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One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method

An alkaline medium, high-concentration technology, applied in the biological treatment of chromium slag leachate and chromium slag, and the field of alkaline chromium-containing wastewater, can solve problems such as chromium pollution, achieve simple composition, strong adaptability, and easy large-scale cultivation. Effect

Inactive Publication Date: 2007-12-12
CENT SOUTH UNIV
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Problems solved by technology

[0005] From the perspective of bacterial metallurgy, the present invention obtains a strain of bacteria capable of efficiently reducing high-concentration Cr(VI) in alkaline media through collection, separation, and domestication, and aims at the characteristics of the strain, and provides a simple and easy-to-large-scale cultivation. The easy and low-cost cultivation method provides bacteria that can be cultivated on a large scale for the efficient treatment of alkaline chromium-containing wastewater, chromium residue leachate and chromium residue by biological methods, and promotes the development of biological treatment of alkaline Cr(VI) technology to solve Chromium Pollution Plaguing the Development of my country's Chromium Salt and Chemical Industries

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  • One strain of bacterium of reducing high density hexabasic chromium in alkaline medium and its cultivating method

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Embodiment Construction

[0019] 1. Retrieve the bacterial samples from the vicinity of the chromium slag landfill, carry out enrichment culture for them, separate the effective strains on plates, pick the obligate strains for pure culture, and increase the concentration of hexavalent chromium step by step. Acclimate to obtain bacteria that can efficiently and quickly reduce 2000mg / LCr(VI).

[0020] The bacteria in the logarithmic phase were inoculated in 920mg / LCr(VI) 10L culture solution, wherein yeast extract was 1.5g / L, sodium lactate was 1.5g / L, and sodium chloride was 1.0g / L. Under the conditions of temperature 30°C, pH 11, and aerated culture, the bacteria grew and multiplied rapidly, and the growth curve is shown in Figure 4. It can be seen from Figure 4 that the bacterial growth retardation period is short and quickly enters the logarithmic growth period. The logarithmic period is about 6 to 16 hours, and then enters the stable period, and the number of bacteria is on the order of 109.

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Abstract

This invention provides a method for culture bacteria that can reduce high concentration Cr (VI) in basic medium. The bacteria are colorless Achromobacter sp. (CGMCC 1260) with a preservation name of Ch-1. this invention adopts nitrogen and carbon sources as the culture media, and culture at 25-25 deg.C, pH value of 7-11, and Cr (VI) concentration of 1-2000 mg / L. this invention can raise Cr (VI) reduction capacity to higher than 2g / L, and can be used in chemical plants or chromium salt treatment plants for large scale Cr (VI) reduction.

Description

technical field [0001] The present invention relates to a bacterium that reduces high-concentration hexavalent chromium in alkaline medium and its large-scale cultivation method, in particular to the biological treatment technology of alkaline chromium-containing waste water, chromium slag leachate and chromium slag from chromium salt factories and ferroalloy factories . Background technique [0002] At present, more than 100,000 tons of chromium slag are discharged every year in the country, and the accumulated stock has exceeded 4 million tons. The piled up chromium slag not only occupies a large amount of land, but also seriously pollutes groundwater due to soluble hexavalent chromium. In 2003, several serious chromium slags Pollution accidents are caused by the alkaline leachate of chromium slag. Alkaline chromium-containing wastewater or leachate of chromium slag has high pH (about 11), high concentration of Cr(VI), and high salinity. At present, there is a lack of good...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/025
Inventor 柴立元龙腾发彭兵何德文闵小波王云燕
Owner CENT SOUTH UNIV
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