Konjac oligomerization mannose sweet potato vinegar and preparation method thereof
A technology of oligomeric mannose and konjac, which is applied in the preparation of vinegar, biochemical equipment and methods, and methods based on microorganisms. short effect
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Embodiment 1
[0023] (1) Aspergillus niger enzyme solution: Potato dextrose agar medium (PDA medium) test tube solid activated Aspergillus niger, cultured at 32°C for 3 days; 250g of bran, 200g of water, moistened grits for 0.5h, sterilized at 121°C and 0.1MPa After 30 minutes, add 50g of water, mix evenly, and inoculate four pieces of the above-mentioned activated Aspergillus niger when cooled to 32°C, each with an area of 1cm 2 After culturing at 32°C for 36-48 hours, Aspergillus niger bran koji was obtained; with the same ingredient ratio, the Aspergillus niger bran koji for production was obtained by expanding the culture by ventilated koji making. Take 0.2 kg of Aspergillus niger bran koji, add 1.2 kg of water, stir for 5 minutes, and filter to obtain 1.0 kg of Aspergillus niger enzyme solution.
[0024] (2) Enzymatic solution of konjac oligomeric mannose: wash konjac, take 15kg, add 30kg of water, and grind to obtain konjac pulp. Konjac pulp, 0.5kg of enzyme solution, and 70kg of wa...
Embodiment 2
[0035] The steps of the method are the same as in Example 1, except that in "(3) saccharification mash", 150 kg of sweet potatoes and 110 kg of mannose oligosaccharide hydrolyzate are taken; in "(4) lactic acid and alcohol fermentation", 0.6% of Lactobacillus fermentum (MRS Medium liquid culture), Saccharomyces cerevisiae 0.3% and Aroma yeast 0.3%, the fermentation temperature is 19°C. For the vinegar prepared by the above operations, the total acid (calculated as acetic acid) is 4.24g / 100mL, the non-volatile acid is 1.1g / 100mL (calculated as lactic acid), and the mannose oligosaccharide is 3.1g / 100mL (calculated as mannose). The acetic acid taste is softer, the sweet potato flavor is more pronounced, and the aroma is stronger.
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