33results about How to "Long synthesis time" patented technology

The invention discloses a method for producing phospholipase D through recombinant escherichia coli. According to the method, a phospholipase D gene is in preciseness-type starting sub control, a pararea of pSC101 is inserted in an expression plasmid, an escherichia coli mutant strain recA is used for keeping the genetic stability of the plasmid, cells containing the plasmid at the growth periodare cultured to high density, and saturation induction is conducted at the induction period; meanwhile, the temperature is decreased, alkali metal salt stress is applied, the cytotoxicity of PLD to ahost is reduced, cell lysis is inhibited, the synthesis time of PLD is prolonged, and accordingly PLD expression is improved.

The invention discloses a production method of tartary buckwheat sprouts, which comprises the steps of disinfection, germination acceleration, seed soaking, intermittent microwave treatment, alternate dark light cultivation, etc., and uses salt stress to significantly increase the germination rate of seeds and promote flavonoids in sprouts The synthesis and enrichment of rutin and other flavonoids are beneficial to the synthesis and enrichment of flavonoids such as rutin, and the germination rate of tartary buckwheat seeds is improved by using intermittent microwave treatment to effectively avoid enzyme inactivation caused by high temperature. The content of flavonoids and antioxidant capacity; the allelopathic effect of the water extract of Artemisia annua can inhibit the growth of tartary buckwheat sprouts, prolong the time from germination to harvest, and increase the synthesis time of beneficial substances. Sprouts synthesize a large amount of flavonoids per unit time, which significantly increases the total content of beneficial substances in sprouts. After inhibiting the growth of root hairs, the workload of root hair removal can be reduced, the proportion of harvestable parts can be increased, and production efficiency can be increased.

The industrial preparation method of silicon carbide whiskers and micrpowder is characterized by that the graphite and other industrial carbon and silicon raw materials are mixed, and placed into industrial SiC smelting furnace to make synthesis reaction, the graphite is used as conductive heating body to prepare silicon carbide whiskers and micropowder. Said preparation method is simple, the diameter of whiskers produced by said invented method is uniform, and length-diameter ratio is large and the heteromorphous crystal is less, and product quality is high.

The invention discloses fully synthetic cutting fluid with high abrasion resistance and high antirust performance and a method for preparing the fully synthetic cutting fluid, and belongs to the technical field of technological lubrication. By the aid of the fully synthetic cutting fluid and the method, the problems of poor universality, short user cycles, vulnerability to decaying and deterioration and severe pollution of the traditional cutting fluid mainly can be solved. The fully synthetic cutting fluid comprises, by weight, 0.50%-1.00% of disodium tetraborate decahydrate, 1.00%-2.00% of extreme-pressure agents, 0.02%-0.10% of defoaming agents, 0.08%-0.15% of 1, 2, 3-benzotriazole, 0.50%-1.50% of polyethylene glycol, 1.00%-2.00% of synthetic additive components A and the balance deionized water. The synthetic additive components A in the fully synthetic cutting fluid are multi-effect additives, and oleic acid and triethanolamine are synthesized according to a mass ratio of 1.8-2.0under the condition of constant temperatures ranging from 60 DEG C to 80 DEG C for 3-6 h to obtain the multi-effect additives. The fully synthetic cutting fluid and the method have the advantage thatthe fully synthetic cutting fluid not only is applicable to common cutters, but also is applicable to hard aluminum alloy cutters, can be used for machining diversified materials at high and low speeds and particularly can be used for machining cast iron, carbon steel, stainless steel and the like at high and low speeds.