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High-yield culture medium using dickeyazeae to produce antibiotic zeamine and culture method

A technology of rice basal rot fungus and culture medium, which is applied in the field of biological high, can solve the problems of low zeamine yield, cumbersome medium preparation process, unfavorable antibiotic extraction, etc., and achieves the advantages of large-scale production, easy control of fermentation conditions and low cost. Effect

Active Publication Date: 2014-04-30
SOUTH CHINA AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a high-yield culture medium for rice base rot antibiotic zeamine in order to overcome the defects in the prior art that the yield of zeamine in the rice base rot pathogen culture medium is low, the culture medium preparation process is cumbersome, and it is not conducive to the extraction of antibiotics

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  • High-yield culture medium using dickeyazeae to produce antibiotic zeamine and culture method
  • High-yield culture medium using dickeyazeae to produce antibiotic zeamine and culture method

Examples

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Effect test

Embodiment 1

[0020] Example 1 Exploration of the components of the rice base rot antibiotic zeamine high-yield medium

[0021] 1. Materials and methods

[0022] 1. Strains: The strains of rice base rot pathogens are provided by Guangdong Key Laboratory of Microbial Signaling and Crop Disease Prevention and Control.

[0023] 2. Cultivation method: Shake a 250mL triangular conical flask with a liquid volume of 100mL, and inoculate 100μL of rice base rot fungus with a concentration of OD1.0 Dickeya.zeae Bacteria Seed Liquid. Set up a horizontal constant temperature shaker with a rotation speed of 100 rpm and a temperature of 28-30°C. Each test was repeated three times, and the results were averaged.

[0024] 3. Antibiotic content determination method: After sample preparation and fermentation, take 10 mL of the culture solution, 100 ° C boiling water bath for 10 minutes, 12000 rpm, separate and precipitate for 5 minutes, and take the supernatant for biological determination of antibiotic c...

Embodiment 2

[0049] The rice base rot pathogen strains were provided by Guangdong Key Laboratory of Microbial Signaling and Crop Disease Prevention and Control.

[0050] The content of each component of rice base rot antibiotic zeamine high-yield medium 1L is: 1.4g ammonium nitrate, 12.7g sucrose, 9.25g dipotassium hydrogen phosphate, 3.3g potassium dihydrogen phosphate, 1g potassium chloride, 1g magnesium sulfate, 1g Asparagine.

[0051] Culture medium preparation method: weigh each component according to the above medium formula, add water and stir evenly, adjust the pH to 7, put the medium into a 200mL / 400mL / 1000mL blue bottle, and sterilize at 0.1Mpa for 20 minutes.

[0052] The method for high-yield cultivation of the rice base rot pathogen antibiotic zeamine using the above-mentioned medium: Inoculate the rice base rot pathogen antibiotic zeamine in the medium, control the temperature of the shaker flask at 28°C, the filling volume is 200mL / 500mL, the optimum pH is 7.0, and the rotat...

Embodiment 3

[0055] The content of 1L high-yield medium for rice base rot antibiotic zeamine is: 4.5g ammonium nitrate, 10g sucrose, 5g dipotassium hydrogen phosphate, 2.5g potassium dihydrogen phosphate, 3g potassium chloride, 0.7g magnesium sulfate, 4.5g asparagus amides.

[0056] Culture medium preparation method: with embodiment 2.

[0057] The method for high-yield cultivation of the rice base rot pathogen antibiotic zeamine using the above-mentioned medium: the same as in Example 2.

[0058] The antibiotic yield obtained by using the medium described in Example 3 is 20 times that obtained by using the MM medium.

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Abstract

The invention discloses a high-yield culture medium using dickeyazeae to produce broad-spectrum antibiotic zeamine and a culture method. 1L of the culture medium contains 1-30g of a carbon source, 0.5-5g of a nitrogen source, 0.05-5g of an inorganic salt of and 0.1-5g of an amino acid. The carbon source is one or a plurality of glucose, sucrose, mannitol, glycerin or fructose; the nitrogen source is one kind or a plurality of ammonium sulfate, sodium nitrate, ammonium nitrate or ammonium chloride; the inorganic salt is one or a plurality of ferrous sulfate, calcium chloride, manganese chloride, magnesium sulfate or potassium chloride; the amino acid is one or a plurality of aspartic acid, asparagine or proline. The yield of the antibiotic produced by use of the high-yield culture medium is 20-30 times more than the antibiotic produced by use of a basic culture medium, the high-yield culture medium is simple in components, simple in preparation, low in cost, and easy in purification and separation of the antibiotic, and the culture method has the advantages of simple operation and easy control of culture conditions, and is beneficial for large-scale preparation of the antibiotic.

Description

Technical field [0001] The present invention involves high -tech fields, especially a medium and culture method for bacterial culture culture. It specifically involves a high -yield medium and training method for the rice -based rot fungus antibiotics ZEAMINE. Background technique [0002] Rice -based rot bacteria ( Dickeya. Zeae ) The bacteria are single, short rod -shaped, dull at both ends, and the size is 0.7 μm × 1.4 ~ 1.75 μm.Gram -dyeing reactions are negative and do not form spimes and masks.It turned into a fresh -yellow yellow, a slightly shrinking surface, no gloss (Liu Qiongguang et al., 1997).There is no fluorescence reaction in the Jin's medium, growing qi and growing. The type of disease is soft rot, which can rot potato chips and emit a bad odor (Hong Jianming et al., 1983).Recent research results show that a new type of antibiotic Zeamine produces a new type of antibiotic Zeamine. From the perspective of chemical structure, Zeamine is a polymerizer compound. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12N1/20C12R1/01
Inventor 张炼辉廖立胜周佳暖陈少华
Owner SOUTH CHINA AGRI UNIV
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