83results about How to "Short elution time" patented technology

The invention relates to a method for extracting alpha-mangostin from a mangosteen fruit peel. The method comprises the following realization steps: (I) extracting the mangosteen fruit peel; (II) concentrating an extracting solution; mixing the extracting solution of every time and concentrating under reduced pressure to obtain a concentrated solution; (III) leaching the solution; (IV) carrying out silica gel column chromatography; (V) carrying out coarse crystallization; and (VI) carrying out recrystallization: adding a primary crystal into an organic solvent with the volume of 1 to 5 times to carry out thermal dissolution; placing the solution after finishing the thermal dissolution, reducing the temperature to a room temperature, carrying out recrystallization, and controlling the crystallization time to between 10 and 20 hours; and filtering and centrifugating a recrystallization liquid, drying the liquid in vacuum to obtain the 98 percent alpha-mangostin. The method has the advantages of simple process and short operation time.

The invention relates to a method of preparing spheroid polymer particles having a narrow size distribution by dispersion polymerization. This method comprises the steps of: providing a two-phase system in the form of a dispersion comprising an organic phase of droplets dispersed in an aqueous medium, mixing said organic phase in said aqueous medium under agitation without using a dispersion stabilizing agent to stabilize the dispersion, wherein the organic phase comprises at least a crosslinkable monomer, a polymerization initiator and an organic solvent for said monomer, and allowing the crosslinkable monomers to polymerize, while the two-phase system is agitated.

The invention discloses a purification and enzyme digestion transformation method of a recombinant human insulin precursor and belongs to the field of preparation of recombinant human insulin and analogues thereof. In the invention, fermentation supernatant of a secretorily expressed recombinant human insulin precursor is purified by using a cation exchange chromatography column, two different washing solutions are used during washing of chromatography, the elution of target proteins is performed more quickly, the elution time is shortened, the precipitation of the target proteins is reduced and the volume of eluted samples is reduced; an eluting solution with pH 7.0 to 9.0 is used during elution, phase change operation does not need to be performed to eluted products, the eluted products are directly added into trypsin for enzyme digestion transformation and the transformation efficiency is high. The method disclosed by the invention is simple to operate, the primary purification and phase change operation of the fermentation supernatant are completed through one step of chromatography purification, the purity of the samples can be improved from 14 percent to 95 percent, the purified samples are directly subjected to enzyme digestion, the enzyme digestion transformation efficiency is above 95 percent, the production process is simplified and the cost is saved.

The invention discloses a polypeptide purification method and belongs to the technical field of protein and polypeptide purification. A preparative HPLC (high performance liquid chromatograph) is used for purifying polypeptides, 2,2,2-trifluoroethanol or tetrahydrofuran is added to an organic phase of a mobile phase of the preparative HPLC, the elution gradient, flow velocity and detection wavelength are set, and high-purity peptides are obtained. The polypeptide purification method has the advantages that the elution time is shortened, the use quantity of an organic solvent is reduced, the purity is high, the separation effect is good, and the cost is saved.