The invention provides a zinc negative electrode with a zinc ion conductivity interface modification layer, a battery and a preparation method, and belongs to the field of aqueous zinc battery metal zinc cathodes. The preparation method comprises: in air atmosphere, pre-constructing an interface modification material M on a base membrane; in a soaking environment by a wetting liquid, the interfacemodification material M layer on the base film being in close contact with the metal zinc to form the short-circuit primary battery, and the contacted interface modification material M and the metalzinc having spontaneous redox reaction, to make the interface modification material convert to ZnxM with zinc ion conductivity from M, meanwhile, transferring the interface modification material layerto the surface of the metal zinc negative electrode from the base film, and finally, obtaining the metal zinc negative electrode with the ZnxM interface modification layer with zinc ion conductivityon the surface. The ZnxM interface modification layer with the zinc ion conductivity can effectively inhibit dendritic crystal growth of a zinc negative electrode during charging and discharging of azinc battery, so that the interface stability of a metal zinc negative electrode is improved, and meanwhile, the cycling stability of a water-based zinc battery is improved. The method is simple and has a good actual effect.