Biocontrol microorganism capable of generating plant pathogenic fungi-resistant lipopeptide, and applications of pesticide preparation of biocontrol microorganism

Abstract

The invention relates to a biocontrol microorganism capable of separating from sea mud products and generating plant pathogenic fungi-resistant lipopeptide, and applications of the biocontrol microorganism. The microorganism is a Bacillus licheniformis 9912 strain (in China Center For Type Culture Collection, with collection number of CCTCC No. M2010343). The microorganism is fermented to generate cyclic lipopeptide compounds 6-Abu C16fengycin, anteiso-C15surfactin and anteiso-C15surfactin methyl ester which have wide-spectrum plant pathogenic fungi prevention bioactivity as main metabolite; the microorganism and the fermented metabolite can conventionally adsorb a carrier and auxiliaries to prepare a microbial preparation and a biopesticide preparation capable of effectively preventing and treating cucumber and botrytis cinerea, blight, tomato stem rot, apple tree canker, valsa mali miyabe et yamada, pear tree blight and the like, and have the characteristics of being low in cost, efficient in biocontrol, non-residual, and safe to human and livestock.

Description

technical field [0001] The invention relates to a biocontrol microorganism which is isolated from a sea mud sample and produces lipopeptides against plant pathogenic fungi and its application. The microorganism of the present invention is Bacillus licheniformis 9912 strain (China Center for Type Culture Collection, preservation number CCTCC No. M2010343). The fermentation of the bacterium mainly produces cyclolipopeptide compounds 6-Abu C16fengycin, anteiso-C15surfactin, anteiso-C15surfactin methyl ester, which have broad-spectrum antifungal biological activity. The bacterium and the above-mentioned fermentation metabolites can be made to effectively control cucumber ash Mildew, fusarium wilt and other plant pathogenic fungal diseases are used as biological pesticides, microbial preparations and plant rhizosphere growth-promoting bacteria agents. Background technique [0002] In recent years, the research on lipopeptides derived from microorganisms has made great progress i...

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Problems solved by technology

The recent identification found that it is consistent with the characteristics of Bacilus methylotrophicus, which is Munusamy Madhaiyan et al. in 2010 (Madhaiyan M., Poonguzhali S., Kwon S.W., et al.Bacilus lincheniformis sp.nov., a methanol-utilizing, plant-growth -promoting bacterium isolated from rice rhizosphere soil, International Journal of Systematic and Evolutionary Microbiology, 2010.60(10):p.2490-5) was isolated from rice root soil and was identified as a new species for the first time, but as a plant rhizosphere growth-promoting Bacteria, no possible antibacterial active substances were reported; Mo Minghe et al. (Mo Minghe, Lin Bilian, Huang Ying, etc. A broad-spectrum antagonizing plant pathogenic fungus biocontrol microorganism and its application, Chinese invention patent. CN102120969A.2011.) Applying for an invention patent applied to the control of fungal diseases of crops has not clarified the antibacterial substances and mechanism of action

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