The invention discloses a preparation method of a starter feed for scophthalmus maximus linnaeus fingerlings. The preparation method comprises the following steps of (a), mixing white fish meal with chicken meat powder, beer yeast, soybean meal, flour and a mineral element mixture to obtain a mixture, crushing the mixture, blending the crushed mixture, performing puffing granulation at 100-130 DEG C, and performing drying at the temperature of 50-100 DEG C; (b), mixing egg powder with wet artemia salina and shrimp mat, performing homogenating through a colloid mill, then adding betaine, fish oil, phospholipid and a vitamin mixture, and performing emulsifying and homogenating; and (c), under vacuum, performing vacuum-spraying of emulsifying liquid obtained in the step (b) onto the surfaces of semi-finished products namely baked particles obtained in the step (a), then performing drying, performing cooling, performing crushing, performing screening, performing weighing, and performing packaging. Bioactive components of fresh living feed raw materials and bioactive components of unstable nutrient elements in a feed formula can be effectively maintained, the food calling properties and the utilization efficiency of the feed can be improved, the survival rate of the scophthalmus maximus linnaeus fingerlings can be increased, the growth properties of the scophthalmus maximus linnaeus fingerlings can be improved, pathogens in the feed are reduced, and the water-quality environment of breeding water bodies is improved.