42 results about "Alpha mangostin" patented technology

The invention belongs to the field of natural product isolation and relates to a purification method of alpha-mangostin. The purification method comprises the following steps: 1) crushing mangosteen shells, adding 3-10 times of a 60-80% ethanol solution to soak and extract for 2-3 times, concentrating the extract while depressurizing, adding the concentrated solution into an aqueous two-phase solvent system for extraction, collecting the organic phase, and concentrating the collected organic phase to obtain a concentrate; 2) adding water to the concentrate for dispersion, adding the dispersed concentrate to a polyamide column for adsorption, carrying out gradient elution with an ethanol solution, detecting by thin-layer chromatography, collecting the eluate, concentrating the eluate while depressurizing, and standing for crystallization; and 3) filtering out the crystals, dissolving the crystals with a mixed solution, recrystallizing, and drying to obtain alpha-mangostin. The purification method of alpha-mangostin is simple in process and easy to operate, has high product purity, and is easy to enlarge production.

The invention belongs to the fields of innovative medicines and cosmetics and particularly relates to a preparing method and uses of mangostin shown as a formula (I) and mangostin analogues shown as a formula (II). Alpha-mangostin, beta-mangostin, gamma-mangostin and analogues thereof are respectively prepared through olefination and through controlling conditions for deprotection. According to the method, products are high in purity, operation is simple and convenient, yields are high, costs are low and the method is suitable for large-scale production. On one hand, the beta-mangostin, the gamma-mangostin, beta-methoxy-mangostin, and analogues thereof have ultraviolet absorption ability and ultraviolet light radiation preventing functions so that the beta-mangostin, the gamma-mangostin, the beta-methoxy-mangostin, and the analogues thereof can be adopted as a sun-screening agent separately or compounded with other sun-screening agents and applied into cosmetics; and on the other hand, the beta-mangostin, the gamma-mangostin, the beta-methoxy-mangostin, and the analogues thereof have activity of inhibiting acid sphingomyelinase so that the beta-mangostin, the gamma-mangostin, the beta-methoxy-mangostin, and the analogues thereof can be adopted as acid sphingomyelinase inhibitors and applied for preparation of medicines for preventing and treating acid sphingomyelinase related diseases mainly including cardio cerebrovascular diseases, neurological diseases, liver diseases, lung diseases, autoimmune diseases, infectious diseases and the like.

The invention relates to a method for extracting alpha-mangostin from a mangosteen fruit peel. The method comprises the following realization steps: (I) extracting the mangosteen fruit peel; (II) concentrating an extracting solution; mixing the extracting solution of every time and concentrating under reduced pressure to obtain a concentrated solution; (III) leaching the solution; (IV) carrying out silica gel column chromatography; (V) carrying out coarse crystallization; and (VI) carrying out recrystallization: adding a primary crystal into an organic solvent with the volume of 1 to 5 times to carry out thermal dissolution; placing the solution after finishing the thermal dissolution, reducing the temperature to a room temperature, carrying out recrystallization, and controlling the crystallization time to between 10 and 20 hours; and filtering and centrifugating a recrystallization liquid, drying the liquid in vacuum to obtain the 98 percent alpha-mangostin. The method has the advantages of simple process and short operation time.

The invention discloses a method for extracting alpha-mangostin from garcinia mangostana peel. The method includes the following steps that 1) the garcinia mangostana peel is crushed, reflux extraction is conducted once or twice with water of 8-10 times of weight, then reflux extraction is conducted for one to three times with a 90%-95% ethanol solution of 8-10 times of weight, an extracting solution of the ethanol solution is combined, the extracting solution is concentrated to be twice or three times of the weight of the garcinia mangostana peel, centrifuging is carried out, and after centrifugal residues are dried, a crude extract is obtained; 2) subcritical extraction is conducted by using the fluid which is formed by dimethyl ether and butane according to the weight ratio of 60-70 : 30-40 as an extraction agent, the extraction temperature is 68-72 DEG C, the extraction pressure is 2.8-3.2MPa, and the extraction time is 20-40min. According to the method for extracting the alpha-mangostin from the garcinia mangostana peel, water extraction and alcohol extraction are used for obtaining the crude extract first in the extracting process, the content of the alpha-mangostin of the crude extract is 70%-80%, then subcritical extraction is conducted, and the content of the alpha-mangostin is further made to reach 98%. The method is short in time, small in dosage of reagents, simple in step and high in yield and content, a subcritical extraction agent is volatilized directly to obtain a product, and thus the method is more suitable for industrial production.

The invention discloses novel medical application of Alpha-mangostin, i.e. application of Alpha-mangostin in preparing medicines for treating Alzheimer's disease. Under a brand-new working concentration, the Alpha-mangostin shows the characteristic of restricting A Beta accumulation and deposition, simultaneously has a neuroprotection role, can effectively resist a neurotoxin role caused by A Beta oligomer, strengthen the normal physiological function of neuron cells of mammals and maintain the normal cell form of the neuron cells of the mammals, realizes the intervention to the pathological course of the Alzheimer's disease and the improvement to the pathological symptoms and provides a new path for treatment of the Alzheimer's disease.

The invention provides an alpha-mangostin derivative and application thereof in preparation of a metabolic syndrome preventing and / or treating medicine. Through experimental proof, the alpha-mangostin derivative can simultaneously improve multiple metabolic disorders factors, and can obviously improve abnormal lipids metabolism, increase insulin sensitivity and reduce blood pressure. The compound provided by the invention has obvious treatment effect for the metabolic syndromes caused by gathering of the factors such as the abnormal lipids metabolism, blood pressure abnormity, blood glucose metabolic disorder and obesity and can be used as a metabolic syndrome clinic treatment medicine for use.

The invention discloses an application of alpha-mangostin in controlling plant diseases. Tests prove that alpha-mangostin has agricultural fungicidal activity. The compound has very strong fungistatic action on mango anthracnose pathogens, diplodina sp., botrytis cinerea, and exserohilum turcicum when the testing concentration is 100 mg / L, and has an EC50 value of 18 mg / L-35 mg / L; the compound has certain spore germination inhibition effect on exserohilum turcicum and botrytis cinerea, and the EC50 values are 66.20 mg / L and 50.43 mg / L respectively; meanwhile, in-vivo test results show that the compound has very good protection and treatment effects on rubber tree powdery mildew when the testing concentration is 500 mg / L, and the protection and treatment effects on rubber tree powdery mildew are 75.61% and 70.12% respectively, which are higher than those of a control drug of prochloraz. The above results indicate that alpha-mangostin has broad-spectrum fungistatic action on plant pathogenic fungi, which lays the foundation for the development of novel pollution-free plant-derived pesticides by further using mangosteen shells.

The invention relates to a preparation method of alpha-mangostin and the method has simple operation, low pollution and less energy consumption. The method comprises the following process steps: taking the shells of Garcinia mangostana Linn. to grind, adding the grinded shells in a CO2 supercritical extractor, using ethanol as the entrainer to obtain an extract; adding the extract in a polyamide chromatography column, using a mixed solvent of dichloromethane and methanol in a volume ratio of 8:1 as the eluting agent to perform elution, performing matching detection by drop a part of eluent on a polyamide film and using the eluting agent for developing, collecting the fluid phase which has 4-4.5 of Rf on the polyamide film, recycling the solvent, crystallizing, separating the crystals, recrystallizing with toluene, separating the crystals, washing and drying. By adopting the method of the invention to prepare alpha-mangostin, the product purity is high and the industrial amplification production is easy to realize.

The invention relates to a method for extracting alpha-mangostin from a mangosteen fruit peel. The method comprises the following realization steps: (I) extracting the mangosteen fruit peel; (II) concentrating an extracting solution; mixing the extracting solution of every time and concentrating under reduced pressure to obtain a concentrated solution; (III) leaching the solution; (IV) carrying out silica gel column chromatography; (V) carrying out coarse crystallization; and (VI) carrying out recrystallization: adding a primary crystal into an organic solvent with the volume of 1 to 5 times to carry out thermal dissolution; placing the solution after finishing the thermal dissolution, reducing the temperature to a room temperature, carrying out recrystallization, and controlling the crystallization time to between 10 and 20 hours; and filtering and centrifugating a recrystallization liquid, drying the liquid in vacuum to obtain the 98 percent alpha-mangostin. The method has the advantages of simple process and short operation time.

The invention provides an application of alpha-mangostin for prevention and treatment of diabetes mellitus. Alpha-mangostin can be used for decreasing blood glucose of diabetics, increasing glucose tolerance and improving symptoms of polydipsia, polyuria, polyphagia, emaciation and thirsty of the diabetics, can also be used for lowering high-fat-diet-induced obesity mouse total cholesterol content, has a cholesterol lowering effect, is capable of improving abnormality of the high-fat-diet-induced mouse glucose tolerance, and has functions of preventing and delaying obese patients from getting diabetes mellitus.

The invention discloses an alpha-mangostin derivative in preparation of an anti-cerebral palsy medicine and an anti-cerebral palsy medicine composition. The effective ingredient of the anti-cerebral palsy medicine composition is the alpha-mangostin derivative. By applying the alpha-mangostin derivative in the preparation of the anti-cerebral palsy medicine, the reduced learning and memory abilityof the brain caused by hypoxia ischemia and infection can be restored, the gait balance ability can be improved, and the damage of neuronal organelles can be relieved.

The invention discloses a hand sanitizer with an efficient sterilization function. The hand sanitizer with the efficient sterilization function comprises a surface active agent, a chelating agent, a wetting agent, a thickening agent and a bactericidal activity composition, wherein the bactericidal activity composition contains the compound MannolideA and alpha-mangostin. Due to the fact that the bactericidal activity composition contains the compound MannolideA and alpha-mangostin, mild sterilization can be achieved, and the sterilization effect is good.

The invention relates to a preparation method for plant extract, in particular to a method for separating and purifying alpha-mangostin from garcinia mangostana. According to the method, hydrophily-loaded organic carbon black is prepared and utilized for adsorbing garcinia mangostana bark powder, and the alpha-mangostin is extracted through the steps of acid precipitation, separating filtering and extracting. The method has the advantages that the extraction rate is high, and the purity of the extract reaches over 99.9%; the operation steps are simple, and the technological process is simplified; the needed cost is low.

The invention provides a cosmetic composition containing alpha-mangostin and use of the composition. It is verified that on one hand, alpha-mangostin has the ultraviolet absorption capacity, achieveshigh absorption in a UVB area of 290-320 nm and weak absorption in a UVA area of 320-400 nm and can complement other sunscreen agents; on the other hand, alpha-mangostin has the sunscreen synergisticeffect on some sunscreen agents, and therefore alpha-mangostin can be widely applied to cosmetics especially in sunscreen compositions.

The invention discloses application of alpha-mangostin to preparation of medicine for treating autoimmune disease. As proved by researches, the alpha-mangostin can be taken as an inhibitor of Th17 cell activity, and can be used for inhibiting differentiation and formation of Th17 cells.

The invention relates to a formula for treating and preventing arthritis, synovitis and other orthopaedic diseases and complicating diseases and discloses a main compound extracted from mangosteen:Garcinia mangostana. In the main compound, alpha-mangostin serving as a main ingredient is used in combination with various common filters in pharmaceuticals industry to prepare various proper formulations including tablets, capsules, powders, suppositories and the like for oral or external use for treating and preventing arthritis diseases such as rheumatoid arthritis, chronic infectious arthritis, post-traumatic arthritis, osteoarthritis and purulent arthritis as well as complicating diseases of the arthritis diseases such as inflammations of finger joints, metacarpophalangeal joints, wrist joints, elbow joints, shoulder joints, knee joints, spondyl joints, ankle joints, metatarsophalangeal joints and other joints. The alpha-mangostin content of the product of the invention is between 0.1 and 99.9 percent. The product manufactured by the formula can relieve the various inflammations and has certain analgesic effect. The product can be used alone or in combination with other substances.

The invention relates to a formula for treating prostatitis and other male reproductive system diseases. The invention discloses a main compound extracted from mangosteen: Garcinia Mangostana, wherein alpha-mangostin is taken as a main effective constituent and mixed with various commonly used fillers in the medicine industry to prepare various appropriate preparation formulations including tablets, capsules, powder, and suppository; and the various preparation formulations can be used for preventing and treating male reproductive system inflammations such as prostatitis, orchitis, deferentitis and urethritis by means of oral taking or external application. The content of the alpha-mangostin in the product is between 3 and 99.8 percent; and the prepared product can relieve various inflammations, has the function of relieving pain to certain degree, and can be independently used or mixed with other natural substances for use.

The invention discloses a method for extracting high-content alpha-mangostin from mangosteen skin. The method comprises the following steps: (1) extracting: crushing mangosteen skin raw materials, placing the crushed mangosteen skin raw materials into an extracting device, adding an acid alcohol solvent in amount which is 4 to 5 times the weight of the mangosteen skin raw materials, placing the solution into water bath at the temperature of 85-95 DEG C, extracting for 2 to 3 times and for 1.5 to 2 hours at each time, combining the extracting liquids and filtering to obtain the filtered extracting liquid; and (2) performing chromatography: allowing the filtered liquid to pass through a neutral alumina chromatographic column, performing gradient elution, collecting the eluent, concentrating the eluent under reduced pressure until no alcohol taste exists, standing the eluent at the temperature of 4 DEG C for more than or equal to 4 hours until crystallization, centrifuging, and taking the precipitate to obtain the finished product. The method has the advantages of few steps, low time consumption, little reagent dosage and low cost, and is simple to operate and easy to control. By the method, the alpha-mangostin with the highest content of more than or equal to 99.0% can be obtained. The method is suitable for industrialized production.

Methods of killing mosquito larvae and controlling an adult mosquito population by using α-mangostin, 3,6,8-yrihydroxy-2-methoxy-1,7-bis(3-methylbut-2-enyl)xanthen-9-one, or a salt, solute, hydrate or solvate thereof. Mosquito larvae are killed by contacting a lethal dose of α-mangostin with mosquito larvae. Adult mosquito populations are controlled by treating a mosquito larvae population with a lethal dose of α-mangostin.

The invention belongs to the field of chemical synthesis and particularly relates to a novel synthesis method of mangostin as shown in the formula (I), wherein the mangostin as a natural effective component has favorable anti-tumor activity, cardiovascular activity, antioxidant activity, anti-inflammatory activity, antibacterial activity and other pharmacological activities. The novel synthesis method comprises the steps: with 1, 7-dihydroxyl-3, 6-dialkoxyl-9H-xanthenone as a raw material, sequentially carrying out nucleophilic substitution, Claisen rearrangement, alkylation, deprotection and the like to obtain alpha-mangostin, beta-mangostin, belt-mangostin-OMe and gamma-mangostin. The novel synthesis method is simple in step and suitable for industrial production.

The invention relates to a mangosteen extract with alpha-mangostin, beta-mangostin and gamma-mangostin and a processing technique thereof, comprising the following steps: smashing, multi-level extracting, vacuum concentration, drying, etc. In the invention, ethanol is taken as extracting medium, the active ingredients of a whole mangosteen are extracted by a method of multi-level extracting and ethanol recovering, the produced mangosteen extract is powder, and the contents of alpha-mangostin, beta-mangostin and gamma-mangostin can be more than 14% and taken as food adhesives and accessories. The technique is characterized by simple technique, low cost and high content of mangostin in the extract.

The invention discloses a method for extracting alpha-mangostin from garcinia mangostana peel. The method includes the following steps that 1) the garcinia mangostana peel is crushed, reflux extraction is conducted once or twice with water of 8-10 times of weight, then reflux extraction is conducted for one to three times with a 90%-95% ethanol solution of 8-10 times of weight, an extracting solution of the ethanol solution is combined, the extracting solution is concentrated to be twice or three times of the weight of the garcinia mangostana peel, centrifuging is carried out, and after centrifugal residues are dried, a crude extract is obtained; 2) subcritical extraction is conducted by using the fluid which is formed by dimethyl ether and butane according to the weight ratio of 60-70 : 30-40 as an extraction agent, the extraction temperature is 68-72 DEG C, the extraction pressure is 2.8-3.2MPa, and the extraction time is 20-40min. According to the method for extracting the alpha-mangostin from the garcinia mangostana peel, water extraction and alcohol extraction are used for obtaining the crude extract first in the extracting process, the content of the alpha-mangostin of the crude extract is 70%-80%, then subcritical extraction is conducted, and the content of the alpha-mangostin is further made to reach 98%. The method is short in time, small in dosage of reagents, simple in step and high in yield and content, a subcritical extraction agent is volatilized directly to obtain a product, and thus the method is more suitable for industrial production.

The invention discloses an application of an alpha-mangostin derivative alpha-mangostin derivative in preparation of drugs for resisting and treating BPH (benign prostatic hyperplasia) and anti-BPH drug composition. The effective ingredient of the anti-BPH BPH drug composition is the alpha-mangostin derivative. The alpha-mangostin derivative can be absorbed by prostate tissue, still has anti-inflammatory activity, can improve the SOD activity and reduce MDA content and relieves enlargement of a prostate gland, therefore, the alpha-mangostin derivative can be used for treating BPH and has broad application prospects in preparation of the drugs for treating the BPH.

The invention relates to the field of traditional Chinese medicine and discloses novel application of Alpha-mangostin. The invention provides application of Alpha-mangostin in the preparation of drugs for improving islet cell functionality, insulin resistance and vascular endothelial functionality. Experiments show that Alpha-mangostin can improve islet cell functionality and insulin resistance, can improve vascular tissue form damage and endothelial cell edema, necrosis, shedding and structural distortion, and can reduce liver vacuolar denaturation. It is indicated that Alpha-mangostin can improve islet cell functionality, vascular endothelial functionality and fatty liver.

The invention belongs to the field of pharmaceutical preparations, relates to a novel medicine delivery system, and particularly relates to a medicine delivery system for regulating tumor microenvironment and active targeting and use thereof. The medicine delivery system adopts an FDA-approved biodegradable polymer polylactic acid material; a natural drug monomer alpha-mangostin (alpha-M) is physically occluded by an emulsion solvent evaporation method to prepare a medicine-loaded nano-delivery system (alpha-M NP); functional polypeptides are further modified on a surface of the alpha-M NP bycovalent binding; tumor-associated fibroblasts abnormally activated in the tumor microenvironment can be actively targeted so as to prepare a nano medicine delivery system with functions of active targeting and regulating the tumor microenvironment. In-vitro and in-vivo experiments show that the delivery system can inhibit excessive activation of the tumor-associated fibroblasts, effectively improves the tumor microenvironment, increases accumulation of medicines in tumor locations, and has greater clinical application prospects.

The invention discloses application of mangostin in prevention of bacterial wilt. The invention discloses that gamma-mangostin and alpha-mangostin have a good inhibition effect on Ralstonia solanacearum, and also discovers that after the Rastonia solanacearum is treated by the gamma-mangostin or the alpha-mangostin, the formation amount of Ralstonia solanacearum biological membranes is reduced, the length of a cell is obviously increased, the cell is deformed, a cell membrane is shrunk to different degrees, and the cell morphology is damaged, so that the growth and reproduction of the Rastoniasolanacearum are inhibited. Therefore, the gamma-mangostin and the alpha-mangostin can serve as an inhibitor or a pesticide preparation of the Rastonia solanacearum for preventing and treating agriculture-related diseases such as the bacterial wilt and the like, or the gamma-mangostin and the alpha-mangostin can serve as a soil additive and are added into soil for preventing and treating the bacterial wilt.

The invention relates to a preparation method of temperature-sensitive type chitosan-based anti-acne aquagel for cosmetics. The method is characterized by comprising the following steps of (1) preparing a chitosan water solution; (2) preparing a beta-sodium glycero-phosphate water solution; (3) under the 3-5 DEG C water bath condition, mixing the chitosan water solution and the beta-sodium glycero-phosphate water solution according to the mass ratio of (1 to 5):1 to obtain the chitosan / beta-sodium glycero-phosphate water solution; (4) preparing an alpha-mangostin ethanol solution; (5) preparing a genipin-aucubin water solution; (6) mixing the chitosan / beta-sodium glycero-phosphate water solution and the alpha-mangostin ethanol solution according to a mass ratio of (20 to 100):1 to preparethe alpha-mangostin-chitosan / beta-sodium glycero-phosphate water solution; (7) performing crosslinking on the alpha-mangostin-chitosan / beta-sodium glycero-phosphate water solution and the genipin-aucubin water solution according to the mass ratio of (100 to 200):1 at 78 to 80 DEG C to obtain the anti-acne aquagel. The preparation method has the advantages that the preparation process is simple; safety and nontoxicity are realized; the obtained anti-acne aquagel has a good anti-acne effect and high safety; the use is convenient, and the like.

The invention discloses a method for extracting high-content alpha-mangostin from mangosteen skin. The method comprises the following steps: (1) extracting: crushing mangosteen skin raw materials, placing the crushed mangosteen skin raw materials into an extracting device, adding an acid alcohol solvent in amount which is 4 to 5 times the weight of the mangosteen skin raw materials, placing the solution into water bath at the temperature of 85-95 DEG C, extracting for 2 to 3 times and for 1.5 to 2 hours at each time, combining the extracting liquids and filtering to obtain the filtered extracting liquid; and (2) performing chromatography: allowing the filtered liquid to pass through a neutral alumina chromatographic column, performing gradient elution, collecting the eluent, concentrating the eluent under reduced pressure until no alcohol taste exists, standing the eluent at the temperature of 4 DEG C for more than or equal to 4 hours until crystallization, centrifuging, and taking the precipitate to obtain the finished product. The method has the advantages of few steps, low time consumption, little reagent dosage and low cost, and is simple to operate and easy to control. By the method, the alpha-mangostin with the highest content of more than or equal to 99.0% can be obtained. The method is suitable for industrialized production.