Achromobacter sp. MT-H used for degrading di-2-ethylhexylphthalate

A technology of diisooctyl phthalate and achromobacter, which is applied in the field of biological treatment of environmental pollutants and can solve the problems that the degradation rate cannot meet the requirements of actual pollution control.

Active Publication Date: 2015-07-29
JINAN UNIVERSITY
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Problems solved by technology

However, the degradation rate of the reported strains to DEHP can not meet the requirements of actual pollution control, and there are few reports on the germplasm resources of Achromobacter that can degrade DEHP

Method used

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  • Achromobacter sp. MT-H used for degrading di-2-ethylhexylphthalate
  • Achromobacter sp. MT-H used for degrading di-2-ethylhexylphthalate
  • Achromobacter sp. MT-H used for degrading di-2-ethylhexylphthalate

Examples

Experimental program
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Embodiment

[0022] Example 1 Isolation and identification of strains

[0023] The return sludge of Datansha Urban Wastewater Treatment Plant in Guangzhou was collected, and 5 g of activated sludge samples were weighed in a 150 mL Erlenmeyer flask containing 50 mL of sterile water. Mud suspension was added to 100 mL containing DEHP (50 mg L -1 ) of the above MSM medium. After culturing at 30°C and 140 rpm for 7 days, the inoculum size of 1 mL was continuously enriched and transferred 10 times, and the content of DEHP in the medium was correspondingly increased to 1000 mg L -1 . Then the culture medium transferred 10 times was diluted 10 3 ~10 5 Double-spread on LB solid plates and incubate upside down at 30°C for 1-3 days. After a single colony grows on the plate, pick a single colony and streak it several times for purification, and obtain a strain of bacteria, numbered MT-H. Then the strains were inoculated on LB solid plates and cultured upside down at 30°C for 7 days, and th...

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Abstract

The invention belongs to the technical field of environmental pollutant biological treatment, and specifically discloses achromobacter sp. MT-H used for degrading di-2-ethylhexylphthalate (DEHP). The achromobacter sp. MT-H is preserved at China Center for Type Culture Collection (CCTCC) on 22th, Jan., 2015, and preservation number is CCTCC No.M 2015058. The achromobacter sp. MT-H possesses relatively high degradation efficiency on DEHP, and is capable of degrading DEHP preferably in a relatively large pH and temperature range. It is confirmed that MT-H can be applied on DEHP polluted biological treatment as an excellent DEHP degrading bacterium.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to a novel dioctyl phthalate degrading bacterium, and more specifically to a colorless bacterium degrading dioctyl phthalate ( Achromobacter sp.) MT-H. Background technique [0002] Phthalate, also known as phthalate, abbreviated as PAEs, is a general term for esters formed by phthalic acid. Among them, diisooctyl phthalate (DEHP) is a typical phthalate ester, which is widely used in people's daily life, especially as a plastic additive, which can reach 20-50% by weight . Since DEHP in plastic products is easily transferred into the environment, it widely exists in the atmosphere, water, soil and organisms. DEHP is the most toxic among phthalates. It can enter the organism through various channels, seriously interfere with its endocrine, reproductive and nervous systems, and has strong teratogenicity, mutagenicity, and carcinogenicity. A...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34B01D53/84B01D53/72C12R1/025C02F101/34
CPCA62D3/02A62D2101/28C12N1/205C12R2001/025Y02A50/20
Inventor 莫测辉赵海明李彦文蔡全英李慧杜欢向垒林静
Owner JINAN UNIVERSITY
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