring conditions with a rotation speed of 150r / min;

[0037] (3) Obtain a new liquid inorganic salt medium by the method described in step (1), get 10ml of...

Embodiment 1

[0044] In this example, oil-degrading bacteria and surfactant Tw-80 (c 1 Concentration, c 2 Concentration, c 3 Concentration) work together to degrade the grease collected from the kitchen swill waste grease wastewater area, the specific steps are as follows:

[0045] Take 50mL of the liquid inorganic salt culture medium, take 2mL of the oil-degrading bacteria, and put them into a 100mL conical flask; Grease in the area; then add surfactant Tw-80 (c 1 Concentration, c 2 Concentration, c 3 Concentration) 1mL; placed in a constant temperature shaking box, maintained at a temperature of 30°C and a rotation speed of 150r / min for cultivation to degrade the grease content of the added kitchen slops in the grease wastewater area.

[0046] Set up the triangular flask according to the above steps, and divide it into three groups; the three groups of treatments correspond to adding different surfactants with different concentrations set in the following table, except that the added...

Embodiment 2

[0053] In this example, grease-degrading bacteria were used to work together with surfactants Tw-80, SDS, and saponin to degrade grease collected from the kitchen swill waste grease wastewater area. The specific steps are as follows:

[0054] Take 50mL of the liquid inorganic salt culture medium, take 2mL of the oil-degrading bacteria, and put them into a 100mL conical flask; Then add 1mL of surfactant; place in a constant temperature shaking box, keep the temperature at 30°C, and cultivate at a speed of 150r / min to degrade the oil content of the added kitchen slops in the oily wastewater area.

[0055] Set up the triangular flask according to the above steps, and divide it into three groups; the three groups of treatments correspondingly add different surfactants with different concentrations set in the following table, except that the added surfactants are different, and the rest of the conditions are the same:

[0056] Table 2 Tw-80, SDS, saponin experimental group design ...

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Abstract

The invention relates to the technical field of biodegradation treatment, and aims at providing a preparation method and an application of a grease degrading bacterium community. The preparation method of the grease degrading bacterium community comprises the following steps: adding a deposit sample and grease collected from a food waste hogwash grease wastewater zone to an inorganic salt medium, and culturing the deposit sample and grease at 30DEG C under a rotating speed of 150r / min for 5d; repeatedly culturing the obtained material through the same technology 2 times to obtain a final liquid which is a bacterial liquid containing the grease degrading bacterium community; and carrying out DNA sequencing confirmation, wherein the grease degrading bacterium community comprises Stenotrophomonas maltophilia, Chryseobacterium spp, Achromobacter sp and enterobacteria. Onsite sampling (for achieving low cost) is realized, the grease degrading bacterium community is enriched and screened through a simple and convenient process, and grease wastewater in the food waste hogwash grease wastewater zone is effectively degraded; and the degradation efficiency in the treatment of the grease wastewater in the food waste hogwash grease wastewater zone under the action of a surfactant is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment, and in particular relates to a method for screening oil-degrading bacteria and its application to the treatment of oily waste water in kitchen slop waste oily waste water area under the joint action of the oil-degrading bacterial group and a surfactant. Background technique [0002] With the rapid development of my country's economy and the continuous rise of tourism. In recent years, the number of farmhouses has been increasing rapidly, and more and more slops (leftovers) and washing wastewater are being discharged. Its ingredients are complex and high in organic content, starting with the first: oil. If this kind of organic matter is discharged into rivers and lakes without treatment, it will pollute the environment. Grease floats on the water surface to form an oil film, which hinders oxygen transmission and threatens the survival of aquatic animals and plants. [0003] At p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C11B1/00C12R1/01C12R1/025
CPCC12N1/20C11B1/00
Inventor 赵和平钟亮温丽莲张吟陈嘉娴
Owner ZHEJIANG UNIV
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