Ethylbenzene degrading bacterium and screening method and application thereof

A screening method and technology for degrading bacteria, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of insufficient removal of low-concentration benzene pollutants, high cost, secondary pollution, etc.

Pending Publication Date: 2021-11-30
上海圣珑环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an ethylbenzene-degrading bacterium and its screening method and application, to overcome the damage to the soil and water environment caused by the above-mentioned technology, the removal of low-concentration benzene pollutants is not thorough enough, requires high cost, and is easy to cause Secondary pollution and other issues

Method used

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  • Ethylbenzene degrading bacterium and screening method and application thereof
  • Ethylbenzene degrading bacterium and screening method and application thereof
  • Ethylbenzene degrading bacterium and screening method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Strain acquisition

[0053] Contaminated soil was collected from a pesticide factory in Bengbu, Anhui, packed in brown bottles and transported back to the laboratory. The soil was added to the culture medium to prepare a mixture. After screening and domestication, a strain of highly efficient ethylbenzene-degrading bacteria was obtained, and the strain was tested for benzene series. Degradation verification, found that the strain can efficiently degrade benzene, toluene and ethylbenzene, p-xylene also has a certain degradation effect, and the strain has stable passage characteristics.

[0054] Gram staining of the strain was negative, and the shape of the strain was non-capsulated and achromobacter. Colonies on Luria-Bertani medium plates were regular, yellow, slightly raised, opaque and smooth.

[0055] 16S rDNA sequencing was carried out on it, and the measured 16S rDNA sequences were compared by BLAST. The comparison results showed that the nucleotide sequence of th...

Embodiment 2

[0070] Study on Degradation Characteristics of Strains (Different Ethylbenzene Concentration, Temperature, pH, Yeast Powder Concentration)

[0071] ED-2 was expanded in Luria-Bertani medium, centrifuged at 8000r / min for 5min, and resuspended in 1% inorganic salt medium to wash away carbon sources such as yeast powder. After repeating twice, the total volume remained unchanged. Measure the absorbance value, and add it into a 150mL serum bottle, which contains 20mL of inorganic salt medium containing ethylbenzene, and degrade the initial OD in the system 600 Value 0.1. In order to prevent ethylbenzene volatilization, cover and seal, shake culture at 28°C, 180rpm, and avoid light. Change the ethylbenzene concentration, pH value, yeast powder concentration, and temperature respectively. After 48 hours, take a sample to measure the residual concentration of ethylbenzene.

[0072] Degradation characteristics results such as Figure 4 As shown, ED-2 can tolerate 300mg / L of ethylben...

Embodiment 3

[0074] Study on Degradation of BTEX in Wastewater by Bacteria

[0075] 20 mL of inorganic salt medium containing 50 mg / L BTEX (benzene, toluene, ethylbenzene, o-xylene, m / p-xylene) was placed in a 150 mL serum bottle, and the inoculation step was the same as in Example 1. In order to prevent the volatilization of benzene series, cover and seal, shake culture at 28°C, 180rpm, and avoid light, take samples regularly, and measure the residual concentration of BTEX.

[0076] Figure 8 It shows that ED-2 completely degrades 50mg / L ethylbenzene and toluene within 92 hours, 76% of benzene is degraded, 57% of m- and p-xylene is degraded, and 47% of o-xylene is degraded.

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Abstract

The invention relates to an ethylbenzene degrading bacterium and a screening method and application thereof. The ethylbenzene degrading bacterium is Achromobacter, is named as Achromobacter sp.ED-2, and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO. M2021058, and the preservation time is January 14, 2021. The ethylbenzene degrading bacterium can completely degrade 100mg / L ethylbenzene within 48h, and can also degrade benzene, methylbenzene, m- and p-xylene and o-xylene. Through liquid chromatography detection, the bacterium has no intermediate product metabolic accumulation when degrading benzene, the degrading effect of the bacterium is stable, and support is provided for the biological purification practice process of benzene series polluted sites.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment and degradation, and relates to an ethylbenzene-degrading bacterium and a screening method and application thereof. Background technique [0002] Ethylbenzene is a highly toxic aromatic compound that is a constituent of petroleum hydrocarbons. Ethylbenzene usually enters the environment in the form of petroleum refining, plastics, resins, pesticide production, and pharmaceutical industry wastewater or waste gas discharge. Shi Zhen et al. reported that the detection frequency of ethylbenzene in lakes, rivers and other water quality monitoring is as high as 71.4%. The US Environmental Protection Agency (US EPA 1996) found that short-term exposure to ethylbenzene (above 0.7 ppm) can cause drowsiness, fatigue, headache, and mild eye and respiratory irritation. Chronic exposure to ethylbenzene can potentially damage the liver, kidneys, central nervous system and eyes. Therefore, ethylbenz...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C02F101/32C12R1/01
CPCC12N1/20C12N1/02C02F3/34C02F2101/322Y02A50/20
Inventor 罗启仕崔长征张磊常国兴常玲赵建锋
Owner 上海圣珑环境科技有限公司
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