Stable rooting method for poplar cuttage
A technology of cuttings and poplars, applied in the field of tree species propagation, can solve the problems of low survival rate of cuttings, increased costs, waste of cuttings, etc., and achieve the effects of shortening the cycle, saving costs, and reducing the concentration of use
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Embodiment 1
[0036] A kind of poplar cutting stable rooting method:
[0037] (1) Preparation of explants: cut strong branches from the adult black poplar with good growth, cut the branches with a length of about 12 cm, and have 4 bud points and 2 leaves as explants.
[0038] (2) The epidermis at about 1 cm above the base of the explant is stripped off by circumcision, the stripping width is about 0.5 cm, and the stripping thickness is the sum of the thickness of the bark and phloem of the explant (in order not to damage the xylem Standard) to obtain explants with circumcision at the base, and then wrap absorbent cotton on the top of the explants and fix them with rubber bands.
[0039] (3) Put the explants obtained in step (2) into a plastic tissue culture bottle and add clear water, the amount of water should be higher than the girdling of the cuttings, and then put the explants at a temperature of 26±1°C , the light time is 16h, and the rooting culture is carried out in a greenhouse wit...
Embodiment 2
[0042] Same as Example 1, the difference is that the concentration of indolebutyric acid in step (3) is 0.1 mg / mL.
Embodiment 3
[0044] Same as Example 1, the difference is that the concentration of indolebutyric acid in step (3) is 0.5mg / mL.
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