The invention provides a
fermentation method of human intestinal
flora and application. The method comprises the following steps of a, donor screening, wherein boys who are twelve to fourteen years old, are in good health, do not have the serious
disease history or obvious
gastrointestinal tract symptoms, do not take any
antibiotics in at least six months and have a regular life are screened out;b,
sample collection and treatment, wherein fresh excrement of the donors is collected, quickly transferred to an anaerobic working
station and weighed, a pre-reduction
sterile saline solution is added according to a proportion, and even mixing with sufficient vortex is conducted; then filtering is conducted, and an excrement suspension solution is collected; c,
fermentation, wherein the excrementsuspension solution is transferred to a sterile serum
bottle, sealed and placed in a culture box for standing
fermentation. According to the fermentation method, the obtained pH-acidity fermentationproduct with multiple rich probiotics is a kind of optimized healthy human intestinal
flora and can be applied to the excrement bacterium
transplantation technology and development of medicines, health care products and the like with the human intestinal
flora as a target point.