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Multi-stage liquid submerged fermentation method of Ganoderma lucidum

A liquid deep fermentation, multi-stage technology, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems affecting the quality stability of Ganoderma lucidum triterpenes, long production cycle of Ganoderma lucidum triterpenes, etc., and achieve the fermentation process. Simple and easy to implement, easy to control fermentation conditions, fast effect

Active Publication Date: 2020-07-10
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obtaining Ganoderma lucidum triterpenes through cultivation has a long production cycle, and is greatly affected by the environment and seasonal climate, which seriously affects the quality stability of the obtained Ganoderma lucidum triterpenes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 3L fermenter multi-stage fermentation production of Ganoderma lucidum:

[0040] The pH value of the fermentation broth was controlled by feeding hydrochloric acid (2moL / L) or sodium hydroxide (2moL / L) during the fermentation process.

[0041] (1) The first stage of fermentation (0-26h), control the fermentation pH to the original pH of the medium (about 4.8-5.0), the temperature is 26°C, and the stirring speed is 80r / min; (2) The second stage of fermentation (26-36h ), the fermentation pH is controlled to be 4.0, the temperature is 26°C, and the stirring speed is 80r / min; (3) the third stage of fermentation (36-81h), the fermentation pH is controlled to be 4.5, the temperature is 26°C, and the stirring speed is 80r / min; ( 4) In the fourth stage of fermentation (81h-168h), the fermentation pH is controlled to be 5.0, the temperature is 26°C, the stirring speed is 80r / min, and the fermentation is completed until 168h.

[0042] Extraction of Ganoderma lucidum triterpenes:...

Embodiment 2

[0053] During the fermentation process, the pH value of the fermentation broth was controlled by feeding hydrochloric acid (2moL / L) or sodium hydroxide (2moL / L).

[0054] 50L fermenter multi-stage fermentation production of Ganoderma lucidum triterpenes:

[0055] (1) The first stage of fermentation (0-26h), control the fermentation pH to be natural, the temperature is 26 ° C, and the stirring speed is 80r / min; (2) The second stage of fermentation (26-36h), control the fermentation pH to 4.0, the temperature (3) The third stage of fermentation (36-81h), control the fermentation pH to 4.5, the temperature is 26°C, and the stirring speed is 80r / min; (4) The fourth stage of fermentation (81h- 168h), the fermentation pH was controlled to be 5.0, the temperature was 26°C, the stirring speed was 80r / min, and the fermentation was completed until 168h.

[0056]The extraction and determination method of Ganoderma lucidum triterpenoids is the same as in Example 1, and the yield of Ganod...

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Abstract

The invention discloses a multi-stage liquid submerged fermentation method of Ganoderma lucidum. The method comprises the following steps: (1) a first fermentation stage (0-26 h), wherein fermentationhas a pH of the original pH of a culture medium, a temperature of 26 DEG C, and a stirring speed of 80 r / min; (2) a second fermentation stage (26-36 h), controlling the pH to 4.0, temperature to 26 DEG C, and stirring speed at 80 r / min for fermentation; (3) a third fermentation stage (36-81 h), controlling the pH to 4.5, temperature to 26 DEG C, and stirring speed at 80 r / min for fermentation; and (4) a fourth fermentation stage (81-168 h), controlling the pH to 5.0, temperature to 26 DEG C, and stirring speed at 80 r / min for fermentation, and ending the fermentation at 168 h. The Ganoderma lucidum mycelium produced by the method has a significantly increased content of ganoderma triterpenes in mycelium.

Description

technical field [0001] The invention relates to the field of edible fungus production, in particular to a multi-stage liquid submerged fermentation method of ganoderma lucidum. Background technique [0002] Ganoderma lucidum triterpenes are important bioactive products in Ganoderma lucidum. For decades, increasing the production of Ganoderma lucidum triterpenes has been the goal of researchers due to the huge market demand. At present, there are two ways to obtain Ganoderma lucidum triterpenoids, one is to obtain from fruiting body and spore powder, and the other is to obtain from Ganoderma lucidum fermentation broth or mycelia. Obtaining ganoderma lucidum triterpenes through cultivation has a long production cycle and is greatly affected by the environment and seasonal climate, which seriously affects the quality stability of the obtained ganoderma lucidum triterpenes. [0003] Therefore, it is necessary to develop a new cultivation method of Ganoderma lucidum, thereby in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12N1/14C12R1/645
CPCC12P33/00C12N1/14
Inventor 冯杰唐庆九潘江安张劲松冯娜周帅唐传红谭贻刘艳芳王金艳刘方
Owner SHANGHAI ACAD OF AGRI SCI
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