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Degradative plasmid containing functional gene capable of degrading fluoranthene, and extraction method thereof

A technology for degrading plasmids and functional genes, applied in the field of microbiology and molecular biology, to achieve the effect of improving the biodegradation effect

Inactive Publication Date: 2013-12-11
BEIJING NORMAL UNIVERSITY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, there is a lack of research reports on PAH degradation plasmids at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Inoculate the Achromobacter sp. preserved in the General Microorganism Center of China Microbiology Culture Collection Management Committee into the glass bottle that has been added with fluoranthene and basal medium, trace metal liquid and vitamin c solution, and then culture the bottle at a temperature of Cultivate for 20 days in a shaker at 25-28° C. with a rotation speed of 100 r / min to obtain a cultured bacterial liquid. Centrifuge the bacterial solution at 10,000r / min for 10min, and add 10mL of NaH with a pH value of 9.0 and a concentration of 0.08M to the isolated bacterial cells. 2 PO 4 Buffer solution, vortexed for 5 minutes, then ultrasonically oscillated for 15 minutes, centrifuged at 10000r / min for 10 minutes, discarded the supernatant, added 7mL of solution A and mixed evenly. Then add 2.2 mg of N-acetylmuramic glycan hydrolase, shake at 0-4°C for 10 minutes, then add 1.5 mL of ethoxylated sodium alkyl sulfate solution with a concentration of 50 g / L, vorte...

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PUM

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Abstract

The invention provides a degradative plasmid containing a functional gene capable of degrading fluoranthene, and an extraction method thereof. The degradative plasmid containing the functional gene capable of degrading fluoranthene is obtained by extraction and purification from a bacteria liquid of Achromobacter sp. The degradative plasmid containing the functional gene capable of degrading fluoranthene is obtained by the steps of inoculating Achromobacter sp. in a glass bottle with fluoranthene, a basal culture medium, a trace metal liquid and a vitamin C solution; culturing for 20 days to obtain the bacteria liquid; and extracting and purifying the bacteria liquid. The degradative plasmid containing the functional gene capable of degrading fluoranthene can be used for building a strain having a degrading function for fluoranthene, increases biodegradation effect of polycyclic aromatic hydrocarbon pollutants represented by fluoranthene and has application potentials for soil and water polluted by polycyclic aromatic hydrocarbons.

Description

technical field [0001] The invention belongs to the technical field of microbiology and molecular biology, and specifically relates to a degradation plasmid containing a functional gene capable of degrading fluoranthene and an extraction method thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of toxic organic pollutants containing two or more benzene rings, which have strong carcinogenic, teratogenic and mutagenic effects. PAHs can cause great harm to the ecological environment and human health through bioaccumulation and food chain transmission, which has attracted great attention from environmental scientists in various countries. The treatment technologies for polycyclic aromatic hydrocarbons in the environment mainly include physical methods, chemical methods and biological methods, among which biological methods have the advantages of simple operation, low operating costs, and no secondary pollu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10
Inventor 豆俊峰秦伟丁爱中王营营杜勇超
Owner BEIJING NORMAL UNIVERSITY
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