Invention performs an
assay to determine presence or quantity of specific
analyte in fluid sample. Representative device has two separate flow paths established sequentially in device with a single user activation step. First flow path delivers sample, and conjugate soluble binding reagents to
solid phase. If
analyte is present, an
analyte:conjugate complex is formed and immobilized.
Sample volume delivered by first path determined by absorbent capacity of
solid phase, and not by amount of sample added to device. User need not measure
sample volume. Sample / conjugate mixture is prevented from entering second flow path because capillary and
surface energy of second flow path prevent it from being wetted by this mixture. Second flow path allows wash
reagent to remove unbound conjugate and sample from
solid phase to the absorbant, and optionally deliver detection reagents. Adaptable for many formats including, sandwich immunoassays,
colloidal gold,
sol particle, heterogeneous generic capture, and competitive assays.