The invention belongs to the technical field of bioengineering technology, and particularly relates to a preparation method of an
oxygen consumption protective layer on the
chlorella cell surface. According to the preparation method of the
oxygen consumption protective layer on the
chlorella cell surface,
cell surface engineering technology is introduced to the green alga cell surface, green algacells generate
hydrogen in an atmospheric environment through single
coating of the green alga cell surface, and a functional protective layer is constructed manually on the cell surface to improve inherent nature and functions of a
living body. The preparation method of the
oxygen consumption protective layer on the
chlorella cell surface comprises the steps that step one, culture of
pyrenoid chlorella cells is carried out; step two,
pyrenoid chlorella cells modified by polydopamine are prepared; and step three,
pyrenoid chlorella cells coated with the polydopamine and
laccase are prepared. The oxygen consumption protective layer with a bi-component structure is constructed on the pyrenoid chlorella surface. The oxygen consumption protective layer has better
biocompatibility, can maintainown activity of the
living body, enables the periphery of the pyrenoid chlorella to maintain the atmospheric environment and generate
hydrogen, and has a certain defensive and protective effect on the
living body when the external environment is unfriendly.