32results about How to "Uniform reaction conditions" patented technology

The invention discloses a PIK3CA gene mutation site detection probe, a detection liquid phase chip and a detection method thereof. The PIK3CA gene mutation detection liquid phase chip mainly comprises a microball covered with a probe and a primer for amplifying a target sequence carrying a 9th exon and / or a 20th exon. The PIK3CA gene mutation detection liquid phase chip and the detection method can help simultaneously detect the sites with relatively higher frequency of the PIK3CA gene mutation, and can separately detect the 9th exon and the 20th exon or detect the 9th exon and the 20th exon at the same time, and the detection has uniform reaction condition, good specificity of detection results, high sensitivity, good accuracy up to 90% and short detection time.

The invention relates to a detecting deice, and in particular relates to a multi-index detecting device, a kit and an application thereof. When a plurality of indexes of a sample are detected in the prior art, detection reagents for different indexes are needed to use, and the samples are respectively added to reaction containers of the agents, so that the operation is very inconvenient. For solving the defect, the invention provides an individual multi-index detecting device. The detecting device comprises at least one multi-hole detecting strip, wherein at least two detecting holes are formed on the multi-hole detecting strip; each of the detecting holes comprises a lower part, a bottom part and an upper part; the bottom parts of the detecting holes are closed, the lower parts of the detecting holes are independent with each other; the lower parts and the bottom parts of different holes can be used for simultaneously covering different bioactive substances; and the upper parts of the detecting holes are communicated with each other. The multi-index detecting device provided by the invention can add the samples to a plurality of detecting holes by one-step sampling, so that a plurality of indexes of the samples are detected, and the operation is convenient and quick.

The invention provides an apparatus and method for preparing olefin from methanol, which comprises a reactor (1), a reactivator (6), a spent catalyst stripping stage (11), a reactor external catalyst cooler (12) and a reactivator external catalyst cooler (23), and the reactor (1) is internally equipped with a reactor charging distributor (2), a cold regeneration catalyst device external branched distributor (5), a reactor external catalyst cooler catalyst distributor (21), a reactor grating (3), a catalyst and reaction gas separator (4); the reactivator (6) is internally equipped with a reactivator main wind distributor (7), a reactivator grating (8), a spent catalyst device external branched distributor (9), and a regeneration catalyst stripping stage (10). The invention also provides a method for preparing olefin from methanol by using the apparatus for preparing olefin from methanol, and solves the problems of low reaction selectivity and catalyst runoff in the present technology for preparing olefin from methanol.

This invention discloses a test kit for detecting EGFR gene mutation associated with curative effects of non-small cell lung cancer. This invention also discloses a method for rapidly detecting EGFR gene mutation associated with curative effects of non-small cell lung cancer. The method is rapid and precise, and also has such advantages as easy operation, low requirement for apparatus, and high detection rate (higher than 90%).

The current is directed to a device comprising, a reaction chamber where a reaction takes place, one or more feeding chambers which contain feeding solution comprising chemicals required for the reaction, and one or more porous membranes separating the reaction chamber from the one or more feeding chambers, and wherein the one or more porous membranes are in substantially vertical position. The current invention is also directed to an apparatus comprising a plurality of devices, wherein each device comprises of a reaction chamber where a reaction takes place, one or more feeding chambers which contain feeding solution comprising chemicals required for the reaction, and one or more porous membranes which separate the reaction chamber from feeding chambers, wherein the one or more porous membranes are in substantially vertical position. These devices and apparatuses can be used for high throughput synthesis of biomolecules and chemicals and biological screening assays.

The invention discloses a method for batched formation of square beams for biological soil reinforcement. The method for batched formation of the square beams for biological soil reinforcement comprises the following steps that (1) materials required by a mold are prepared; (2) cut organic glass plates provided with holes are assembled; (3) a light mold required for tailoring of geotechnical cloth is manufactured, and the geotechnical cloth is tailored according to the sizes of light materials; (4) the geotechnical cloth is placed in square beam mold cavity units, and sandy soil is added into cavities defined by the geotechnical cloth; (5) prepared bacteria are poured into test piece molds; (6) the two or four wetted test piece molds are placed in a container side by side, and a plurality of layers of mold sets are arranged according to the actual condition; (7) the multiple layers of mold sets are soaked in a chemical solution; and (8) the square beams for biological soil reinforcement are formed after a reaction. According to the method for batched formation of the square beams for biological soil reinforcement, by means of the flatness of the surface of organic glass and the good water permeability of the holes, cuboid test pieces formed after the chemical reaction are angular, high in surface flatness, uniform in texture and stable in mechanical property, and a scientific research test for testing the bending performance can be conducted in the later stage conveniently.

The invention discloses a method for preparing anti-UV fabric by an in-situ method, belonging to the technical field of fabric post treatment. The method includes the steps of: weighing Ce (NO3)3.6H20, dissolving the Ce (NO3)3.6H20 in water, stirring to enable the Ce(NO3)3.6H20 to dissolve evenly so as to prepare Ce(NO3)3 solution with concentration of 1-5g / L; and adding a surfactant, aqueous ammonia and a strong oxidizer into the Ce(NO3)3 solution sequentially, stirring to be even, soaking the fabric in the solution for 10-120mins, taking out the fabric, and drying for 1-5mins at 80-180 DEG C. In this way, washable anti-UV fabric can be prepared. The weight ratio of the Ce (NO3)3.6H20, the surfactant, the aqueous ammonia and the hydrogen peroxide is 10:5-24:1-9:1-5; and the surfactant iscetyl-trimethyl ammonium bromide or dodecyl benzene sulfonic acid sodium salt. The method is simple in process and low in cost, and through the method, anti-UV fabric which can be tightly bonded withnanometer material can be prepared.

The invention relates to a large-scale continuous preparation device and method of metal nanoparticles, belonging to the technical field of metal nanoparticle preparation. The equipment includes a microfluidic injection pump, a microfluidic channel, a Y-shaped tee pipe, a heating plate system for temperature control of the reaction, and a temperature-controlled product collection pool; the channels of the present invention are parallel and straight, the structure is straight, and the solvent flow resistance is small. Solve the shortcomings of traditional micro-channel blockage; the platform has strong applicability, and can react with various solvents and metals; the reaction conditions are unified; segmental heating, multi-point precise control, and the ability of large-scale production.

The invention discloses a probe for detecting EGFR gene exon 20 mutation, a liquidoid chip and a detection method thereof. The liquidoid chip of the EGFR gene exon 20 mutation comprises a microsphere enveloped by the probe, the microsphere enveloped by a wild amido modification probe with base sequence selected from any of SEQ ID NO: 1-SEQ ID NO: 6 aiming at exon 20 and the microsphere enveloped by a mutant amido modification probe with the base sequence selected from any of SEQ ID NO: 7-SEQ ID NO: 12 aiming at exon 20. A space arm is used for connecting the base sequence and the amido of each probe. Every microsphere has different color codes. The liquidoid chip of the EGFR gene exon 20 mutation also comprises a primer used for amplifying a target sequence with exon 20 mutant site. The method provided by the invention is fast and exact, is simple for operating and improves the detection efficiency greatly.

The invention relates to a quick and efficient non-template agent hydro-thermal synthesizing method. The system can synthesize an alpha-molybdenum trioxide nanometer rod and a high-density echinoid molybdenum oxide based nanometer materials. Molybdenum peroxide acid prepared from molybdenum trioxide and aqueous hydrogen peroxide solution is used as a precursor, is produced into scattered alpha-molybdenum trioxide nanometer rod by hydro-thermal synthesis at a temperature of between 80 and 180 DEG C, and is produced into the peroxide modified molybdenum oxide hydrate by hydro-thermal synthesis at a temperature of between 65 and 75 DEG C. The hydrate is a multiscale structure; a nanometer thin slice, a micron prism and a nanometer rod-shaped structure unit are divergently assembled into a micron-size high-density echinoid structure. The hydrate is roasted to obtain high-density echinoid alpha-molybdenum trioxide. Modulation of the synthesizing condition can realize fine adjustment for appearance of the nanometer rod, the micron-size echinoid structure and the structure unit thereof. The method uses raw materials with low cost, has the advantages of simple technical process, controllable conditions and the like, and can promote research and application of the molybdenum oxide in the fields of sensors, field transmission, electrode materials and so on.

The invention discloses a nucleic acid probe, a liquid phase chip for detecting kRas gene mutation and a detection method thereof. The liquid phase chip for detecting the kRas gene mutation comprises a microsphere and a primer, wherein the microsphere is enveloped with a wild type and a mutation type probes which are modified with amidocyanogen and aim at kRas gene codons 12, 13 and / or 61; the primer is used for enlarging a corresponding mutant site which is enriched with the kPas gene codons 12, 13 and / or 61 and needs to be detected; and the tail end of the primer is provided with a biotin-labeled target sequence. The method is rapid, accurate and simple to operate, can synchronously detect a plurality of mutant sites in a hole and greatly improves detection efficiency. The liquid phase chip can be used for detecting the kPas gene mutation, can assist early diagnosis of pancreatic cancer, can accurately forecast effectiveness of molecular targeted drug treatment, is convenient for accurate medication in clinic and avoids the aging loss and the economic loss of unnecessary treatment.

Probes, liquidchip-based products, and methods for detection of EGFR gene mutation are disclosed. The liquid chip for detection of EGFR gene mutation mainly includes: microspheres coated with probes; primers for amplifying target sequence in exon 19 and / or exon 21.The liquid chip and methods can detect mutations with relatively higher frequency in EGFR gene, and detect mutations in exon 19 and 21 simultaneously or separately. The assay has high specificity and sensitivity with uniform reaction condition. Detection has over 90 percent accuracy and takes less time. The method can be applied not only to detection of EGFR gene mutations in tumor tissue samples but also to those in humour samples from cancer patients. Thus it can monitor dynamicly.

The invention relates to a conductive adhesive, in particular to a method for preparing a silver conductive adhesive by in situ synthesis. The method comprises the following steps of: dissolving silver nitrate in the mixed solution of ethanol and polyhydroxyl liquid organics; uniformly stirring to prepare a solution 1, wherein the concentration of mass amount of the silver nitrate is 1-4mol / L; adding epoxy resin into the solution 1 prepared in the step 1; uniformly stirring, and putting into a microwave oven to radiate for 2-4.5 minutes under microwave with the frequency of 2.45GHz and power between 320W and 480W to prepare a solution 2, wherein the addition amount of the epoxy resin is 20-30 percent of the weight of the solution 1; removing the ethanol in the solution 2, adding an anhydride curing agent and an anhydride curing accelerator into the solution 2; and uniformly stirring, and curing to prepare the conductive adhesive. The method solves the post-treatment problem in the nanometer preparation process, overcomes a series of defects, such as low yield and the like, has a uniform reacting condition compared with similar methods, and has a great progress compared with a two-step preparation method of a conductive adhesive.

The invention relates to a detecting deice, and in particular relates to a multi-index detecting device, a kit and an application thereof. When a plurality of indexes of a sample are detected in the prior art, detection reagents for different indexes are needed to use, and the samples are respectively added to reaction containers of the agents, so that the operation is very inconvenient. For solving the defect, the invention provides an individual multi-index detecting device. The detecting device comprises at least one multi-hole detecting strip, wherein at least two detecting holes are formed on the multi-hole detecting strip; each of the detecting holes comprises a lower part, a bottom part and an upper part; the bottom parts of the detecting holes are closed, the lower parts of the detecting holes are independent with each other; the lower parts and the bottom parts of different holes can be used for simultaneously covering different bioactive substances; and the upper parts of the detecting holes are communicated with each other. The multi-index detecting device provided by the invention can add the samples to a plurality of detecting holes by one-step sampling, so that a plurality of indexes of the samples are detected, and the operation is convenient and quick.