A hydrotalcite composite luminescent material with rhodamine intercalation, which pertains to abio-organic composite luminescent material. The process is preparing solution A with mole ratio of bivalence and tervalence cation M2+ / M3+=2.0-4.0; adding laser dye sulphonated rhodamine and sodium salt of dodecylbenzene sulfonic acid into mixed solution of ethanol and water and fully dissolving to obtain solution of sulphonated rhodamine and dodecyl benzene sulfone acid group containing anion with negative charge B; mixing solution A, B into solution C, preparing NaOH solution; communicating solution C and NaOH solution into full-back-liquid mixing membrane reactor to react, mixing to obtain red slurry, introducing the red slurry into hydrothermal kettle, reacting, absterging by CO2, de-ionized water and absolute ethyl alcohol and centrifugally separating, vacuum drying. Then hydrotalcite system with co-intercalation of suphonated rhodamine and dodecyl sulfone acid group is obtained. Advantages of the invention are that dye molecular is immobilized, and fluorescence quenching caused by dye aggregation is reduced.