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48results about How to "Fermentation conditions are easy to control" patented technology
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Method for producing DHA by Schizochytrium in high-density culture through fermentation
ActiveCN101812484ANo dependencyGuaranteed qualityMicroorganism based processesFermentationBiotechnologyProduction rate
The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.
Owner:XIAMEN HUISON BIOTECH
Biocontrol microorganism capable of generating plant pathogenic fungi-resistant lipopeptide, and applications of pesticide preparation of biocontrol microorganism
The invention relates to a biocontrol microorganism capable of separating from sea mud products and generating plant pathogenic fungi-resistant lipopeptide, and applications of the biocontrol microorganism. The microorganism is a Bacillus licheniformis 9912 strain (in China Center For Type Culture Collection, with collection number of CCTCC No. M2010343). The microorganism is fermented to generate cyclic lipopeptide compounds 6-Abu C16fengycin, anteiso-C15surfactin and anteiso-C15surfactin methyl ester which have wide-spectrum plant pathogenic fungi prevention bioactivity as main metabolite; the microorganism and the fermented metabolite can conventionally adsorb a carrier and auxiliaries to prepare a microbial preparation and a biopesticide preparation capable of effectively preventing and treating cucumber and botrytis cinerea, blight, tomato stem rot, apple tree canker, valsa mali miyabe et yamada, pear tree blight and the like, and have the characteristics of being low in cost, efficient in biocontrol, non-residual, and safe to human and livestock.
Owner:SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
Aspergillus niger and applications thereof in preparation of Pu'er tea theine compounds
ActiveCN106978350AShorten the production cycleRealize large-scale industrializationFungiEnergy inputMicroorganismMicrobial transformation
The invention relates to aspergillus niger M03 and applications of the aspergillus niger in preparation of Pu'er tea theine through microbial conversion. The aspergillus niger M03 strain capable of realizing bioconversion for preparing Pu'er tea theine is separated from Pu'er ripe tea, is preserved in the China General Microbiological Culture Collection Center (CGMCC), is assigned with the accession number of CGMCC No.10930. According to the aspergillus niger M03 and the applications, the aspergillus niger M03 strain is taken as a fermenting strain, the piling solid fermentation is adopted, and the sun-withering crude tea leaves of Yunnan large-leaf tea are adopted as the raw material; or liquid fermentation is adopted, catechin compounds and theanine are adopted as substrates, piling solid fermentation and liquid fermatneion are carried out, and thus the Pu'er tea theine compounds are prepared. The aspergillus niger M03 can be used for specifically preparing the Pu'er tea theine compounds, has the advantages that the environment is protected, the operation is simple, and the popularization is convenient, and thus the application prospect is very good.
Owner:PEKING UNIV
Method for producing DHA by Schizochytrium in high-density culture through fermentation
ActiveCN101812484BNo dependencyGuaranteed qualityMicroorganism based processesFermentationBiotechnologyProduction rate
The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.
Owner:XIAMEN HUISON BIOTECH
Fermentation and Brewing Technology of Xiaoqu Fen-flavor Liquor in Small Dressing Ceramic Jar and Small Dressing Ceramic Jar
InactiveCN102286335AReduce dosageReduce or avoid infectionAlcoholic beverage preparationFlavorMetallurgy
The invention relates to a brewing process of xiaoqu elegant white wine by using small-capacity ceramic jar fermentation. The brewing process comprises selecting grains, washing the grains, soaking, cooking, spreading and airing, mixing with yeast, saccharifying, fermenting and distilling steps; and the brewing process is characterized by adding yeast-mixed and saccharified fermented grains to a ceramic jar, pottery jar or porcelain jar, uniformly compacting, adding water the amount of which is 90-120% the weight of the fermented grains, sealing and fermenting, adjusting the ambient temperature to guarantee that the internal temperature of the fermentation jar reaches 38-40 DEG C in winter and spring and 33-36 DEG C in summer and autumn within 72 hours, maintaining the ambient temperatureat 24-26 DEG C after the fermented grains is added to the jar for 90-120 hours, and after the fermentation is carried out for 6-8 days or the residual sugar in the fermented grains is approximate to zero, pouring the fermented grains into a retort pot for distilling. In the brewing process provided by the invention, a small-capacity ceramic jar is used as a fermentation container, thereby facilitating cleaning and disinfection, improving wine quality and taste and improving wine yield; and the white wine brewed by the brewing process provided by the invention has elegant and pure flavor as well as mellow, sweet and refreshing taste, and is gentle, natural, harmonious and joyful.
Owner:河南东坡酒业集团有限公司
Preparation and applications of Paenibacillus polymyxa GY40 and wettable powder of Paenibacillus polymyxa GY40
ActiveCN108277172AStrong salt toleranceLow growth temperatureBiocideBacteriaPaenibacillus polymyxaBacterial strain
The invention discloses a Paenibacillus polymyxa GY40 bacterial strain. The preservation number is CGMCC NO.14481. The invention also discloses a spore production fermentation culture method of the Paenibacillus polymyxa GY40 bacterial strain, and a method used for preparing bacterial powder and a wettable powder from the Paenibacillus polymyxa GY40 bacterial strain. Compared with terrestrial microorganisms, the Paenibacillus polymyxa GY40 bacterial strain possesses following advantages: salt resistance is excellent, growth temperature is low, and antibacterial effect is excellent. The spore production fermentation culture method is simple in formula; the main raw materials are agricultural product leftovers; the cost is low; the raw materials are widely available; bacterial strain growthspeed is high; fermentation temperature is low; fermentation time is short; fermentation conditions are convenient to control; fermentation spore yield is high; raw material cost is reduced; fermentation energy consumption is reduced; fermentation production efficiency is increased; the wettable powder possesses excellent preventing and treating effect on wheat scab and fusarium wilt of cucumber,and can be used in plantation of wheat and cucumber.
Owner:JIANGSU GENGYUN CHEM CO LTD
Solid-state fermentation device and system as well as fermentation method
ActiveCN105062883AAchieving standardized workClear working structureBioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyBiochemical engineering
The invention discloses a solid-state fermentation device and system as well as a fermentation method. The fermentation device is divided into three parts with clear functions, namely, a working bin, a fermentation bin and a quantitative discharging bin, and the standard fermentation system is constructed. The quantitative discharging bin is controlled to discharge materials quantitatively every day, the working bin distributes the materials into the fermentation bin, quantitative material distribution is controlled, and the materials in the fermentation bin are fermented in a layered manner. Further, turnover boxes are adopted for discharging and are storage and transporters for fermented products. The components of the device are well combined, equipment maintenance and fermentation production as well as standard operation of the fermentation technology are guaranteed due to adoption of standard equipment, the automation degree is high, multiple groups of equipment can be combined for work while operators are not increased excessively, the management cost and the production cost are lower, mass production and mass operation are facilitated, and operation and control are easy, so that the fermented products as well as storage and transportation of the products are also standardized.
Owner:麦行
Solid-state fermentation device with uniform material distribution function and fermentation method
ActiveCN105062875ARealize standardized operationEasy to combineBioreactor/fermenter combinationsBiological substance pretreatmentsBatch operationProcess engineering
The invention discloses a solid-state fermentation device with a uniform material distribution function and a fermentation method. The solid-state fermentation device is divided into a working bin, a fermentation bin and a quantitative discharging bin with clear functions, and a standard fermentation system is constructed. Everyday quantitative discharging of the quantitative discharging bin is controlled, a working frame is arranged in the working bin, stirring devices and material distribution devices are uniformly arranged on the working frame, quantitative and uniform material distribution in the fermentation bin is realized, and materials in the fermentation bin are subjected to layered fermentation. The combination property of the parts is good, equipment maintenance, fermentation production and standard operation of a fermentation process are guaranteed with adoption of standard equipment, the automation degree is high, multiple groups of equipment can be combined for working without excessive increase of operators, the management expense and production cost are lower, batch production and batch operation are facilitated, and operation and control are easy, so that standardization of fermented products as well as storage and transport operation of the products is guaranteed.
Owner:麦行
Low temperature protease production strain, low temperature protease produced by the same and gene of enzyme
InactiveCN104818225AIncrease productionPollution abilityBacteriaHydrolasesAlkaline proteaseOptimum growth
The invention relates to a low temperature protease production strain, low temperature protease produced by the same and a gene of the enzyme. A strain of planococcussp. producing low temperature protease is separated from the southern Indian Ocean deep-sea sediment for the first time, the preservation number of the strain is CGMCC No.8088, the optimum growth temperature and the optimum enzyme production temperature are both 20DEG C, and the fermentation level protease yield can reach 280U / mL. The optimum operation temperature of the protease produced by the strain is 30-37DEG C, the optimum pH is 7.5-9.0, under the condition of 30DEG C, over 80% of the highest catalytic activity can be maintained, and in the range of 20-25DEG C, the protease still has high catalytic activity. The protease produced by the strain belongs to typical low temperature alkaline protease and has good catalytic activity at normal temperature, thus having high research value in food processing industry, cold water detergent development and other aspects.
Owner:TIANJIN UNIV OF SCI & TECH
Bacillus subtilis for high-yield gamma-polyglutamic acid and fermentation method of bacillus subtilis
InactiveCN109943495AIncrease productionNarrow molecular weight distributionBacteriaMicroorganism based processesMicroorganismMicrobiology
The invention discloses bacillus subtilis for high-yield gamma-polyglutamic acid and a fermentation method of the bacillus subtilis. The preservation number of the bacillus subtilis is CGMCC NO: 9383,and the bacillus subtilis is preserved in China General Microbiological Culture Collection Center on June 25th, 2014. The gamma-polyglutamic acid is fermented and extracted through the four steps ofstrain activation, seed culture, tank loading fermentation and extraction of the gamma-polyglutamic acid, the fermentation method of the bnacillus subtili is simple, the production cost is low, the fermentation condition is easy to control, the purity of the fermentation product is high, the yield of the gamma-polyglutamic acid is as high as 56.5 g / L, and the bacillus subtilis can be convenientlyapplied to the production of the gamma-polyglutamic acid.
Owner:ZHEJIANG HUIJIA BIOTECH
Solid state fermentation device, system and fermentation method of movable material distribution
ActiveCN105062876ARealize standardized operationEasy to combineBioreactor/fermenter combinationsBiological substance pretreatmentsBatch operationProcess engineering
The invention discloses a solid state fermentation device, system and fermentation method of movable material distribution. The fermentation device comprises functionally clear three parts, namely, a working bin, a fermentation bin and a quantitative discharge bin which establish the standardized fermentation system, wherein quantitative discharge is carried out every day through controlling the quantitative discharge bin; a movable rack is arranged in the working bin; a stirring device and a material distributing device are arranged on the movable rack; quantitative and uniform material distribution to the fermentation bin and layered fermentation of materials in the fermentation bin are achieved through the operation of the movable rack. According to the solid state fermentation device, system and fermentation method of movable material distribution, all the parts are good in modularity, and standardized operation of equipment maintenance, fermentation production and fermentation technology is guaranteed through the adoption of the standardized equipment; the fermentation device is high in degree of automation, multiple groups of equipment can be combined to work without adding too many operating staff, and the administration expense and production cost are relatively low, so that batch production and batch operation are facilitated, the device is easy to operate and control, and standardization of the fermented product, storage and transportation of the product is guaranteed.
Owner:麦行
Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof
ActiveCN108148778AWide range of antibacterialGood stabilityBiocideBacteriaFertilizerBacillus amyloliquefaciens
The invention discloses an ocean-originated bacillus amyloliquefaciens GY30 strain. The preservation number of the ocean-originated bacillus amyloliquefaciens GY30 strain is CGMCC NO. 14480. The invention also discloses a spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain and a method for preparing bacterium power through the bacillus amyloliquefaciens GY30 strain. The bacillus amyloliquefaciens GY30 strain has the advantages of being high in salt tolerance, low in growth temperature, good in antibacterial effects, wide in antibacterial range and easy to culture, which do not exist in terrigenous microorganisms. The spore production fermentation medium composition and the culture method of the bacillus amyloliquefaciens GY30strain are simple, applied raw materials are mainly agricultural product leftovers, thereby being low in price, extensive in resources, high in strain growth speed, low in fermentation temperature, short in fermentation time, easy to control in fermentation conditions, high in spore fermentation yield and capable of reducing raw material costs and fermentation energy consumption and improving thefermentation production efficiency; bacterium powder prepared through the spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain is high ineffects on processing microorganism fertilizers or rice sheath blight disease preventing pesticides.
Owner:JIANGSU GENGYUN CHEM CO LTD
Method for preparing plectasins by saccharomyces cerevisiae
ActiveCN104232712AClear genetic backgroundHigh purityMicroorganism based processesFermentationBiotechnologyAntimicrobial peptides
The invention discloses a method for preparing plectasins by saccharomyces cerevisiae. The method comprises the following steps: a, transforming and amplifying saccharomyces cerevisiae genes; b, building recombinant plasmids pYES2-CPS-Plec of the saccharomyces cerevisiae genes in yeast cells; c, transforming INVSC1 cells of saccharomyces cerevisiae genes by the recombinant plasmids pYES2-CPS-Plec; d, screening positive bacteria strains, and performing digestion and identification; e, cultivating transformed bacteria to obtain antimicrobial peptide saccharomyces cerevisiae genes; and f, identifying the activity of the antimicrobial peptide saccharomyces cerevisiae genes. The plectasins provided by the invention has an obvious bacteriostatic action on a saccharomyces cerevisiae expression system, and the antibacterial effect is stronger than that of the antibacterial effect before modification. The saccharomyces cerevisiae expression system provided by the invention has the following advantages of being better in antibacterial effect, fast in growth rate and high in yield, is likely to realize high-density culture and does not need adopt methanol induction; furthermore, a protein product for expressing secretion is likely to be separated and purified; moreover, the saccharomyces cerevisiae expression system has a plurality of posttranslational modification features, such as polypeptide folding, glycosylation, methylation, acetylation and the like; in addition, due to a clear genetic background, the saccharomyces cerevisiae expression system is likely to operate an expression vector.
Owner:CHANGSHA ZHONGKEJINGBO BIOTECH
A high-fiber feed biodegradation fermentation device
The invention provides a high fiber forage biodegradation fermenting system suitable for multi-bacterial composite fermentation, and solves the key problems in production that charging / discharging in a fermentation process is large in labor intensity, fermentation period is long, output is low, fermentation effect is poor and fermentation conditions are hard to control. The high fiber forage biodegradation fermenting system provides a novel fermentation chamber and matching facility, wherein a semi-automatic fermentation vehicle is designed in the fermentation chamber, and the vehicle is good in air permeability, convenient for loading and unloading, more in bearing material, and convenient in motion and discharging; the fermentation chamber is excellent in sealing, can realize automatic temperature control, automatic moisture control, and automatic disinfection; the system is large in single fermentation capacity, convenient in operation, short in fermentation period, and saves energy; the fermentation system saves time and labor, saves energy, and fermentation conditions can be easily controlled, thus better satisfying factory forage fermentation production needs.
Owner:QINGDAO AGRI UNIV
High fiber forage biodegradation fermenting device
The invention provides a high fiber forage biodegradation fermenting system suitable for multi-bacterial composite fermentation, and solves the key problems in production that charging / discharging in a fermentation process is large in labor intensity, fermentation period is long, output is low, fermentation effect is poor and fermentation conditions are hard to control. The high fiber forage biodegradation fermenting system provides a novel fermentation chamber and matching facility, wherein a semi-automatic fermentation vehicle is designed in the fermentation chamber, and the vehicle is good in air permeability, convenient for loading and unloading, more in bearing material, and convenient in motion and discharging; the fermentation chamber is excellent in sealing, can realize automatic temperature control, automatic moisture control, and automatic disinfection; the system is large in single fermentation capacity, convenient in operation, short in fermentation period, and saves energy; the fermentation system saves time and labor, saves energy, and fermentation conditions can be easily controlled, thus better satisfying factory forage fermentation production needs.
Owner:QINGDAO AGRI UNIV
High-yield culture medium using dickeyazeae to produce antibiotic zeamine and culture method
ActiveCN103757067AIncrease productionEasy to prepareBacteriaMicroorganism based processesBiotechnologySucrose
The invention discloses a high-yield culture medium using dickeyazeae to produce broad-spectrum antibiotic zeamine and a culture method. 1L of the culture medium contains 1-30g of a carbon source, 0.5-5g of a nitrogen source, 0.05-5g of an inorganic salt of and 0.1-5g of an amino acid. The carbon source is one or a plurality of glucose, sucrose, mannitol, glycerin or fructose; the nitrogen source is one kind or a plurality of ammonium sulfate, sodium nitrate, ammonium nitrate or ammonium chloride; the inorganic salt is one or a plurality of ferrous sulfate, calcium chloride, manganese chloride, magnesium sulfate or potassium chloride; the amino acid is one or a plurality of aspartic acid, asparagine or proline. The yield of the antibiotic produced by use of the high-yield culture medium is 20-30 times more than the antibiotic produced by use of a basic culture medium, the high-yield culture medium is simple in components, simple in preparation, low in cost, and easy in purification and separation of the antibiotic, and the culture method has the advantages of simple operation and easy control of culture conditions, and is beneficial for large-scale preparation of the antibiotic.
Owner:SOUTH CHINA AGRI UNIV +1
Production method of glucose oxidase for feeding
InactiveCN104381618AFermentation conditions are easy to controlNot easy to contaminate bacteriaAnimal feeding stuffPenicillium bilaiaeBiotechnology
The invention relates to a production method of glucose oxidase for feeding, and aims at solving the problem of an existing common solid state fermentation technology that the fermentation conditions are difficult to control. The production method of the glucose oxidase for feeding comprises the following steps: culturing activated penicillium notatum for at least one time to obtain penicillium notatum seeds; fermenting the penicillium notatum seeds by adopting a fluidized bed solid state fermentation method; after the fermentation, drying a solid culture medium, a fermentation product and a fermentation strain in a fluidized bed solid state fermentation tank; and then crushing the product to obtain a glucose oxidase finished product. The production method is easy to control, the product is not easily polluted by infectious microbes, and three wastes are not generated so that no pollution to the environment is caused; and after the fermentation, the fermentation product, namely the glucose oxidase, does not need to be purified, and rice dregs and / or rice bran, penicillium notatum strains and the product glucose oxidase can be directly dried to prepare the glucose oxidase for feeding. The production method has a simple process, and the biological cycle utilization of the rice dregs and / or rice bran can be promoted.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Method for preparing oligosaccharides with side chains by utilizing thermophilic filamentous fungus fermentation one-step method
InactiveCN108048506ALow costReduce the cost of separation and purificationMicroorganism based processesFermentationThermomyces lanuginosusSide chain
The invention discloses a method for preparing oligosaccharides with side chains by utilizing a thermophilic filamentous fungus fermentation one-step method. The method is characterized in that the oligosaccharides with the side chains are prepared by utilizing a method for solid fermentation or liquid fermentation of thermomyces lanuginosus. An experiment verifies that through the thermophilic properties of the thermomyces lanuginosus, fermentation conditions are easy to control in the production process of the thermomyces lanuginosus; meanwhile, the thermomyces lanuginosus can utilize cheapcorn cobs, bran and other agricultural and sideline products to ferment and produce the oligosaccharides with the arabinose side chains; the invention disclosed by the invention is a sustainable production method, can reduce the raw material cost and the separation and purification cost and is an environment-friendly and efficient production process.
Owner:SHANDONG UNIV
Ester-flavor mould cheese and preparation method thereof
ActiveCN114097890ARich ester aromaDelicate tasteCheese manufactureBiotechnologyLactic acid bacterium
The invention relates to the technical field of preparation of cheese products, and particularly discloses ester-flavor mould cheese and a preparation method thereof. The ester-flavor mould cheese is prepared from the following raw materials in parts by weight: 100 to 120 parts of dairy products, 1 to 5 parts of white granulated sugar, 0.5 to 2.5 parts of lactic acid bacteria leavening agents, 0.02 to 0.1 part of saturated CaCl2 aqueous solution, 0.3 to 1.5 parts of saccharomycetes, 0.1 to 0.9 part of geotrichum candidum and 0.05 to 0.45 part of chymosin. According to the invention, anaerobic fermentation and aerobic fermentation are combined by adding saccharomycetes, so that the cheese has unique ester flavor and a special appearance structure; and by internally inoculating geotrichum candidum, the original processing mode of the mould cheese is improved, and the mould cheese is endowed with unique flavor and structural characteristics.
Owner:SHENYANG AGRI UNIV
Preparation method and preparation device of polylysine
InactiveCN111534559ACausing leakage problemsEasy to adjust controlBioreactor/fermenter combinationsFungiProcess engineeringAnti bacterial
The invention belongs to the field of polylysine products, and particularly relates to a preparation method and a preparation device of polylysine. The preparation method of polylysine comprises the steps of seed selection, enlarged culture, fermentation preparation and filtration clarification. A polylysine product produced by the preparation method of polylysine is stable in passage and high inpolylysine production capacity; in addition, related experiments find that the polylysine product produced by the preparation method of polylysine is high in antibacterial ability, good in fresh-keeping effect and relatively high in safety; more importantly, compared with a traditional biological fermentation device, the preparation device for producing the polylysine product can easily control and adjust all related physical parameters in the fermentation condition, and a stirring assembly is arranged according to the electromagnetic induction driving principle, so that the problems that a rotating shaft is mounted on the sealing cover, the sealing cover is inconvenient to open, and the preparation device is easy to leak in the fermentation process due to the fact that the bottom end of the preparation device penetrates through the rotating shaft can be avoided.
Owner:樟树市狮王生物科技有限公司
Method for producing biological antibacterial feed with garlic leaf as additive
The invention provides a method for producing a biological antibacterial feed with a garlic leaf as an additive. A protein feed for a rabbit is prepared by crushing corncob and sweet potato dregs into powder, adding a certain amount of a nitrogen source, inorganic salt and a solid accessory material, inoculating a certain amount of bacterial strains in proportion, carrying out fermentation at 55 to 65 DEG C under sealing conditions for 15 to 20 d, carrying out drying in the sun and adding 10.0 to 15.0% of garlic leaf powder. The bacterial strains comprise bacillus subtilis, rhizopus oryzae, Geobacillus stearothermophilus, pyrolytic cellulolytic bacteria and Thermoactinomyces, and an inoculation volume ratio is 1: 1: 1: 2: 2: 3, preferably. The fermented feed produced by using the method has high crude protein content, low crude fiber content, a great number of beneficial components and good palatability, can improve feed intake of commercial rabbits, enhance immunity, reduce incidence of disease and improve feeding quality, has a reasonably designed formula, is simple and convenient to prepare, has low cost and high benefits and is applicable to large-scale pollution-free rabbit culturing by vast peasant households and in culturing farms.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
Immobilized cell single-tank high-strength continuous fermentation process for succinic acid
InactiveCN101153294BNutrition easyReduce fermentation costsMicroorganism based processesFermentationCurrent velocityContinuous fermentation
The invention discloses a single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid, which comprises the procedures including that: firstly, seed liquid of high-activity actinobacillus capable of producing succinic acid is prepared; secondly, the seeds are proliferated in cultivation with a10L pot; thirdly, the 10L-pot solified cells are prepared; fourthly, the repeated single-pot fermentation with the 10L pot is carried out, then the seeds are inoculated into a fresh substrate, the first batch of fermentation is controlled within 24 hours, followed by 9 batches of continuous fermentation, each of which lasts for 16 hours, then the amphoteric anaerobic cultivation pattern is controlled through the current velocity of the combined air of CO2 and H2, the pH of the fermentation liquid is controlled with fed-batch of calcium oxide, wherein, at the end of each batch of fermentation, the next batch of fermentation is carried out through intercepting the solidified cells with positive-pressure sterilized operation with a propeller filter arranged inside the pot, the fermentation intensity is stabilized above 3.10g / (h.L), and the single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid is completely accomplished.
Owner:HEFEI UNIV OF TECH
Brewing process of xiaoqu elegant white wine by using small-capacity ceramic jar fermentation and small-capacity ceramic jar
InactiveCN102286335BReduce dosageReduce or avoid infectionAlcoholic beverage preparationYield (wine)Chaptalization
The invention relates to a brewing process of xiaoqu elegant white wine by using small-capacity ceramic jar fermentation. The brewing process comprises selecting grains, washing the grains, soaking, cooking, spreading and airing, mixing with yeast, saccharifying, fermenting and distilling steps; and the brewing process is characterized by adding yeast-mixed and saccharified fermented grains to a ceramic jar, pottery jar or porcelain jar, uniformly compacting, adding water the amount of which is 90-120% the weight of the fermented grains, sealing and fermenting, adjusting the ambient temperature to guarantee that the internal temperature of the fermentation jar reaches 38-40 DEG C in winter and spring and 33-36 DEG C in summer and autumn within 72 hours, maintaining the ambient temperatureat 24-26 DEG C after the fermented grains is added to the jar for 90-120 hours, and after the fermentation is carried out for 6-8 days or the residual sugar in the fermented grains is approximate to zero, pouring the fermented grains into a retort pot for distilling. In the brewing process provided by the invention, a small-capacity ceramic jar is used as a fermentation container, thereby facilitating cleaning and disinfection, improving wine quality and taste and improving wine yield; and the white wine brewed by the brewing process provided by the invention has elegant and pure flavor as well as mellow, sweet and refreshing taste, and is gentle, natural, harmonious and joyful.
Owner:河南东坡酒业集团有限公司
Multi-stage liquid submerged fermentation method of Ganoderma lucidum
The invention discloses a multi-stage liquid submerged fermentation method of Ganoderma lucidum. The method comprises the following steps: (1) a first fermentation stage (0-26 h), wherein fermentationhas a pH of the original pH of a culture medium, a temperature of 26 DEG C, and a stirring speed of 80 r / min; (2) a second fermentation stage (26-36 h), controlling the pH to 4.0, temperature to 26 DEG C, and stirring speed at 80 r / min for fermentation; (3) a third fermentation stage (36-81 h), controlling the pH to 4.5, temperature to 26 DEG C, and stirring speed at 80 r / min for fermentation; and (4) a fourth fermentation stage (81-168 h), controlling the pH to 5.0, temperature to 26 DEG C, and stirring speed at 80 r / min for fermentation, and ending the fermentation at 168 h. The Ganoderma lucidum mycelium produced by the method has a significantly increased content of ganoderma triterpenes in mycelium.
Owner:SHANGHAI ACAD OF AGRI SCI
Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive
InactiveCN102326673BReduce energy consumptionFermentation conditions are easy to controlBacteriaClimate change adaptationFood additiveCulture fluid
Owner:SHANXI WEIMINSHENG TECH
A kind of lysine bacillus zcgt05 and its application
ActiveCN113151060BEasy to operateFermentation conditions are easy to controlBacteriaHydrolasesBiotechnologyMicrobiology
The invention relates to the field of biotechnology, in particular to a lysine bacillus ZCGT05 and its application. The present invention provides a bacterial strain with a preservation number of CGMCC NO.16935, and discloses the application of the bacterial strain to produce chitin deacetylase. The present invention also provides the screening and cultivation of the bacterial strain and the preparation of chitin deacetylase In the method, the bacterial strain is fermented and centrifuged, and the supernatant can be a chitin deacetylase solution. And after several experiments, the optimal fermentation condition for the strain to produce chitin deacetylase is provided.
Owner:JIANGSU OCEAN UNIV
Preparation and application of Paenibacillus polymyxa gy40 and its wettable powder
ActiveCN108277172BStrong salt toleranceLow growth temperatureBiocideBacteriaBiotechnologyMicroorganism
The invention discloses a Paenibacillus polymyxa GY40 strain, and its preservation number is: CGMCC NO.14481. The invention also discloses a spore-forming fermentation culture method of the Paenibacillus polymyxa GY40 strain and a method for preparing bacteria powder and wettable powder by using the Paenibacillus polymyxa GY40 strain. The bacterial strain of the present invention has the advantages of strong salt tolerance, low growth temperature, and strong antibacterial effect that terrestrial microorganisms do not have; its spore-forming fermentation method has a simple formula, and the raw materials are mainly agricultural product leftovers, with low price, wide sources, and rapid growth of the bacterial strain. Fast, low fermentation temperature, short fermentation time, easy control of fermentation conditions, high yield of fermentation spores, reduced cost of raw materials, reduced energy consumption of fermentation, improved fermentation production efficiency, the wettable powder prepared by this strain has good control Wheat head blight and cucumber wilt can be used for wheat and cucumber planting.
Owner:JIANGSU GENGYUN CHEM CO LTD
A low-temperature protease-producing Exiguobacterium siberia and its application
ActiveCN111893067BFermentation conditions are easy to controlHigh catalytic activityBacteriaHydrolasesBiotechnologyExiguobacterium sibiricum
The invention discloses a low-temperature protease-producing Microbacterium siberia ( Exiguobacterium sibiricum ), preserved in the China General Microorganism Culture Collection and Management Center with the preservation number of CGMCC No.20358. The low-temperature protease-producing Exiguobacterium siberia was screened from Antarctic krill, and its optimum growth temperature and optimum enzyme-producing temperature are both close to the temperature in the natural environment, so the heating or cooling process can be omitted during the fermentation process. The optimal reaction temperature of the protease produced by this strain is about 37°C, and it has good catalytic activity (more than 60% of the highest catalytic enzyme activity) between 0-60°C, with low temperature adaptability and good thermal stability. sex.
Owner:QUFU NORMAL UNIV
A strain of Rhodotorula biobversus producing carotenoids and its application
ActiveCN111808763BHigh yieldImprove securityFungiMicroorganism based processesBiotechnologyMicroorganism
The present invention discloses a carotenoid-producing Rhodotorula biobovata and its application, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, with the preservation number CGMCC No.20126 and the preservation date being June 22, 2020 . Rhodotorula biobversus ZQ38 provided by the present invention belongs to the source of marine microorganisms and is an excellent strain for producing natural carotenoids. The conditions are easy to control, the nutritional requirement is low and the yield of carotenoids is high, and the strain has broad application prospects in the biosynthesis of carotenoids.
Owner:LUDONG UNIVERSITY
Fermentation extraction method of ganoderan, ganoderan composition and application of ganoderan composition
PendingCN114672527AImprove moisturizing and skin rejuvenationFermentation conditions are easy to controlCosmetic preparationsToilet preparationsMolecular biologyCellulase
Owner:CHINA PHARM UNIV
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