The invention discloses a
stropharia rugoso-annulata strain breeding method. The breeding method comprises the following steps of preparing a
stropharia rugoso-annulata rejuvenation culture medium, inoculating
stropharia rugoso-annulata mother strains to the rejuvenation culture medium for variable-temperature rejuvenation
pure culture, and cultivating stress-resistant and high-activity strophariarugoso-annulata mother strains; preparing a shake-flask
liquid culture medium, inoculating the rejuvenated stropharia rugoso-annulata mother strains onto the shake-flask
liquid culture medium, and cultivating the stropharia rugoso-annulata shake-flask liquid strain by adopting a shake-flask liquid
submerged fermentation process; preparing a
fermentation tank
fermentation culture medium, inoculating the cultured shake-flask liquid strain onto the
fermentation tank fermentation culture medium, and cultivating a liquid
submerged fermentation strain of stropharia rugoso-annulata by adopting a fermentation tank liquid
submerged fermentation process. A composting fermentation method is adopted, the stropharia rugoso-annulata
cultivar culture medium is prepared, the prepared
cultivar fermentation culture medium is put into a
cultivar breeding tray hole, then a cultivated stropharia rugoso-annulata liquid submerged fermentation strain is inoculated to the cultivar fermentation culture mediumin the breeding tray hole through an
inoculation gun, and the stropharia rugoso-annulata cultivar is obtained through an open
solid fermentation process.