The invention provides a preparation method of red pitaya wine. Fresh pitaya is selected, blanched, beaten, and deodorized with alcohol, and then extracted, coarsely filtered, clarified, diluted, and prepared to prepare the pitaya wine. The red heart dragon fruit wine prepared by the preparation method of the invention has bright color, harmonious fruit aroma and wine aroma, good flavor, suitable sweet and sour, and has a typical dragon fruit style. Moreover, the red pitaya wine prepared by the present invention has a higher betaine content, retains more betaine in the red pitaya, has anti-fatigue, improves immunity and other effects, and further improves the value of the pitaya.