The invention relates to a
screening method of bacterial strains and specially relates to a
screening method of Vc two-step
fermentation bacterial strains. Transformed
bacteria and
associated bacteria in Vc two-step
fermentation are cultured in seed solution of the Vc two-step
fermentation in a
mixed mode.
Fermentation liquor is centrifuged, and then supernatant liquor is transformed to be
bacteria liquor A. In addition, the associated liquor in the Vc two-step fermentation is inoculated in culture medium and cultured for 20-28 hours. After culturing, a sterile filtering membrane filters filtering liquor as fermentation liquor B, the transformed
bacteria liquorA and the fermentation liquor B are mixed and then diluted. Diluted liquor is extracted to be coated on a isolation culture medium, and cultured at the temperature of 29-37 DEG C for 4-8 days.
Bacteria colony of the transformed bacteria is formed. Sterile
sodium bromothymol blue indicating liquor with 0.1% concentration is added to the isolation culture medium where the bacteria colony of the transformed bacteria is formed and still for hours from 0.5 to 2.
Transformation efficiency is judged as being high or low according to yellow circle around the bacteria colony, so that transformed bacteria strains with high-efficiency transformation capacity are quickly screened. According to the
screening method, the condition that an existence of the
associated bacteria has an effect on transformation function is avoided, and besides the function that the associated liquor actives growth of the transformed bacteria and
acid production is guaranteed.
Color changes of indicator can also accurately indicate the transforming capacity of the transformed bacteria, and screening efficiency is greatly improved.