Strain capable of degradation of benzo[a]pyrene
A technology of benzos and bacterial strains, applied in the field of biodegradation treatment, can solve the problems of few reports on microorganisms that can efficiently degrade high-molecular-weight PAHs, restrict development and application, and lack polycyclic aromatic hydrocarbon-degrading bacteria.
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[0020] Add oil-contaminated soil to the open glass bottle with benzo[a]pyrene and basal medium to enrich and domesticate benzo[a]pyrene-degrading microorganisms, add benzo[a]pyrene, basal medium after 30 days The conical flasks of culture medium, trace metal solution, vitamin C solution and adsorbent are inserted into enriched and domesticated soil. Cultivate in an oscillator at a temperature of 25-30°C and a rotational speed of 100r / min for 5-7 days, then carry out cycle transfer, and after the number of cycles is more than 8 times, the degrading bacteria group that can efficiently remove benzo[a]pyrene can be obtained . Make the bottom plate of agar solid medium with benzo[a]pyrene as the only carbon source and energy source in the ultra-clean workbench, and make the top plate of agar solid medium containing benzo[a]pyrene degrading bacteria on the bottom plate, Culture the prepared medium plate in the incubator for 10-15 days. In the ultra-clean workbench, add benzo[a]pyr...
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