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Method for determining capsaicin flexible liposome encapsulation rate using dextran microgel column

A technology of flexible liposomes and capsaicin, which is applied in liposome delivery, measuring devices, specific gravity measurement, etc., can solve problems such as capsaicin flexible liposome encapsulation efficiency not described, and achieve the goal of accelerating the determination of encapsulation efficiency Accurate speed, encapsulation rate and small error

Inactive Publication Date: 2009-01-14
GUANGDONG PHARMA UNIV
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  • Abstract
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Problems solved by technology

However, the literature does not describe the specific method of using dextran microgel column to measure the encapsulation efficiency of capsaicin flexible liposomes

Method used

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  • Method for determining capsaicin flexible liposome encapsulation rate using dextran microgel column
  • Method for determining capsaicin flexible liposome encapsulation rate using dextran microgel column

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0014] First use a centrifuge to remove the water in the dextran micro-column. The centrifuge speed is 1000 rpm, and the centrifugation time is 6 seconds; then add 0.4ml of capsaicin flexible liposome dropwise to the column, and centrifuge with a centrifuge. Elution liposome, centrifuge speed is 2000 revs per minute, centrifugation 5min, with disposable elution liposome as the condition, carry out the same batch of capsaicin flexible liposome 3 repeated tests and the test of 3 batch numbers (concentration Same), measure respectively the total content concentration of capsaicin in the flexible liposome and the content concentration of capsaicin (encapsulated) in the flexible liposome after the dextran microcolumn elution.

[0015] The determination method of the total content concentration of capsaicin in the flexible liposome is: draw capsaicin flexible liposome 0.4ml, add water 2ml, dilute to 10ml with dehydrated alcohol, take the water-ethanol of blank auxiliary material as c...

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Abstract

The disclosed method comprises: removing water in glucan microcolumn; adding the liposome on the column for centrifugation; measuring the total content concentration (C0) of capsaicam and the concentration of processed capsicine (C1); finally, obtaining the target value by calculating C0 / C1. This invention is fast and low cost.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to a method for measuring liposome encapsulation efficiency. Background technique [0002] Liposome encapsulation efficiency is an important evaluation index of liposomes, and the guiding principle of liposome preparation of Chinese Pharmacopoeia 2000 edition points out that liposome encapsulation efficiency must not be less than 80%. There are many methods for measuring the encapsulation efficiency of liposomes, including centrifugation, dialysis, gel column chromatography, ion exchange resin, ultrafiltration, etc. Commonly used methods include centrifugation, dialysis, and gel column chromatography. . The centrifugation method needs 40000 revs high-speed refrigerated centrifuge, the smaller the particle size, the larger the required rotating speed, and the longer the time, as reported by E.Touitou, the lipid vesicles of 150nm size, using 40000rpm, 3h can be combined with liposo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00G01N9/36A61K9/127A61K47/00
Inventor 龙晓英梁少华李力任林丹
Owner GUANGDONG PHARMA UNIV
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