The invention provides a conductive graphene printing ink. The conductive graphene printing ink is composed of 0.001-80wt% of graphene, 1-60wt% of a linking material, 0.1-30wt% of an assistant, and the balance solvent. The invention also provides a preparation method of the conductive graphene printing ink. The conductive graphene printing ink has the advantages of good toughness, good die molding performance, good adhesion and good impact resistance; chemically doped graphene and chemically modified graphene in the graphene have good conductive, mechanical and thermal performances. The molecules of the chemically doped graphene comprise one or more of polyaniline, polyacetylene, polythiophene, polyparaphenylene and polypyrrole, and the functional groups of the chemically modified graphene comprise one or more of an anilino group, a pyrryl group, an imidazolyl group, a benzenesulfonic acid group, a thienyl group, a furyl group, a phenyl group, a hydroxy group, an ester group and derivative groups thereof, so the conductive performance, the mechanical performances and the dispersion stability of graphene in the printing ink are improved.