118results about How to "Improve Microecology" patented technology

The invention relates to the field of soil remediation and discloses an in-situ bioremediation preparation for heavy metal contaminated soil. The preparation contains fungi and/or bacteria with heavy metal degrading abilities, the fungi refer to at least one of aspergillus niger, rhizomucor variabilis and grey cunninghamella, and the bacteria refer to at least one of bacillus amyloliquefaciens, bacillus licheniformis and bacillus simplex. By using the preparation for in-situ bioremediation of the heavy metal contaminated soil, the problems that the soil needs to move by using a moving method and secondary chemical agent pollution of the cultivated land is easily caused by using a decomposition method can be overcome, at the same time, residual heavy metals in the soil obtained after catabolism through the fungi and/or bacteria can also be fully used, the problem that high residual heavy metals in the soil seriously endanger food safety is solved, the soil micro-ecology is improved, the cultivated ability of soil is more quickly recovered, and the method is relatively low in cost and has broad application prospects.

The invention discloses a bacillus subtilis HL-1 and an application thereof in the respect of soil phosphate dissolving. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on Aug 24th, 2011, with a collection number of CGMCC No.5175. The 16SrDNA nucleotide sequence of the strain is represented by SEQ ID NO:1. With added diatomite and guava slag adsorbing agent materials, a fermentation broth of the bacillus subtilis HL-1 can be prepared into microbial manure. With the strain provided by the invention, phosphate availability and phosphate fertilizer utilization rate of the soil can be improved, plant growth can be promoted, and soil microbe diversity can be improved. The strain has important significance in reducing chemical fertilizer application, modifying soil micro-ecology, and promoting soil sustainable utilization. The strain has wide application prospect in production practice.

The invention utilizes a microbial fermentation technology to improve the forage nutritional value of the cottonseed meal, so as to improve the forage quality of the cottonseed meal, increase the addition proportion in the forage and realize the promotion of the protein nutritional value and the feeding value of the cottonseed meal, aiming at the disadvantages that cottonseed meal is rich in antinutritional factors of free gossypol, cellulose, oligosaccharide, phytic acid and the like, the protein quality is relatively poor and the content of essential amino acids of methionine and the like is low and unbalanced, so that the large-scale forage of cottonseed meal is restricted. The method for improving forage nutritional value of cottonseed meal through the microbial fermentation adopts the technology of 'composite enzymatic hydrolysis and multi-strain three-step solid-state fermentation', so as to prepare the fermented cottonseed meal product that is high in the free gossypol, cellulose degradation degree and the protein content; the cottonseed meal product is rich in nutritional ingredients of probiotics, vitamins, enzyme, amino acid, short peptide, high-grade protein and the like; the method for improving the forage nutritional value of cottonseed meal through the microbial fermentation has the advantages of prompting growth and development, improving the utilization ratio of the forage, enhancing immunization, improving intestinal micro ecology, preventing diseases and the like. The method for improving forage nutritional value of cottonseed meal through the microbial fermentation adopts the solid-state fermentation; the manufacturing technology is simple; the energy consumption is low; zero environmental pollution is generated; and the investment is small. Therefore, mass production is easy.

The invention discloses konjac oligomerization mannose sweet potato vinegar and a preparation method thereof. The konjac oligomerization mannose sweet potato vinegar is prepared from the following raw and auxiliary materials in weight ratio: 10-20 parts of konjac, 100-150 parts of sweet potatoes, 8-12 parts of bran, 16-23 parts of husk, 0.02 part of alpha-amylase, 0.015 part of calcium chloride, 0.3 part of saccharifying enzyme and 0.1 part of magnesium chloride. The preparation method comprises the following steps: (1) hydrolyzing the konjac by using aspergillus niger; (2) uniformly mixing steamed sweet potatoes and konjac hydrolysate, liquefying the mixture by using the alpha-amylase, saccharifying the mixture by using the saccharifying enzyme, and hydrolyzing cellulose and pectin by using the aspergillus niger to obtain saccharification liquor; (3) inoculating lactobacillus fermenti, brewer yeast and aroma-producing yeast into the saccharification liquor, fermenting at low temperature, and standing to obtain alcoholization liquor; (4) mixing the bran, the husk and the alcoholization liquor to carry out acetic fermentation, spraying vinegar, and ageing to obtain the konjac oligomerization mannose sweet potato vinegar. The konjac oligomerization mannose sweet potato vinegar prepared by using the method has the characteristics of mild sourness, remarkable sweet potato flavor and capability of relaxing bowels.

The invention discloses an enteric coated fodder acidifying agent which comprises the following ingredients by weight: 8 to 20 parts of a citric acid, 8 to 20 parts of a fumaric acid, 7 to 15 parts of a benzoic acid, 5 to 20 parts of a malic acid, 3 to 15 parts of a formic acid, 2 to 10 parts of an acetic acid, 6 to 15 parts of a propanoic acid, 2 to 5 parts of a butyric acid, 10 to 20 parts of an emulsifier, and 15 to 50 parts of an enteric slow-release coating material. Meanwhile, the invention discloses a preparation method of the enteric coated fodder acidifying agent. The preparation method comprises the following steps: preparation of an acidifying agent suspending liquid, preparation of an acidifying agent emulsion liquid, fluidization of the acidifying agent emulsion liquid, atomization of the enteric slow-release coating material, preparation of the enteric coated fodder acidifying agent, and the like. The enteric coated fodder acidifying agent prepared according to the preparation method has the advantages that the product purity is high; the enteric coated fodder acidifying agent is released slowly only after arriving the rear segment of an animal intestinal canal, so as to effectively play the bacteriostatic role and improve animal alimentary canal micro-ecology.

The invention discloses a fermented feed for dairy cows, which is prepared from raw materials of wheat straw and distillers' grains, is produced by using microbial fermentation and is high in content of proteins, strong in palatability and rich in saccharomyces cerevisiae, bacillus subtilis and other probiotics. In the product, the content of true proteins is about 42-48%, the content of the proteins is improved by above 12%, and the fermented feed further contains a large number of beneficial live bacteria, a variety of vitamins, enzymes, amino acids, short peptides, high-quality proteins and other nutritional ingredients. The fermented feed disclosed by the invention has the advantages that 1) through the fermentation enzymolysis effect of microbes, the content of the true proteins in the product is improved, thereby being conductive to making up for the blank in the protein feed for farming the dairy cows in China; 2) the product is rich in the nutritional ingredients and has the advantages of promoting growth and development of the dairy cows, increasing the utilization rate of the feed, enhancing the immunity, improving intestinal microecology, preventing diseases, reducing environmental pollution, increasing the milk production rate and the like; and 3) the solid-state fermentation is adopted, so that the production process is simple and environmental pollution is avoided.

Relating to the soil remediation field, the invention discloses a preparation for in-situ remediation of chlorothalonil contaminated soil. The preparation contains fungi and/or bacteria with chlorothalonil degradation ability. The fungi can be at least one of Aspergillus niger, rhizomucor variabilis and Cunninghamella bertholletiae, and the bacteria can be at least one of Burkholderia zhejiangensis, pseudomonas aeruginosa and Stenotrophomonas maltophilia. According to the invention, the provided preparation is employed for in-situ bioremediation of chlorothalonil contaminated soil, not only overcomes the problems that photochemical degradation is easily limited by illumination conditions and can easily cause secondary chemical reagent pollution to arable land, at the same time makes full use of fungi and/or bacteria for catabolism of chlorothalonil residual in soil, thus solving the problem that high residual chlorothalonil in soil severely endangers food safety. At the same time, the micro-ecology of soil is improved, so that soil can recover farming ability more rapidly. Also, the method is low in cost, thus having broad application prospects.

The invention discloses application of an organic compound. The technical characteristic is as follows: the application objects of triglyceryl lactate as a feed acidifier include monogastric animals such as pigs, poultry and the like, ruminants such as cows, sheep and the like, herbivores such as horses, rabbits and the like or fishes, shrimps and crabs. The application method comprises the following steps of: directly adding 0.05-1.0% of the triglyceryl lactate into the feed based on the total weight of the feed; or mixing the triglyceryl lactate with an adsorbent to obtain a premix; or mixing the triglyceryl lactate with other feed additives or feed raw materials to obtain a premix or a concentrate to feed monogastric animals such as pigs, poultry and the like, ruminants such as cows, sheep and the like, herbivores such as horses, rabbits and the like and aquatic animals such as fishes, shrimps, crabs and the like. Through the invention, the triglyceryl lactate is slowly decomposed in the alimentary canals of animals while releasing the characteristics of lactic acid, the pH value of the gastrointestinal tract is reduced, the growth of harmful bacteria in the gastrointestinal tract is suppressed, the reproduction of beneficial bacteria is promoted, and an effect of improving gastrointestinal microecology of animals is realized so as to promote the animal health and improve the production performance.

An ecological planting rope of straw belongs to the field of water-saving and no-tillage agriculture, that the straw which is hard to be braided into straw rope is crushed into straw powder of Phi1-5mm, then mixed with straw zymocyte species and plant seeds uniformly to form a inner layer of the rope body. The mixture of the inner layer of the rope body is uniformly mixed with slow-release matetials for keeping water and fertilizer by absorbing water, water-absorbing materials and algae, which adheres tightly to the inner layer of the rope body to form a interlayer of the rope body. Otherwise, a uniformly rope body is formed by mixing the combination of the inner lay and the interlayer of the rope body renewedly. Outer layer of straw rope is braided with sraw materials to form the ecological planting rope of straw finally outside of the rope body, which has a cross-section of basically round, and a diameter for 10-50mm. Integration operation of sowing, watering, water-keeping, fertilize-keeping, soil condition of soil manure improvement and no-tillage planting, is achieved by using special sowing equipment for the planting rope, which has a function of field protection, ecological environment improvement, and ensuring traditional agriculture and forestry to be transformed into modern agriculture and forestry.

The invention provides a bacteriostatic and mite-removing shampoo and a preparation method thereof, and relates to the field of daily chemicals. The bacteriostatic and mite-removing shampoo is prepared from the following raw materials: sodium laureth sulfate, decyl glucoside, behenamidopropyl dimethylamine, cocamide MEA, Polyquaternium-52, Cocamidopropyl betaine, ziziphus joazeiro bark extract, Piroctone ethanolamine salt, Gentian extract, guar hydroxypropyl trimethyl base ammonium chloride, polyquaternary ammonium salt-10, Cnidium Monnieri fruit extract, dimethiconol, amino-terminated Polydimethylsiloxane, plant extract, nicotinamide, etidronic acid, Carbomer, sodium hydroxide, preservatives, flavors, hexamidine bis (hydroxyethanesulphonic acid) salt, menthol and water, wherein the plantextracts are clove bud extract, litsea eubeba extract, thyme extract and lightyellow sophora root extract. The shampoo obtained through the mutual matching of the components and the optimization of the preparation method has good antibacterial and mite-removing effects.

Belonging to the technical field of agriculture, the invention specifically provides a compound long-acting slow-release organic fertilizer with animal dung as the main raw material and diatomite as the main auxiliary material. The compound long-acting slow-release organic fertilizer is mainly prepared from 55-65 parts by weight of biogas residue obtained by pig manure anaerobic fermentation, 20-25 parts by weight of diatomite, 10 parts by weight of edible fungus rod waste, 5-6 parts by weight of a fermentation inoculum, and 10-20 parts by weight of harmless treatment products of pigs dying of illness. The compound long-acting slow-release organic fertilizer provided by the invention contains rich silicon and other elements needed by crop growth, and has more comprehensive nutritional ingredients. Especially through effective compounding of all the raw materials, the effect of long-term slow-release of fertility is achieved. On the basis, the compound long-acting slow-release organic fertilizer also has the effects of maintaining moisture, loosening soil and improving the soil fertilizer conservation performance.

The invention discloses a feed for piglets with reduced serum lipopolysaccharide level and its preparation method and application, which solves the problem that the disease resistance and immunity of piglets cannot be improved in the prior art. The invention is made of the following components according to a certain weight ratio: corn, puffed corn, broken rice, puffed soybean, fermented soybean meal, fish meal, soybean meal, soybean oil, stone powder, calcium hydrogen phosphate, sodium chloride, choline chloride, betaine , lysine, methionine, threonine, tryptophan, benzoic acid, phytase, compound enzyme, trace element premix, vitamin premix, enramycin premix, arginine, glutamine Amide, proline, astragalus polysaccharide, eleuthero polysaccharide, curcumin, chitosan oligosaccharide, probiotics, yeast cell wall extract. The composition of the invention is scientific, the compatibility is reasonable, each component acts together, can provide balanced and easily digestible nutrients for the growth and development of piglets, and effectively reduce the level of serum lipopolysaccharide of piglets.

The invention provides a liquid oral composition containing TCM active substances and the tea tree essential oil. The liquid oral composition comprises the following materials in parts by weight: 1.0 to 10 parts of traditional Chinese medicine extracts, 0.1-6 parts of additives, 0.001-0.005 part of menthol, 0.001-0.006 part of tea tree essential oil, and 90-100 parts of water. The traditional Chinese medicine extracts are composed of green tea extract, affine cudweed extract, plantain herb extract, Lamium album extract, tea tree root extract, gallnut, honeysuckle and orange peel mixed extract, and violet extract. The additives are composed of a wetting agent, a pH regulator, a preservative, essence and a flocculating agent.

The invention discloses a microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation. The method comprises steps of seed culture, liquid state fermentation and solid state fermentation. A Trichoderma strain is Trichoderma viride or Trichoderma harzianum. The invention not only provides a novel approach for harmlessness treatment and resource utilization of molasses alcohol wastes from sugarcane processing and a large amount of agricultural wastes generated by grape cultivation, besides, a solid Trichoderma agent obtained from the treatment can be applied to vineyards to increase biomass of Trichoderma for biological control in the environment, improve microecology and soil of the vineyard, inhibit pathogenic microorganisms for grapes in the microecology of the vineyard and reduce initiation factors for microbial diseases of grapes, thereby reducing application amount of pesticide, and enhancing fruit quality and security of the grapes.

The invention relates to the field of liquid milk, concretely, the invention relates to a colloid fluid suspension used for adding probiotics in acidic milk beverage and a method for producing probiotics-containing acidic milk beverage. According to the invention, the colloid fluid suspension used for adding aseptic probiotics in acidic milk beverage comprises the following components by weight, 1-5 parts of sugar and 0.1-0.6 parts of suspending agent; wherein the suspending agent comprises gellan gum, xanthan gum and acid resistant CMC according to a ratio of 1: 2 :3; 0.3-0.5 part of lactic acid and balance of water, wherein the pH value is 4.3-4.5. The method of the invention for producing probiotics-containing acidic milk beverage comprises the following steps: suspending aseptic embedded probiotics particles on the colloid fluid suspension, then ensuring asepsis and adding. According to the invention, probiotics stably survived in aseptic acidic milk beverage at normal temperaturecan be realized by the colloid fluid suspension, and probiotics is not easy to breed and grow in product shelf period, wherein the probiotics quantity in product is more than 107 cf umL, simultaneously the quality of the product can not be influenced, so that the active probiotics existed in the milk beverage product at normal temperature can be realized.

The invention discloses composite microbial granules for promoting growth of Pinus massoniana as well as preparation and application methods thereof. The composite microbial granules are prepared by enveloping solid culture of ectomycorrhizal fungi containing one or more of Suillus bovinus (Pers.) Roussel and Pisolithus tinctorius (Pers.) Coker and Couch as a mycorrhizal fungi, phosphate-solubilizing bacteria (Pseudomonas fulva)yL14 with preservation number of CCTCC M 2011356 and potassium bacteria with an enveloping material, adding nitrogenous fertilizer, phosphate fertilizer, potassium fertilizer, middle microelement and soil improvement agent, and mixing and granulating. By adopting the composite microbial granules disclosed by the invention, the absorption of nutrient elements by the Pinus massoniana root system is promoted, the utilization efficiency of the nutrient elements is improved, and the growth of Pinus massoniana is promoted; the water and fertilizer retention capabilityof the soil is enhanced, soil nutrients are supplemented, and microecology of the soil is improved; and the composite microbial granules have important significance in preventing and controlling landcapability decline of the soil, are convenient in material sources, low in cost and good in fertilizer efficiency, and are beneficial to popularization and application.

The invention discloses a feed additive capable of promoting health of livestock and poultry intestines and a preparation method. Montmorillonite modified with cetylpyridinium chloride is used for loading carvacrol. The montmorillonite is modified with the cetylpyridinium chloride, so that the layer spacing/ specific surface area and the hydrophobic character of the montmorillonite are improved, and the adsorption rate of the montmorillonite for the carvacrol is greatly increased. The montmorillonite has favorable biocompatibility and gastrointestinal tract mucosa substantivity, has the release control effect on the loaded carvacrol, can effectively exert the effects of resisting bacteria, resisting inflammation, resisting oxidation and the like, and has the synergistic effect and the synergism effect on antibacterial activity. The feed additive has the characteristics that the antibiotic action is high, and bacteria are not liable to cause drug resistance. The proliferation of harmfulbacteria in intestines of livestock and poultry can be effectively restrained, the height of intestinal villus and the intestinal barrier function are increased, secretion of digestive enzymes is facilitated, digestion of feed nutrients is facilitated, health of the intestines of the livestock and poultry is promoted, and the production performance of the livestock and poultry can be improved.

The invention provides a regulator for improving intestine health of juvenile fish of micropterus salmoides at a feed change stage and a preparation method and application of the regulator and belongsto the technical field of feed additives. The regulator comprises 5-30% of tea polyphenol, 5-20% of oxidized konjac glucomannan, 20-50% of linseed oil, 10-30% of inulin and 25-40% of a carrier. The preparation method specifically comprises the following steps: putting the linseed oil into a stainless steel reaction kettle, slowly adding the carrier, performing uniform stirring so as to obtain a linseed oil mixture, screening the linseed oil mixture by using an 80-100-mesh sieve, respectively adding the tea polyphenol, the oxidized konjac glucomannan and the inulin, performing uniform mixing and stirring, inspecting contents of components of the obtained mixture, and performing bottling and packaging. The obtained regulator is used in preparation of feeds for the juvenile fish of the micropterus salmoides at the feed change stage, intestine health of the juvenile fish of the micropterus salmoides at the feed change stage can be greatly improved, intestinal diseases can be reduced, anduse of antibiotics in the breeding process can be reduced.