Active fermentation broth and application thereof

A fermentation liquid and active technology, applied to active fermentation liquid and its application field, can solve problems such as loss, crop yield reduction and agricultural production, and achieve the effect of simple preparation method

Inactive Publication Date: 2013-04-03
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Nematodes are one of the important infectious pathogens in agricultural production, which can reduce crop production and cause significant losses to agricultural production

Method used

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  • Active fermentation broth and application thereof
  • Active fermentation broth and application thereof
  • Active fermentation broth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Inoculate 100 μl of activated Achromobacter sp. CD-253 in liquid LB medium (50 ml medium in 250 ml Erlenmeyer flask), and culture at 30°C and 180 rpm for 96 hours. After the culture solution was centrifuged at 8000 rpm for 10 minutes, the supernatant was taken as a test sample, and the activity was tested by the liquid immersion method after being diluted ten times. The results are shown in Table 1.

[0019] Table 1 Activity of Achromobacter sp. CD-253 fermentation broth against M. incognita by liquid immersion method

[0020]

[0021] - no apparent activity

[0022] The results show that: the fermented liquid of the present invention has a strong poisonous effect on the root-knot nematode (M. incognita), and the lethality rate of the fermented liquid diluted 10 times to the root-knot nematode (M. incognita) reaches 24 hours. 98.42%. The 24-hour mortality of M. incognita in the medium control was 3.37%.

Embodiment 2

[0024] Basically the same as Example 1, the difference is that 250 ml Erlenmeyer flask was filled with 100 ml medium during fermentation, and cultured at 37° C. and 120 rpm for 96 hours. When testing the activity, no dilution is carried out, and the fermented stock solution is used for the liquid immersion test, and the tested nematode is Caenorhabditis elegans (C. elegans). The activity results are shown in Table 2.

[0025] Table 2 The activity of Achromobacter sp. CD-253 fermentation liquid on Caenorhabditis elegans (C. elegans) determined by liquid immersion method

[0026]

[0027] The results show that: the fermented liquid of the present invention has a very strong poisonous effect on Caenorhabditis elegans (C. elegans), and the lethality rate of the fermented stock solution to Caenorhabditis elegans (C. elegans) reaches more than 90% within one hour. The 24-hour mortality of C. elegans in the medium control was 0.82%

Embodiment 3

[0029] Basically the same as Example 2, the difference is that during fermentation, 250 ml of medium was packed in a 500 ml Erlenmeyer flask, and the fermentation supernatant cultured at 28°C and 120 rpm for 7 days was used as a test sample, and the test nematode was P. redivivus ). The activity results are shown in Table 3.

[0030] Table 3 Activity of Achromobacter sp. CD-253 fermentation broth against P. redivivus liquid immersion method (%)

[0031]

[0032] The results show that the fermented liquid of the present invention has a very good poisonous effect on P. redivivus, and the lethality rate of the fermented liquid for one hour to P. redivivus is over 90%. Mortality of P. redivivus in the medium control was 1.78% at 24 hours.

[0033] The strain and culture medium used in this experiment are exactly the same as those in the previous example, only the culture volume, temperature and time, and the test nematodes are different. In the previous example, 100 ml of me...

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Abstract

The invention provides an active fermentation broth and an application thereof, belonging to the technical field of biological pesticide. The active fermentation broth is obtained through the following steps: a, storing achromobacter sp. CD-253 with the preservation number of CGMCC No.6358 in an (LB) culture medium of yeast extract and tryptone (the yeast extract: 5.0g, the tryptone: 10.0g, sodium chloride: 10.0g, water: 1000ml, pH: 7.0); activating for 24 hours in the same liquid culture medium for use; b, inoculating 100ul of the activated strain into a triangular flask containing 50-250ml of the liquid LB culture medium, culturing for 96 hours under a condition with the temperature at 28-37 DEG C and the rotation speed at 120-180rpm to obtain culture broth, centrifuging the culture broth for 10 minutes at 8000rpm and then taking the supernatant to place in a refrigerator at 4 DEG C for later use, so that the active fermentation broth is obtained. The invention also provides the application of the active fermentation broth in preparing biological reagents for killing M. incognita, C. elegans and P. redivivus. The active matter provided by the invention has the advantages of low cost, convenient preparation, high efficiency and low toxicity.

Description

Technical field: [0001] The invention relates to an active fermented liquid and its application, belonging to the technical field of biological pesticides. Background technique: [0002] Nematodes are one of the important infectious pathogens in agricultural production, which can reduce crop yield and cause significant losses to agricultural production. At present, there are many methods for the control of nematodes, but the main method is chemical nematicides. Because the high toxicity and high residue of chemical nematicides pose potential threats to human health and the environment, biological nematicides have become a research direction for nematode control. Microorganisms are easy to cultivate and have a short growth cycle, so the use of microorganisms and their metabolites to control nematodes has attracted people's attention. [0003] Caenorhabditis elegans and Panagrellus redivivus are easy to cultivate and observe. They are large in size and sensitive to environme...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/025
Inventor 李国红卢皓张克勤王芯
Owner YUNNAN UNIV
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