The invention discloses a
chemiluminescent immunoassay kit for quantitatively detecting
digoxin. The
chemiluminescent immunoassay kit is characterized in that the
chemiluminescent immunoassay kit includes a
reagent R1, a
reagent R2 and a
reagent R3, wherein the reagent R1 is a suspension containing
streptavidin magnetic particles, the reagent R2 is a solution containing a
digoxin monoclonal antibody marked by a chemiluminescent marker, and the reagent R3 is a solution containing
digoxin derivatives marked by
coupling markers. According to the chemiluminescent
immunoassay kit provided by the invention, the digoxin is a small molecular substance, thus detection is conducted through the principle of a competitive method, the time and cost are saved, and the kit has the high specificity of animmune reaction, further has the high sensitivity of a luminous reaction and has the advantages of high sensitivity, high specificity, wide
linear range, good reagent stability, easy and convenient operation and easy
automation; a preparation method provided by the invention is simple in technology, and the kit is long in validity period and relatively low in cost;
rapid detection is convenient torealize, the requirements for instruments are simple, and fully-automatic operation is convenient to realize.