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Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive

A technology for feed additives and photosynthetic bacteria is applied in the field of producing aerobic fermentation photosynthetic bacteria for livestock and poultry breeding feed additives.

Inactive Publication Date: 2013-02-13
SHANXI WEIMINSHENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This photosynthetic bacteria preparation is a liquid preparation produced by photosynthetic bacteria, but because the whole process of its production and fermentation is always under anaerobic light, the production efficiency is low and the production energy consumption is high

Method used

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  • Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive
  • Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive
  • Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Add 2.0 grams of sodium malate, 2.0 grams of sodium acetate, 2.0 grams of ammonium chloride, 2.0 grams of potassium dihydrogen phosphate, 0.2 grams of calcium chloride, 6.0 grams of sodium bicarbonate, 1.0 grams of magnesium chloride, and trace element solution into 2 liters of deionized water 2.0mL, vitamin solution 2.0mL, yeast extract 1.0g, to fully dissolve each component; then adjust the pH value to 6.8-7.2 with 5% sodium hydroxide solution by weight percentage, and then put it into a conical flask to obtain an expanded culture Base; sterilize the expansion medium at a temperature of 121° C. for 30 minutes to obtain a sterilized expansion medium;

[0045] When the sterilized expansion medium is cooled to 30°C-35°C, insert Rhodopseudomonas palustris according to 10% of the volume of the sterilized expansion medium ( Rhodopeudomonas palustris Strain preservation number CGMCC1.2352), fermented for 3 days at a temperature of 27°C and a light intensity of 2200 lux to o...

Embodiment 2

[0051] Add 1.0 g of sodium malate, 1.0 g of sodium acetate, 1.0 g of ammonium chloride, 1.0 g of potassium dihydrogen phosphate, 0.1 g of calcium chloride, 3.0 g of sodium bicarbonate, 0.5 g of magnesium chloride, and trace element solution into 1 liter of deionized water 1.0mL, vitamin solution 1.0mL, yeast extract 0.5g, to fully dissolve each component; then adjust the pH value to 6.8-7.2 with 5% sodium hydroxide solution by weight percentage, and then put it into a conical flask to obtain an expanded culture Base; sterilize the expansion medium at a temperature of 121° C. for 30 minutes to obtain a sterilized expansion medium;

[0052] When the sterilized expansion medium is cooled to 30°C-35°C, insert Rhodopseudomonas palustris according to 11% of the volume of the sterilized expansion medium ( Rhodopeudomonas palustris Strain preservation number CGMCC1.2352), fermented for 3 days at a temperature of 27°C and a light intensity of 2800 lux to obtain a reddish-brown strain ...

Embodiment 3

[0056] Add 1.0 g of sodium malate, 1.0 g of sodium acetate, 1.0 g of ammonium chloride, 1.0 g of potassium dihydrogen phosphate, 0.1 g of calcium chloride, 3.0 g of sodium bicarbonate, 0.5 g of magnesium chloride, and trace element solution into 1 liter of deionized water 1.0mL, vitamin solution 1.0mL, yeast extract 0.5g, to fully dissolve each component; then adjust the pH value to 6.8-7.2 with 5% sodium hydroxide solution by weight percentage, and then put it into a conical flask to obtain an expanded culture Base; sterilize the expansion medium at a temperature of 121° C. for 30 minutes to obtain a sterilized expansion medium;

[0057] When the sterilized expanded medium was cooled to 30°C-35°C, 9% of the volume of the sterilized expanded medium was inserted into Rhodopseudomonas palustris ( Rhodopeudomonas palustris Strain preservation number CGMCC1.2352), fermented for 4 days at a temperature of 27°C and a light intensity of 1500 lux to obtain a reddish-brown strain cult...

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Abstract

The invention discloses a method for producing an aerobic fermentation photosynthetic bacteria livestock culturing feed additive, belonging to the field of microbial fermentation. The technical problem to be solved of the invention is to provide a method for producing an aerobic fermentation photosynthetic bacteria livestock culturing feed additive which has stable quality and low energy consumption. The adopted technical scheme comprises the followings steps of: performing strain amplification culture, inoculating marsh rhodopseudomonas in an amount 8-12 percent based on the volume of an amplification culture medium, and fermenting at the temperature 27 DEG C and the light intensity 1,000-3,000 lux for 3-4 days to obtain a strain culture fluid; performing fermentation production: inoculating bacteria in an amount 10-18 percent based on the volume of the fermentation culture medium at the temperature 29 DEG C, stirring at the speed 100 r / min, keeping the solved oxygen at 1.8 mg / L in a tank with sterile air, and keeping the internal pressure of the tank at 0.02-0.04MPa; and fermenting for 5 days under the dark condition to obtain the photosynthetic bacteria livestock culturing feed additive. Compared with the prior art, the method has the advantage that: the energy consumption of fermentation production is lowered.

Description

technical field [0001] The invention discloses a method for producing aerobic fermentation photosynthetic bacteria feed additive for livestock and poultry breeding, which belongs to the field of microbial fermentation. Background technique [0002] Photosynthetic bacteria are a group of bacteria that can use organic matter as oxygen donor and carbon source under anaerobic light or aerobic dark conditions. They have been widely used in crops, livestock and poultry breeding, aquaculture, environmental protection and other fields. At present, a variety of photosynthetic bacteria or methods for livestock and poultry breeding feed additives containing photosynthetic bacteria have been developed. These methods are mainly developed based on the principle that photosynthetic bacteria can grow under anaerobic light conditions. Chinese Invention Patent No. 200910039525.5 discloses a technology called "A Production Method for Agricultural Photosynthetic Bacteria Preparation". This pho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16C12N1/20C12R1/01A23K10/16
CPCY02A40/70
Inventor 李保珍张肇铭吉海赵利华
Owner SHANXI WEIMINSHENG TECH
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