43results about How to "Has a promoting effect" patented technology

The invention discloses a method for promoting efficient water-soluble expression of arginine deiminase (ADI). The method comprises the following steps: connecting ADI gene derived from Pseudomonas putida with a pET-30a-c(+) carrier to construct a recombinant expression plasmid pET30a-ADI, and cotransforming the pET30a-ADI and pGro7 plasmid into E.coliBL21(DE3) to obtain double-plasmid-containing engineering bacteria; inoculating the engineering bacteria into an LB (Langmuir-Blodgett) culture medium containing 1-10 g / L D-glucose, adding L-arabinose, and culturing; and when the bacteria are cultured to a logarithmic phase, adding 1-10 g / L L-arginine and 0.1 mM IPTG (isopropyl-beta-D-thiogalactopyranoside), and carrying out induced culture at 16 DEG C under the condition of 180 rpm. The method disclosed by the invention can obviously enhance the water-soluble yield of the recombinant ADI protein, enhances the activity of the crude enzyme solution by 15 times, effectively overcomes the defect of difficulty in soluble expression of the recombinant ADI, provides technical support for application of industrialized large-scale production of citrulline and the like, and also provides a new idea for expression of other recombinant proteins.

The invention discloses a remediation agent suitable for heavy metal polluted soil in acidic farmland soil, which uses the crop seeds to be sown as the core, and the heavy metal polluted soil remediation agent as the seed coat, and the seed coat layer of the heavy metal polluted soil remediation agent includes crop waste material fermentation layer, clay mineral layer and water-absorbing polymer layer, wherein, the crop waste fermentation layer is in contact with the seed coat, and the repair agent coating layer is coated on the outside of the crop seeds in the form of layer-by-layer coating, and is in contact with the crop seeds when they are sown. At the same time, it is applied to the soil, which simplifies the application process of the restoration agent. The restoration agent exists around the roots of the crops to improve the utilization efficiency of the restoration agent; the wheat coated with the restoration agent is planted in the soil polluted by heavy metal cadmium, and the heavy metal in the harvested wheat The content of cadmium is lower than the limit standard of pollutants in food, the reduction rate of cadmium in wheat has reached more than 76%, and the passivation rate of available cadmium in soil has reached more than 92%.

The invention provides a surfactant composition applied to in vitro diagnostic reagents, which belongs to the technical field of surfactants, and the components include: sucrose-glucose-6-sodium carboxylate-fructose-6-sodium carboxylate-1-(N- lauryl formamide), sodium perfluorooctanoate, tetradecyltrimethylammonium bromide and Gemini surfactant 12‑2‑12, of which sucrose‑glucose‑6‑sodium carboxylate‑fructose‑6‑sodium carboxylate The content of ‑1‑(N‑dodecylformamide) is 5‑80mM, the content of sodium perfluorooctanoate is 10‑100mM, the content of tetradecyltrimethylammonium bromide is 10‑100mM, Gemini surfactant The content of 12‑2‑12 is 0.1‑2mM. The invention can optimize the reaction system of the in vitro diagnostic kit, improve performance indicators such as the repeatability, batch-to-batch difference, accuracy and stability of the kit, and help prolong the validity period of the in vitro diagnostic reagent.

A green recovery treatment device and recovery method for deactivated Raney nickel catalyst, including a vertical cylinder body and a liquid distribution kettle, an annular pipe is installed on the upper part of the cylinder body, the annular pipe is installed obliquely, and several connecting pipes are connected to the lower part of the annular pipe The connecting pipe is connected to the upper end of the cylinder, and the connecting pipe extends into the cylinder to cooperate with the inner wall of the cylinder. Several electromagnets are evenly installed on the outer circumference of the cylinder, and the liquid outlet of the liquid mixing kettle is connected to the upper inlet of the annular pipe. The bottom of the body is installed with a pump, the pump is connected to the liquid inlet of the liquid distribution kettle, the upper part of the body is equipped with a CO inlet with a valve, and the bottom of the body is equipped with a discharge pipe with a valve. In the present invention, in the recycling process of the deactivated Raney nickel catalyst, CO and acidic acetic acid which is good for the environment are used for treatment, so as to avoid the use of strong acid and have the advantages of environmental protection.

The invention discloses a coupled type waste water treatment device which comprises a biological aerated filter (7) and a catalytic ozonation tower (14) .The biological aerated filter (7) is in a downward flow mode, and sequentially comprises a first water inlet area (21), a first filler area (22) and a first water outlet area (23) from top to bottom .The catalytic ozonation tower (14) is a sealed tower, and sequentially comprises a second water inlet area (26), a second filler area (27) and a second water outlet area (28) from top to bottom .The sewage deep treatment technology is provided aiming at the urgent technical demand of petrochemical sewage plants in China for upgrading and reconstruction, phosphorus and organic matter can be removed at the same time, and the two units can be coupled.

The invention relates to a method for cooperative treatment of coking desulfurizing waste liquid, calcium-based solid waste and pollutants in industrial smoke. The method comprises the following steps: absorbing the oxidized industrial smoke by using absorbent slurry while controlling the pH of the slurry to 6 or higher during the absorption, wherein the absorbent slurry is a mixture of the calcium-based solid waste and the coking desulfurizing waste liquid; after the absorption, discharging the purified smoke, and performing solid-liquid separation on the absorbent slurry; performing impurityremoval and pH regulation on the obtained liquid phase, and heating for decomposition; performing solid-liquid separation to obtain an ammonium thiocyanate product. The method provided by the invention can realize efficient removal of sulfur dioxide, nitrogen oxide and mercury in smoke while recycling an ammonium thiocyanate product with high added value. The method provided by the invention canbe used for performing cooperative treatment on the waste gas, wastewater and waste residue generated in an industrial production process, has the advantages of simple process, low raw material cost and no wastewater discharge in the whole process, realizes cyclic economy of dealing with waste by waste, is suitable for industrial application and creates good economic benefits.

The invention discloses a scorpion venom heat-resistant synthetic peptide and its application, belonging to the field of polypeptide drug research and development. The polypeptide of the present invention is the amino acid sequence of the scorpion venom heat-resistant peptide (SVHRP) detected from the venom of the traditional Chinese medicine East Asian scorpion scorpion (BmK). A sample of the heat-resistant peptide extract from scorpion venom with pharmacodynamic activity of Alzheimer's disease (premature Alzheimer's disease) was separated by LaGM composite material and repeated rapid magnetic separation, and then performed parallel mass spectrometry using nanoliter reversed-phase chromatography-electrospray mass spectrometry (nanoLC-ESI-MS). A polypeptide sequence consisting of 15 amino acid residues was detected in the experiment; a heat-resistant synthetic peptide of scorpion venom was prepared through solid-phase chemical synthesis, chromatographic purification and mass spectrometry identification. Its amino acid sequence is shown in SEQIDNo 1, which maintains the The pharmacodynamic activity and safety of the scorpion venom heat-resistant peptide, and the bioactivity of the scorpion venom heat-resistant peptide also promotes the reverse differentiation of glial cells into neural stem cells.