The invention discloses a cosmetic preparation rich in cell growth factors. The cosmetic preparation is prepared from the following components: 0.01 to 0.2 percent of STP, 0.01 to 0.1 percent of cellgrowth factors, 1 to 10 percent of fish oil, 1 to 10 percent of surfactant, 1 to 15percent of humectant, 0.1 to 1 percent of antioxidant, 0.1 to 3 percent of pH modifier, 3 to 5 percent of emulsifier,0.1 to 5 percent of natural plant extract and deionized water. The invention provides the cosmetic preparation rich in the cell growth factors; the cosmetic preparation has the advantages that aged cells can be constantly replaced with new cells, so that the effects of preventing and removing wrinkles are generated through skin rejuvenation; skin wrinkles, dryness, sagging, aging and light damagecan be prevented, or the texture and smoothness of the skin are improved; the emulsification effect of cosmetics can be enhanced and the stability of cosmetics is improved. The invention also provides the preparation method of the cosmetic preparation rich in the cell growth factors; the preparation method has the advantages of simple operation, simplicity and easiness in obtaining materials, lowcost and worthiness of being popularized.