A method for acidification formation of a long-life lead storage battery is disclosed, the method includes the following steps: 1, adding acid to a lead storage battery for the first time, adding lowspecific gravity sulfuric acid solution with density of 1.05-1.25 g / cm3 to lead storage battery to be acidified, the adding volume being 90-105% of saturated acid absorption capacity of the lead storage battery; 2, formation in the first stage, charging, wherein the total char electric quantity is 3.5 C - 5.0 C; (3) adding acid for the second time, adding high specific gravity sulfuric acid solution with density of 1.25-1.65 g / cm3, and filling; (4) forming in the second stage, first discharging and then charging, the depth of discharging is 25% - 80%, the total charge quantity is 3.5 C - 5.0 C; (5) Extraction of residual acid. As the acid is added in two times, the specific value of the positive electrode active material alpha-PbO2 / beta-PbO2 can be effectively improved, the service life ofthe battery is prolonged, the polarization can be effectively reduced, the formation efficiency can be improved and the formation period can be shortened.