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Method for preparing catechins

A technology of catechins and substances, which is applied in the field of preparation of catechins, can solve the problems of strong PVPP swelling performance, low product yield, and complexity, and achieve environmental pollution, high biological safety, and safety Guaranteed effect

Inactive Publication Date: 2010-04-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two important technical obstacles when PVPP is used as an adsorbent to remove impurities such as caffeine from tea extracts. One is that PVPP has a strong adsorption force to catechins, and ethanol, ethanol aqueous solution, acetone, acetone aqueous solution or Common eluents such as ethyl acetate cannot effectively elute the catechins adsorbed on PVPP, resulting in too slow and complicated elution process, low product yield, and difficult regeneration of PVPP; the other is PVPP has strong swelling performance, and the flow rate of ordinary chromatographic columns using PVPP as the adsorbent is very slow, which cannot meet the production and application requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, a kind of preparation method of catechin substance, carries out following steps successively:

[0041] 1) The tea extract purchased from Hangzhou Senna Tea Co., Ltd., the total amount of catechins in this extract is 61.2%, and the caffeine content is 10.3%. Dissolve the tea extract in an aqueous ethanol solution with a volume concentration of 20% at a solid-liquid ratio of 1 g / 25 ml, centrifuge at 4° C. and 6,000 rpm for 20 minutes, and collect the supernatant; obtain an aqueous solution of tea extract .

[0042] 2) Soak commercially available adsorbent PVPP and chromatography silica gel as a flow acceleration aid in 2 times the weight of DMSO for 12 hours respectively, pour off the floating fine particles, filter and recover DMSO through gauze mesh, and separate the processed PVPP and chromatography silica gel Wash repeatedly with a large amount of distilled water to remove the remaining DMSO, and then swell the washed PVPP and silica gel in distilled wa...

Embodiment 2

[0047] Embodiment 2, a kind of preparation method of catechin substance, carries out following steps successively:

[0048] 1) The tea extract purchased from Hangzhou Senna Tea Co., Ltd., the total amount of catechins in this extract is 46.3%, and the caffeine content is 6.7%. Dissolving the tea extract with 10% ethanol aqueous solution according to the solid-liquid ratio of 1g / 10ml, filtering under reduced pressure and collecting the supernatant to obtain the tea extract aqueous solution.

[0049] 2) Soak the commercially available adsorbent PVPP and the flow velocity acceleration aid microcrystalline cellulose in DMSO of twice the weight for 12 hours respectively, pour off the floating fine particles, and filter the gauze to recover DMSO; the processed PVPP and microcrystalline The cellulose was repeatedly washed with a large amount of distilled water to remove the remaining DMSO, and then the washed PVPP and microcrystalline cellulose were respectively swollen overnight in ...

Embodiment 3

[0054] Embodiment 3, a kind of preparation method of catechin substance, carries out following steps successively:

[0055] 1) The tea extract purchased from Hangzhou Senna Tea Co., Ltd., the total amount of catechins in this extract is 46.3%, and the caffeine content is 6.7%. Dissolve the tea extract with distilled water at 90° C. according to the solid-liquid ratio of 1 g / 20 ml, filter under reduced pressure (0.1 MPa) and collect the supernatant to obtain an aqueous solution of tea extract.

[0056] 2) Soak the commercially available adsorbent PVPP in 2 times the weight of DMSO for 12 hours, dump the floating fine particles, and filter the gauze to recover DMSO, then repeatedly wash the PVPP with a large amount of distilled water to remove the remaining DMSO, and then put it in Swell overnight in distilled water; use the same method to treat the flow rate acceleration aid mixed by chromatography silica gel and microcrystalline cellulose in a weight ratio of 1:1; mix the swol...

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Abstract

The invention discloses a method for preparing catechins which comprises the following steps of: (1), preparing a water solution of teat extracts; (2) preparing a chromatography column by mixing 1-3 parts of sorbent and 1 part of flow rate acceleration assistant by weight and then pouring the mixture into a column in a wet mode; (3) loading a sample: uniformly injecting the water solution of tea extracts into the chromatography column; (4) eluting impurities containing caffeine, wherein the effluent liquid is leacheate containing caffeine impurities; (5) eluting catechins: leaching a chromatography column bed again by an ethanol solution of dimethyl sulfoxide(volume concentration is more than or equal to 60%) or dimethyl sulfoxide as eluate, and collecting leacheate containing catechins; and (6) obtaining solid catechins. By adopting the method, the caffeine impurities can be effectively eluted, thereby obtaining the high-purity catechins.

Description

technical field [0001] The invention relates to a green preparation method that uses insoluble polyvinylpyrrolidone as an adsorbent, removes impurities from tea extracts by column chromatography, and efficiently recovers high-purity catechins. Background technique [0002] Catechins are important functional components of tea extracts, which have physiological functions such as quenching free radicals, anti-oxidation, anti-allergic, anti-aging, anti-radiation, anti-cancer, anti-bacterial and anti-inflammatory, etc., and are widely used in the fields of medicine and food. Broad application prospects. However, ordinary tea extracts contain a certain amount of impurities such as caffeine, and caffeine has adverse effects such as addiction, insomnia, and induced abortion, which limits the promotion and application of tea extracts in the fields of medicine and food. Therefore, the green preparation method of effectively decaffeinating and obtaining high-purity catechins from tea ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62B01D15/08
Inventor 陆建良董占波梁月荣叶俭慧郑新强范芳媛
Owner ZHEJIANG UNIV
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