Strain capable of degradation of benzo[a]pyrene

A technology of benzos and bacterial strains, applied in the field of biodegradation treatment, can solve the problems of few reports on microorganisms that can efficiently degrade high-molecular-weight PAHs, restrict development and application, and lack polycyclic aromatic hydrocarbon-degrading bacteria.

Inactive Publication Date: 2012-07-04
BEIJING NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the lack of efficient PAH-degrading bacteria restricts the further development and application of PAH-contaminated soil bioremediation technology.
Existing studies on the degradation of PAHs by microorganisms generally focus on the screening, isolation and purification of microorganisms that degrade low-molecular-weight PAHs, but there are few reports on microorganisms that efficiently degrade high-molecular-weight PAHs

Method used

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Examples

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Embodiment Construction

[0020] Add oil-contaminated soil to the open glass bottle with benzo[a]pyrene and basal medium to enrich and domesticate benzo[a]pyrene-degrading microorganisms, add benzo[a]pyrene, basal medium after 30 days The conical flasks of culture medium, trace metal solution, vitamin C solution and adsorbent are inserted into enriched and domesticated soil. Cultivate in an oscillator at a temperature of 25-30°C and a rotational speed of 100r / min for 5-7 days, then carry out cycle transfer, and after the number of cycles is more than 8 times, the degrading bacteria group that can efficiently remove benzo[a]pyrene can be obtained . Make the bottom plate of agar solid medium with benzo[a]pyrene as the only carbon source and energy source in the ultra-clean workbench, and make the top plate of agar solid medium containing benzo[a]pyrene degrading bacteria on the bottom plate, Culture the prepared medium plate in the incubator for 10-15 days. In the ultra-clean workbench, add benzo[a]pyr...

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Abstract

The invention discloses a strain capable of degradation of benzo[a]pyrene, and belongs to the technical field of biodegradation treatment. The benzo[a]pyrene degradation strain is identified as Achromobacter sp., and the strain preservation number is CGMCC3684. The strain is prepared into the bacterium suspension with the bacterium content of 1.25*10<8> CFU/ml. With the strain of the present invention, the degradation removal rates of the materials with the initial benzo[a]pyrene concentrations of 0.11 mg/L, 0.34 mg/L, 0.71 mg/L, and 1.19 mg/L are respectively 74.35%, 84.66%, 78.91% and 68.33%.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and particularly relates to a bacterial strain capable of degrading benzo[a]pyrene. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of toxic organic pollutants containing two or more benzene rings, which have strong carcinogenic, teratogenic and mutagenic effects. PAHs can cause great harm to the ecological environment and human health through bioaccumulation and food chain transmission, which has attracted great attention from environmental scientists in various countries. As early as the 1980s, the U.S. Environmental Protection Agency identified 16 unbranched PAHs as priority pollutants in the environment, and my country also included PAHs in the blacklist of environmental pollution. Biodegradation is one of the most effective means to restore PAHs polluted environment. [0003] In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/025
Inventor 程莉蓉豆俊峰丁爱中李帅冉杜勇超范福强
Owner BEIJING NORMAL UNIVERSITY
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