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Achromobacter sp. capable of enduring zinc ion toxicity and application of Achromobacter sp.

A colorless bacillus and zinc ion technology, applied in the direction of bacteria, microorganism-based methods, biological water/sewage treatment, etc., can solve problems affecting the quality of effluent from urban sewage treatment systems, achieve huge economic and environmental benefits, and save equipment And the cost of investment, the effect of simplifying the process flow

Pending Publication Date: 2019-09-27
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the concentration of Zn(II) exceeding 10mg / L will affect the N in traditional denitrification. 2 O reduction process, denitrification rate and denitrification microbial activity are significantly inhibited, and at the same time, the microbial community structure is changed, thereby affecting the effluent quality of urban sewage treatment systems

Method used

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  • Achromobacter sp. capable of enduring zinc ion toxicity and application of Achromobacter sp.
  • Achromobacter sp. capable of enduring zinc ion toxicity and application of Achromobacter sp.
  • Achromobacter sp. capable of enduring zinc ion toxicity and application of Achromobacter sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Utilize scanning electron microscope to observe the apparent form of achromobacter in the presence of zinc ions

[0025] The high-efficiency aerobic denitrification strain used in this experiment is Achromobacter sp., which was isolated from the landfill leachate treatment system.

[0026] Inoculate Achromobacter sp. into 1L of LB medium (containing 5g of NaCl, 10g of tryptone, and 5g of yeast extract per liter) to prevent the invasion of miscellaneous bacteria and maintain the growth vigor of the bacteria for enrichment to cultivate. Centrifuge the cultured bacterial liquid, wash three times with 0.5% NaCl, and make the optical density OD 600 For 1-2 bacteria suspension. Then the bacterium suspension was inoculated respectively in the LB medium containing 25mg / L Zn(II) by 10% inoculum size, after 8h, the sample was removed by centrifugation at 8000rpm and 4°C for 5min to remove the supernatant, and washed with PBS (phosphoric acid saline buffer) and washe...

Embodiment 2

[0028] Example 2. Aerobic denitrification characteristics of Achromobacter in the presence of 10mg / L Zn(II)

[0029] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005gFeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0030] Achromobacter sp. was inoculated in BM medium containing 10mg / L Zn(II), and cultured with shaking at 30°C and 150rpm, and 100μL headspace gas was extracted every 5h with a valve injection needle. for the determination of N 2 O, 2 mL of gas was extracted with a sterile syringe and injected into a 2 L pure helium bag for the determination of NO. At the same time, 2 mL of bacterial suspension was extracted with a sterile syringe, and the bacterial liquid was centrifuged at 8000 rpm for 5 min at 4°C, and the supernatant was taken for ...

Embodiment 3

[0032] Example 3. Aerobic denitrification characteristics of Achromobacter in the presence of 25mg / L Zn(II)

[0033] Achromobacter sp. was inoculated in BM medium containing 25mg / L Zn(II) to test its aerobic denitrification performance. The result is as image 3 As shown, 100 mg / L nitrate nitrogen was utilized by the strain immediately after inoculation and was completely consumed within 25 h, with an average removal rate of 4 mg / L / h nitrate nitrogen. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 33.4mg / L at 15h, and then was reduced at 25h. N 2 O also accumulated gradually, reaching the highest value of 1.01mg / L at 20h, and then completely reduced at 45h. It must be pointed out that the highest NO accumulation in the intermediate product of the denitrification process was 83.1 μg / L at this time, accounting for only 0.083% of the nitrate nitrogen removal. It can be seen that the strain still has the ability ...

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Abstract

The invention relates to Achromobacter sp. capable of enduring zinc ion toxicity and application of the Achromobacter sp.. The invention provides the Achromobacter sp. capable of enduring zinc ion toxicity, and the preservation number of the Achromobacter sp. is CGMCC No. 2964. When the concentration of zinc ions in sewage is 0-50mg / L, the strain adopts nitrate nitrogen as a sole nitrogen source, and under an aerobiotic condition, the nitrate nitrogen removal rate is up to 100%, and the total nitrogen removal rate is as high as 100%. The strain has the advantages of being convenient and rapid to operate, high in denitrification efficiency, and the like, and has great significances for optimizing sewage biological treatment systems and alleviating influence of zinc ions upon sewage denitrification systems.

Description

technical field [0001] The invention belongs to the field of sewage biological treatment, and in particular relates to an achromobacter which can withstand the toxicity of zinc ions and simultaneously remove nitrate nitrogen in water under aerobic conditions and its application in sewage treatment. Background technique [0002] With the acceleration of urbanization and industrialization in our country and the continuous development of industrial production, a large amount of industrial wastewater is produced, which poses a great threat to human life and health. Since my country's industrial wastewater treatment capacity cannot keep up with the scale of industrial water use, some industrial wastewater is still discharged directly into the urban sewage pipe network without treatment. As an important industrial material, zinc ions are widely used in chemical industry, metallurgy, electroplating, tanning and coating industries. These industries will produce a large amount of zin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/16
CPCC02F3/34C02F2101/163C12R2001/025C12N1/205Y02W10/10
Inventor 陈倩倪晋仁高强孙卫玲刘思彤
Owner QINGHAI UNIVERSITY
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