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Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof

A technology of HAU-M1, photosynthetic bacteria, applied in bacteria, microorganism-based methods, and adding compounds to stimulate growth, etc., can solve problems such as low hydrogen production efficiency

Active Publication Date: 2019-06-25
HENAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current problem is that when corn stalks are used as substrates for synchronous saccharification and fermentation to produce hydrogen, the hydrogen production efficiency of HAU-M1 photosynthetic bacteria is low

Method used

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  • Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof
  • Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof
  • Medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof

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Experimental program
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Effect test

Embodiment

[0036] In order to obtain a medium that can improve the hydrogen production capacity of HAU-M1 photosynthetic bacteria, the applicant chose to change the amino acid composition in the medium to investigate the hydrogen production changes during the fermentation process. The added amino acids were L-cysteine, L- One of leucine, L-alanine, L-threonine, L-serine;

[0037] In order to comprehensively investigate the effect of amino acid concentration on the hydrogen production capacity of HAU-M1, the applicant conducted a comparative experiment under different conditions for different amino acids. Among them, when adding L-cysteine, L-alanine and L-threo When L-leucine is added, the added concentrations are 0.3 g / L, 0.6 g / L, 0.9 g / L, 1.2 g / L; when L-leucine is added, the added concentrations are 0.6 g / L, 0.9 g / L , 1.2 g / L, 1.5 g / L; when L-serine was added, the added concentrations were 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L; the control group did not add hydrogen-producing amino acids; E...

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Abstract

The invention belongs to the technical field of microbial fermentation biological hydrogen production, and particularly relates to a medium for promoting hydrogen production of HAU-M1 photosynthetic bacteria flora and application thereof. The medium comprises 0.1-1g / L of NH4Cl, 0.01-0.5g / L of MgCl2, 0.01-0.2g / L of yeast extract, 0.1-1g / L of K2HPO4, 1-3g / L of NaCl, 1-5g / L of sodium glutamate and 0.3-2.5g / L of hydrogen-producing amino acid. The hydrogen-producing amino acid is one or a mixture of a plurality of L-cysteine, L-alanine, L-threonine, L-leucine and L-serine in any ratio. Based on a general inventive concept, the invention also provides application of the medium in biological hydrogen production, thereby effectively improving the hydrogen production capability of the HAU-M1 photosynthetic bacteria flora.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation biological hydrogen production, and in particular relates to a culture medium for promoting hydrogen production of HAU-M1 photosynthetic bacterial flora and its application. Background technique [0002] Hydrogen energy is considered to be the most ideal alternative energy source in the future because of its high combustion calorific value, cleanness and pollution-free characteristics. Among many hydrogen production technologies, biological hydrogen production has attracted more and more attention because of its high efficiency, greenness and low cost. . Research on biological hydrogen production at home and abroad mainly focuses on two aspects: anaerobic dark fermentation hydrogen production and photofermentation hydrogen production. Among them, photosynthetic biological hydrogen production technology uses photosynthetic bacteria to convert straw, sludge, and feces as raw materials...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P3/00C12N1/38C12N1/20C12R1/01
CPCY02E50/30
Inventor 张全国夏晨曦杨雪梅路朝阳胡建军王艳锦王毅张志萍
Owner HENAN AGRICULTURAL UNIVERSITY
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